N-acetyl-cysteine inhibits liver oxidative stress markers in BALB/c mice infected with Leishmania amazonensis
BACKGROUND Leishmaniasis is a parasitosis caused by several species of the genus Leishmania. These parasites present high resistance against oxidative stress generated by inflammatory cells. OBJECTIVES To investigate oxidative stress and molecular inflammatory markers in BALB/c mice infected with...
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Instituto Oswaldo Cruz, Ministério da Saúde
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doaj-453f2fb9b84e4675b832bb713927d0ce2020-11-25T01:02:48ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.1678-8060112214615410.1590/0074-02760160403S0074-02762017000200146N-acetyl-cysteine inhibits liver oxidative stress markers in BALB/c mice infected with Leishmania amazonensisJuciano GasparottoAlice KunzlerMario Roberto SengerCeleste da Silva Freitas de SouzaSalvatore Giovanni de SimoneRafael Calixto BortolinNauana SomensiFelipe Dal-PizzolJosé Claudio Fonseca MoreiraAna Lúcia Abreu-SilvaKátia da Silva CalabreseFloriano Paes Silva JrDaniel Pens GelainBACKGROUND Leishmaniasis is a parasitosis caused by several species of the genus Leishmania. These parasites present high resistance against oxidative stress generated by inflammatory cells. OBJECTIVES To investigate oxidative stress and molecular inflammatory markers in BALB/c mice infected with L. amazonensis and the effect of antioxidant treatment on these parameters. METHODS Four months after infection, oxidative and inflammatory parameters of liver, kidneys, spleen, heart and lungs from BALB/c mice were assessed. FINDINGS In liver, L. amazonensis caused thiol oxidation and nitrotyrosine formation; SOD activity and SOD2 protein content were increased while SOD1 protein content decreased. The content of the cytokines IL-1β, IL-6, TNF-α, and the receptor of advanced glycation endproducts (RAGE) increased in liver. Treatment with the antioxidant N-acetyl-cysteine (20 mg/kg b.w) for five days inhibited oxidative stress parameters. MAIN CONCLUSIONS L. amazonensis induces significant alterations in the redox status of liver but not in other organs. Acute antioxidant treatment alleviates oxidative stress in liver, but it had no effect on pro-inflammatory markers. These results indicate that the pathobiology of leishmaniasis is not restricted to the cutaneous manifestations and open perspectives for the development of new therapeutic approaches to the disease, especially for liver function.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000200146&lng=en&tlng=enLeishmania amazonensisantioxidant treatmentliver damage |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juciano Gasparotto Alice Kunzler Mario Roberto Senger Celeste da Silva Freitas de Souza Salvatore Giovanni de Simone Rafael Calixto Bortolin Nauana Somensi Felipe Dal-Pizzol José Claudio Fonseca Moreira Ana Lúcia Abreu-Silva Kátia da Silva Calabrese Floriano Paes Silva Jr Daniel Pens Gelain |
spellingShingle |
Juciano Gasparotto Alice Kunzler Mario Roberto Senger Celeste da Silva Freitas de Souza Salvatore Giovanni de Simone Rafael Calixto Bortolin Nauana Somensi Felipe Dal-Pizzol José Claudio Fonseca Moreira Ana Lúcia Abreu-Silva Kátia da Silva Calabrese Floriano Paes Silva Jr Daniel Pens Gelain N-acetyl-cysteine inhibits liver oxidative stress markers in BALB/c mice infected with Leishmania amazonensis Memórias do Instituto Oswaldo Cruz. Leishmania amazonensis antioxidant treatment liver damage |
author_facet |
Juciano Gasparotto Alice Kunzler Mario Roberto Senger Celeste da Silva Freitas de Souza Salvatore Giovanni de Simone Rafael Calixto Bortolin Nauana Somensi Felipe Dal-Pizzol José Claudio Fonseca Moreira Ana Lúcia Abreu-Silva Kátia da Silva Calabrese Floriano Paes Silva Jr Daniel Pens Gelain |
author_sort |
Juciano Gasparotto |
title |
N-acetyl-cysteine inhibits liver oxidative stress markers in BALB/c mice infected with Leishmania amazonensis |
title_short |
N-acetyl-cysteine inhibits liver oxidative stress markers in BALB/c mice infected with Leishmania amazonensis |
title_full |
N-acetyl-cysteine inhibits liver oxidative stress markers in BALB/c mice infected with Leishmania amazonensis |
title_fullStr |
N-acetyl-cysteine inhibits liver oxidative stress markers in BALB/c mice infected with Leishmania amazonensis |
title_full_unstemmed |
N-acetyl-cysteine inhibits liver oxidative stress markers in BALB/c mice infected with Leishmania amazonensis |
title_sort |
n-acetyl-cysteine inhibits liver oxidative stress markers in balb/c mice infected with leishmania amazonensis |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
1678-8060 |
description |
BACKGROUND Leishmaniasis is a parasitosis caused by several species of the genus Leishmania. These parasites present high resistance against oxidative stress generated by inflammatory cells. OBJECTIVES To investigate oxidative stress and molecular inflammatory markers in BALB/c mice infected with L. amazonensis and the effect of antioxidant treatment on these parameters. METHODS Four months after infection, oxidative and inflammatory parameters of liver, kidneys, spleen, heart and lungs from BALB/c mice were assessed. FINDINGS In liver, L. amazonensis caused thiol oxidation and nitrotyrosine formation; SOD activity and SOD2 protein content were increased while SOD1 protein content decreased. The content of the cytokines IL-1β, IL-6, TNF-α, and the receptor of advanced glycation endproducts (RAGE) increased in liver. Treatment with the antioxidant N-acetyl-cysteine (20 mg/kg b.w) for five days inhibited oxidative stress parameters. MAIN CONCLUSIONS L. amazonensis induces significant alterations in the redox status of liver but not in other organs. Acute antioxidant treatment alleviates oxidative stress in liver, but it had no effect on pro-inflammatory markers. These results indicate that the pathobiology of leishmaniasis is not restricted to the cutaneous manifestations and open perspectives for the development of new therapeutic approaches to the disease, especially for liver function. |
topic |
Leishmania amazonensis antioxidant treatment liver damage |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000200146&lng=en&tlng=en |
work_keys_str_mv |
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