High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA1

High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA1

We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP‐PCR) as opposed to the use of unamplified DNA. Five DNA samples from B‐cell leukemias with small 11q deletions we...

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Main Authors: Jacob Larsen, Anne Marie Ottesen, Maria Kirchhoff, Claes Lundsteen, Jørgen K. Larsen
Format: Article
Language:English
Published: Hindawi Limited 2001-01-01
Series:Analytical Cellular Pathology
Online Access:http://dx.doi.org/10.1155/2001/301570
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spelling doaj-450c3c3a71144e698cc26dbfe643559f2020-11-24T22:08:35ZengHindawi LimitedAnalytical Cellular Pathology0921-89121878-36512001-01-01232616410.1155/2001/301570High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA1Jacob Larsen0Anne Marie Ottesen1Maria Kirchhoff2Claes Lundsteen3Jørgen K. Larsen4Finsen Laboratory, Finsen Center, Rigshospitalet, Strandboulevarden 49, DK‐2100, Copenhagen, DenmarkDepartment of Growth and Reproduction, Juliane Marie Center, Rigshospitalet, Blegdamsvej 9, DK‐2100, Copenhagen, DenmarkDepartment of Clinical Genetics, Juliane Marie Center, Rigshospitalet, Blegdamsvej 9, DK‐2100, Copenhagen, DenmarkDepartment of Clinical Genetics, Juliane Marie Center, Rigshospitalet, Blegdamsvej 9, DK‐2100, Copenhagen, DenmarkFinsen Laboratory, Finsen Center, Rigshospitalet, Strandboulevarden 49, DK‐2100, Copenhagen, DenmarkWe investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP‐PCR) as opposed to the use of unamplified DNA. Five DNA samples from B‐cell leukemias with small 11q deletions were amplified by DOP‐PCR and analysed by means of high resolution comparative genomic hybridization (HR‐CGH) for the evaluation of aberration size detection limit. By means of HR‐CGH, we found the detection limit of DOP‐PCR CGH for deletions to be between 3 Mbp and 7–8 Mbp.http://dx.doi.org/10.1155/2001/301570
collection DOAJ
language English
format Article
sources DOAJ
author Jacob Larsen
Anne Marie Ottesen
Maria Kirchhoff
Claes Lundsteen
Jørgen K. Larsen
spellingShingle Jacob Larsen
Anne Marie Ottesen
Maria Kirchhoff
Claes Lundsteen
Jørgen K. Larsen
High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA1
Analytical Cellular Pathology
author_facet Jacob Larsen
Anne Marie Ottesen
Maria Kirchhoff
Claes Lundsteen
Jørgen K. Larsen
author_sort Jacob Larsen
title High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA1
title_short High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA1
title_full High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA1
title_fullStr High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA1
title_full_unstemmed High Resolution Comparative Genomic Hybridization Detects 7–8 Megabasepair Deletion in PCR Amplified DNA1
title_sort high resolution comparative genomic hybridization detects 7–8 megabasepair deletion in pcr amplified dna1
publisher Hindawi Limited
series Analytical Cellular Pathology
issn 0921-8912
1878-3651
publishDate 2001-01-01
description We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP‐PCR) as opposed to the use of unamplified DNA. Five DNA samples from B‐cell leukemias with small 11q deletions were amplified by DOP‐PCR and analysed by means of high resolution comparative genomic hybridization (HR‐CGH) for the evaluation of aberration size detection limit. By means of HR‐CGH, we found the detection limit of DOP‐PCR CGH for deletions to be between 3 Mbp and 7–8 Mbp.
url http://dx.doi.org/10.1155/2001/301570
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