Bioaugmentation of Moving Bed Biofilm Reactor (MBBR) with Achromobacter JL9 for enhanced sulfamethoxazole (SMX) degradation in aquaculture wastewater

• Main Authors: Dong hui Liang, Yongyou Hu, Dongmin Liang, Jianhua Chenga, Yuancai Chena
• Format: Article
• Language: English
• Published: Elsevier 2021-01-01
• Series: Ecotoxicology and Environmental Safety
• Subjects: Sulfamethoxazole (SMX); Nitrogen removal; Antibiotics resistance genes (ARGs); Bioaugmentation; Toxicity
• Online Access: http://www.sciencedirect.com/science/article/pii/S0147651320310964

This study investigated whether bioaugmentation improves sulfamethoxazole (SMX) degradation and nitrogen removal in the Moving Bed Biofilm Reactor (MBBR) system. The effects of the C/N ratio on SMX degradation and nitrogen removal were also evaluated. Using MBBR system operation experiments, the bioaugmented reactor was found to perform more effectively than the non-bioaugmentation reactor, with the highest SMX, nitrate-N, and ammonia-N removal efficiencies of 80.49, 94.70, and 96.09%, respectively. The changes in the sulfonamide resistance genes and bacterial communities were detected at various operating conditions. The results indicate that the diversity of the bacterial communities and the abundance of resistance genes were markedly influenced by bioaugmentation and the C/N ratio, with Achromobacter among the dominant genera in the MBBR system. The bio-toxicity of samples, calculated as the inhibition percentage (IP) toward Escherichia coli, was found to decrease to non-toxic ranges after treatment.