Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton’s jelly
Introduction. Mesenchymal stem cells (MSCs) are a promising tool for regenerative medicine, but due to the heterogeneity of their populations, different sources and isolation techniques, the characteristics defining MSCs are inconsistent. Objective. The aim of this study was to compare the chara...
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Serbian Medical Society
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doaj-457f1e8c1eee422c980f1b79deabb24d2021-01-02T03:03:44ZengSerbian Medical SocietySrpski Arhiv za Celokupno Lekarstvo0370-81792013-01-011413-417818610.2298/SARH1304178TMesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton’s jellyTrivanović DrenkaKocić JelenaMojsilović SlavkoKrstić AleksandraIlić VesnaOkić-Đorđević IvanaSantibanez Juan FranciscoJovčić GordanaTerzić MilanBugarski DianaIntroduction. Mesenchymal stem cells (MSCs) are a promising tool for regenerative medicine, but due to the heterogeneity of their populations, different sources and isolation techniques, the characteristics defining MSCs are inconsistent. Objective. The aim of this study was to compare the characteristics of MSCs derived from two different human tissues: peripheral blood (PB-MSCs) and umbilical cord Wharton’s Jelly (UC-MSCs). Methods. The PB-MSC and UC-MSC were isolated by adherence to plastic after gradient-density separation or an explant culture method, respectively, and compared regarding their morphology, clonogenic efficiency, proliferating rates, immunophenotype and differentiation potential. Results. MSCs derived from both sources exhibit similar morphology, proliferation capacity and multilineage (osteogenic, chondrogenic, adipogenic and myogenic) differentiation potential. Differences were observed in the clonogenic capacity and the immunophenotype, since UC-MSCs showed higher CFU-F (colony-forming units-fibroblastic) cloning efficiency, as well as higher embryonic markers (Nanog, Sox2, SSEA4) expression. When additional surface antigens were analyzed by flow cytometry (CD44, CD90, CD105, CD33, CD34, CD45, CD11b, CD235a) or immunofluorescent labeling (vimentin, STRO-1 and α-smooth muscle actin), most appeared to have similar epitope profiles irrespective of MSC source. Conclusion. The results obtained demonstrated that both MSCs represent good alternative sources of adult MSCs that could be used in cell therapy applications. [Projekat Ministarstva nauke Republike Srbije, br. 175062]http://www.doiserbia.nb.rs/img/doi/0370-8179/2013/0370-81791304178T.pdfmesenchymal stem cellsperipheral bloodumbilical cordcharacterization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Trivanović Drenka Kocić Jelena Mojsilović Slavko Krstić Aleksandra Ilić Vesna Okić-Đorđević Ivana Santibanez Juan Francisco Jovčić Gordana Terzić Milan Bugarski Diana |
spellingShingle |
Trivanović Drenka Kocić Jelena Mojsilović Slavko Krstić Aleksandra Ilić Vesna Okić-Đorđević Ivana Santibanez Juan Francisco Jovčić Gordana Terzić Milan Bugarski Diana Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton’s jelly Srpski Arhiv za Celokupno Lekarstvo mesenchymal stem cells peripheral blood umbilical cord characterization |
author_facet |
Trivanović Drenka Kocić Jelena Mojsilović Slavko Krstić Aleksandra Ilić Vesna Okić-Đorđević Ivana Santibanez Juan Francisco Jovčić Gordana Terzić Milan Bugarski Diana |
author_sort |
Trivanović Drenka |
title |
Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton’s jelly |
title_short |
Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton’s jelly |
title_full |
Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton’s jelly |
title_fullStr |
Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton’s jelly |
title_full_unstemmed |
Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton’s jelly |
title_sort |
mesenchymal stem cells isolated from peripheral blood and umbilical cord wharton’s jelly |
publisher |
Serbian Medical Society |
series |
Srpski Arhiv za Celokupno Lekarstvo |
issn |
0370-8179 |
publishDate |
2013-01-01 |
description |
Introduction. Mesenchymal stem cells (MSCs) are a promising tool for regenerative medicine, but due to the heterogeneity of their populations, different sources and isolation techniques, the characteristics defining MSCs are inconsistent. Objective. The aim of this study was to compare the characteristics of MSCs derived from two different human tissues: peripheral blood (PB-MSCs) and umbilical cord Wharton’s Jelly (UC-MSCs). Methods. The PB-MSC and UC-MSC were isolated by adherence to plastic after gradient-density separation or an explant culture method, respectively, and compared regarding their morphology, clonogenic efficiency, proliferating rates, immunophenotype and differentiation potential. Results. MSCs derived from both sources exhibit similar morphology, proliferation capacity and multilineage (osteogenic, chondrogenic, adipogenic and myogenic) differentiation potential. Differences were observed in the clonogenic capacity and the immunophenotype, since UC-MSCs showed higher CFU-F (colony-forming units-fibroblastic) cloning efficiency, as well as higher embryonic markers (Nanog, Sox2, SSEA4) expression. When additional surface antigens were analyzed by flow cytometry (CD44, CD90, CD105, CD33, CD34, CD45, CD11b, CD235a) or immunofluorescent labeling (vimentin, STRO-1 and α-smooth muscle actin), most appeared to have similar epitope profiles irrespective of MSC source. Conclusion. The results obtained demonstrated that both MSCs represent good alternative sources of adult MSCs that could be used in cell therapy applications. [Projekat Ministarstva nauke Republike Srbije, br. 175062] |
topic |
mesenchymal stem cells peripheral blood umbilical cord characterization |
url |
http://www.doiserbia.nb.rs/img/doi/0370-8179/2013/0370-81791304178T.pdf |
work_keys_str_mv |
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