Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus
The influence of glycerol and ornithine feeding on clavulanic acid (CA) production by Streptomyces clavuligerus was investigated. In batch experiments, CA maximum concentration (Cp max) ranged randomly from 430 to 560 mg.L-1, with a maximum increase of 10% in relation to the control run, without orn...
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Brazilian Society of Chemical Engineering
2010-12-01
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doaj-457efece905542b698b5d009d5fb0c882020-11-24T22:49:10ZengBrazilian Society of Chemical EngineeringBrazilian Journal of Chemical Engineering0104-66321678-43832010-12-0127449950610.1590/S0104-66322010000400001Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerusJ. C. TeodoroA. Baptista-NetoM. L. G. C. AraujoC. O. HokkaA. C. BadinoThe influence of glycerol and ornithine feeding on clavulanic acid (CA) production by Streptomyces clavuligerus was investigated. In batch experiments, CA maximum concentration (Cp max) ranged randomly from 430 to 560 mg.L-1, with a maximum increase of 10% in relation to the control run, without ornithine. However, the maximum volumetric productivity of CA (Pp max) of 13.7 mg.L-1.h-1 was obtained with 0.66 g.L-1 of ornithine, 44.2% higher than the Pp max in the control run. In fed-batch experiments, Cp max varied within the narrow range from 1.254 to 1.405 g.L-1, 2.5 times higher than that obtained in the control run. The presence of ornithine increased the Pp max, although it influenced only slightly the Cp max. Concerning glycerol, the highest CA production of 1.6 g.L-1 was obtained in the fed-batch with glycerol and ornithine (180 and 3.7 g.L−1) in a 10-L bioreactor, showing a positive effect of ornithine and glycerol, in the proper proportion (48.6:1), on CA biosynthesis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000400001Clavulanic acidStreptomyces clavuligerusGlycerolOrnithineFed-batch |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J. C. Teodoro A. Baptista-Neto M. L. G. C. Araujo C. O. Hokka A. C. Badino |
spellingShingle |
J. C. Teodoro A. Baptista-Neto M. L. G. C. Araujo C. O. Hokka A. C. Badino Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus Brazilian Journal of Chemical Engineering Clavulanic acid Streptomyces clavuligerus Glycerol Ornithine Fed-batch |
author_facet |
J. C. Teodoro A. Baptista-Neto M. L. G. C. Araujo C. O. Hokka A. C. Badino |
author_sort |
J. C. Teodoro |
title |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus |
title_short |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus |
title_full |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus |
title_fullStr |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus |
title_full_unstemmed |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus |
title_sort |
influence of glycerol and ornithine feeding on clavulanic acid production by streptomyces clavuligerus |
publisher |
Brazilian Society of Chemical Engineering |
series |
Brazilian Journal of Chemical Engineering |
issn |
0104-6632 1678-4383 |
publishDate |
2010-12-01 |
description |
The influence of glycerol and ornithine feeding on clavulanic acid (CA) production by Streptomyces clavuligerus was investigated. In batch experiments, CA maximum concentration (Cp max) ranged randomly from 430 to 560 mg.L-1, with a maximum increase of 10% in relation to the control run, without ornithine. However, the maximum volumetric productivity of CA (Pp max) of 13.7 mg.L-1.h-1 was obtained with 0.66 g.L-1 of ornithine, 44.2% higher than the Pp max in the control run. In fed-batch experiments, Cp max varied within the narrow range from 1.254 to 1.405 g.L-1, 2.5 times higher than that obtained in the control run. The presence of ornithine increased the Pp max, although it influenced only slightly the Cp max. Concerning glycerol, the highest CA production of 1.6 g.L-1 was obtained in the fed-batch with glycerol and ornithine (180 and 3.7 g.L−1) in a 10-L bioreactor, showing a positive effect of ornithine and glycerol, in the proper proportion (48.6:1), on CA biosynthesis. |
topic |
Clavulanic acid Streptomyces clavuligerus Glycerol Ornithine Fed-batch |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000400001 |
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