Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii
Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance,...
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doaj-44f72f1389224142ab95a83e5aa920192020-11-24T22:52:38ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-09-01810.3389/fpls.2017.01642283733Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersoniiFederico Boschi0Claudia Schvartzman1Sara Murchio2Virginia Ferreira3Maria I. Siri4Guillermo A. Galván5Matthew Smoker6Lena Stransfeld7Cyril Zipfel8Francisco L. Vilaró9Marco Dalla-Rizza10Instituto Nacional de SemillasCanelones, UruguayUnidad de Biotecnología, Instituto Nacional de Investigación AgropecuariaCanelones, UruguayUnidad de Biotecnología, Instituto Nacional de Investigación AgropecuariaCanelones, UruguayDepartamento de Biociencias, Facultad de Química, Universidad de la RepúblicaMontevideo, UruguayDepartamento de Biociencias, Facultad de Química, Universidad de la RepúblicaMontevideo, UruguayDepartamento de Producción Vegetal, Centro Regional Sur, Facultad de Agronomía, Universidad de la RepúblicaCanelones, UruguayThe Sainsbury Laboratory, Norwich Research ParkNorwich, United KingdomThe Sainsbury Laboratory, Norwich Research ParkNorwich, United KingdomThe Sainsbury Laboratory, Norwich Research ParkNorwich, United KingdomPrograma de Producción Hortícola, Instituto Nacional de Investigación AgropecuariaCanelones, UruguayUnidad de Biotecnología, Instituto Nacional de Investigación AgropecuariaCanelones, UruguayBacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of AtEFR with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato.http://journal.frontiersin.org/article/10.3389/fpls.2017.01642/fullRalstonia solanacearumbacterial wiltSolanum tuberosumSolanum commersoniipattern recognition receptorEFR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Federico Boschi Claudia Schvartzman Sara Murchio Virginia Ferreira Maria I. Siri Guillermo A. Galván Matthew Smoker Lena Stransfeld Cyril Zipfel Francisco L. Vilaró Marco Dalla-Rizza |
spellingShingle |
Federico Boschi Claudia Schvartzman Sara Murchio Virginia Ferreira Maria I. Siri Guillermo A. Galván Matthew Smoker Lena Stransfeld Cyril Zipfel Francisco L. Vilaró Marco Dalla-Rizza Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii Frontiers in Plant Science Ralstonia solanacearum bacterial wilt Solanum tuberosum Solanum commersonii pattern recognition receptor EFR |
author_facet |
Federico Boschi Claudia Schvartzman Sara Murchio Virginia Ferreira Maria I. Siri Guillermo A. Galván Matthew Smoker Lena Stransfeld Cyril Zipfel Francisco L. Vilaró Marco Dalla-Rizza |
author_sort |
Federico Boschi |
title |
Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii |
title_short |
Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii |
title_full |
Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii |
title_fullStr |
Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii |
title_full_unstemmed |
Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii |
title_sort |
enhanced bacterial wilt resistance in potato through expression of arabidopsis efr and introgression of quantitative resistance from solanum commersonii |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2017-09-01 |
description |
Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of AtEFR with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato. |
topic |
Ralstonia solanacearum bacterial wilt Solanum tuberosum Solanum commersonii pattern recognition receptor EFR |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2017.01642/full |
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