MtMTP2-Facilitated Zinc Transport Into Intracellular Compartments Is Essential for Nodule Development in Medicago truncatula
Zinc (Zn) is an essential nutrient for plants that is involved in almost every biological process. This includes symbiotic nitrogen fixation, a process carried out by endosymbiotic bacteria (rhizobia) living within differentiated plant cells of legume root nodules. Zn transport in nodules involves d...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2018-07-01
|
Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.00990/full |
id |
doaj-4543ea695aa74b38a6582f228fd778bf |
---|---|
record_format |
Article |
spelling |
doaj-4543ea695aa74b38a6582f228fd778bf2020-11-24T21:16:22ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-07-01910.3389/fpls.2018.00990371050MtMTP2-Facilitated Zinc Transport Into Intracellular Compartments Is Essential for Nodule Development in Medicago truncatulaJavier León-Mediavilla0Marta Senovilla1Jesús Montiel2Patricia Gil-Díez3Ángela Saez4Igor S. Kryvoruchko5María Reguera6Michael K. Udvardi7Juan Imperial8Juan Imperial9Manuel González-Guerrero10Manuel González-Guerrero11Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, Madrid, SpainCentro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, Madrid, SpainCentro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, Madrid, SpainCentro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, Madrid, SpainCentro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, Madrid, SpainNoble Research Institute, Ardmore, OK, United StatesCentro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, Madrid, SpainNoble Research Institute, Ardmore, OK, United StatesCentro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, Madrid, SpainInstituto de Ciencias Ambientales, Consejo Superior de Investigaciones Científicas, Madrid, SpainCentro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, Madrid, SpainEscuela Técnica Superior de Ingeniería Agronómica, Alimentaría y de Biosistemas, Universidad Politécnica de Madrid (UPM), Madrid, SpainZinc (Zn) is an essential nutrient for plants that is involved in almost every biological process. This includes symbiotic nitrogen fixation, a process carried out by endosymbiotic bacteria (rhizobia) living within differentiated plant cells of legume root nodules. Zn transport in nodules involves delivery from the root, via the vasculature, release into the apoplast and uptake into nodule cells. Once in the cytosol, Zn can be used directly by cytosolic proteins or delivered into organelles, including symbiosomes of infected cells, by Zn efflux transporters. Medicago truncatula MtMTP2 (Medtr4g064893) is a nodule-induced Zn-efflux protein that was localized to an intracellular compartment in root epidermal and endodermal cells, as well as in nodule cells. Although the MtMTP2 gene is expressed in roots, shoots, and nodules, mtp2 mutants exhibited growth defects only under symbiotic, nitrogen-fixing conditions. Loss of MtMTP2 function resulted in altered nodule development, defects in bacteroid differentiation, and severe reduction of nitrogenase activity. The results presented here support a role of MtMTP2 in intracellular compartmentation of Zn, which is required for effective symbiotic nitrogen fixation in M. truncatula.https://www.frontiersin.org/article/10.3389/fpls.2018.00990/fullzinccation diffusion facilitatormetal transport proteinsymbiotic nitrogen fixationmetal nutritionnodulation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Javier León-Mediavilla Marta Senovilla Jesús Montiel Patricia Gil-Díez Ángela Saez Igor S. Kryvoruchko María Reguera Michael K. Udvardi Juan Imperial Juan Imperial Manuel González-Guerrero Manuel González-Guerrero |
spellingShingle |
Javier León-Mediavilla Marta Senovilla Jesús Montiel Patricia Gil-Díez Ángela Saez Igor S. Kryvoruchko María Reguera Michael K. Udvardi Juan Imperial Juan Imperial Manuel González-Guerrero Manuel González-Guerrero MtMTP2-Facilitated Zinc Transport Into Intracellular Compartments Is Essential for Nodule Development in Medicago truncatula Frontiers in Plant Science zinc cation diffusion facilitator metal transport protein symbiotic nitrogen fixation metal nutrition nodulation |
author_facet |
Javier León-Mediavilla Marta Senovilla Jesús Montiel Patricia Gil-Díez Ángela Saez Igor S. Kryvoruchko María Reguera Michael K. Udvardi Juan Imperial Juan Imperial Manuel González-Guerrero Manuel González-Guerrero |
author_sort |
Javier León-Mediavilla |
title |
MtMTP2-Facilitated Zinc Transport Into Intracellular Compartments Is Essential for Nodule Development in Medicago truncatula |
title_short |
MtMTP2-Facilitated Zinc Transport Into Intracellular Compartments Is Essential for Nodule Development in Medicago truncatula |
title_full |
MtMTP2-Facilitated Zinc Transport Into Intracellular Compartments Is Essential for Nodule Development in Medicago truncatula |
title_fullStr |
MtMTP2-Facilitated Zinc Transport Into Intracellular Compartments Is Essential for Nodule Development in Medicago truncatula |
title_full_unstemmed |
MtMTP2-Facilitated Zinc Transport Into Intracellular Compartments Is Essential for Nodule Development in Medicago truncatula |
title_sort |
mtmtp2-facilitated zinc transport into intracellular compartments is essential for nodule development in medicago truncatula |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2018-07-01 |
description |
Zinc (Zn) is an essential nutrient for plants that is involved in almost every biological process. This includes symbiotic nitrogen fixation, a process carried out by endosymbiotic bacteria (rhizobia) living within differentiated plant cells of legume root nodules. Zn transport in nodules involves delivery from the root, via the vasculature, release into the apoplast and uptake into nodule cells. Once in the cytosol, Zn can be used directly by cytosolic proteins or delivered into organelles, including symbiosomes of infected cells, by Zn efflux transporters. Medicago truncatula MtMTP2 (Medtr4g064893) is a nodule-induced Zn-efflux protein that was localized to an intracellular compartment in root epidermal and endodermal cells, as well as in nodule cells. Although the MtMTP2 gene is expressed in roots, shoots, and nodules, mtp2 mutants exhibited growth defects only under symbiotic, nitrogen-fixing conditions. Loss of MtMTP2 function resulted in altered nodule development, defects in bacteroid differentiation, and severe reduction of nitrogenase activity. The results presented here support a role of MtMTP2 in intracellular compartmentation of Zn, which is required for effective symbiotic nitrogen fixation in M. truncatula. |
topic |
zinc cation diffusion facilitator metal transport protein symbiotic nitrogen fixation metal nutrition nodulation |
url |
https://www.frontiersin.org/article/10.3389/fpls.2018.00990/full |
work_keys_str_mv |
AT javierleonmediavilla mtmtp2facilitatedzinctransportintointracellularcompartmentsisessentialfornoduledevelopmentinmedicagotruncatula AT martasenovilla mtmtp2facilitatedzinctransportintointracellularcompartmentsisessentialfornoduledevelopmentinmedicagotruncatula AT jesusmontiel mtmtp2facilitatedzinctransportintointracellularcompartmentsisessentialfornoduledevelopmentinmedicagotruncatula AT patriciagildiez mtmtp2facilitatedzinctransportintointracellularcompartmentsisessentialfornoduledevelopmentinmedicagotruncatula AT angelasaez mtmtp2facilitatedzinctransportintointracellularcompartmentsisessentialfornoduledevelopmentinmedicagotruncatula AT igorskryvoruchko mtmtp2facilitatedzinctransportintointracellularcompartmentsisessentialfornoduledevelopmentinmedicagotruncatula AT mariareguera mtmtp2facilitatedzinctransportintointracellularcompartmentsisessentialfornoduledevelopmentinmedicagotruncatula AT michaelkudvardi mtmtp2facilitatedzinctransportintointracellularcompartmentsisessentialfornoduledevelopmentinmedicagotruncatula AT juanimperial mtmtp2facilitatedzinctransportintointracellularcompartmentsisessentialfornoduledevelopmentinmedicagotruncatula AT juanimperial mtmtp2facilitatedzinctransportintointracellularcompartmentsisessentialfornoduledevelopmentinmedicagotruncatula AT manuelgonzalezguerrero mtmtp2facilitatedzinctransportintointracellularcompartmentsisessentialfornoduledevelopmentinmedicagotruncatula AT manuelgonzalezguerrero mtmtp2facilitatedzinctransportintointracellularcompartmentsisessentialfornoduledevelopmentinmedicagotruncatula |
_version_ |
1726015753952428032 |