Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa.
Recently, we generated transposon-transgenic boars (Sus scrofa), which carry three monomeric copies of a fluorophore marker gene. Amazingly, a ubiquitous fluorophore expression in somatic, as well as in germ cells was found. Here, we characterized the prominent fluorophore load in mature spermatozoa...
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2011-01-01
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doaj-4580cb0f34b24cce8022bca57e445be02020-11-25T00:04:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01611e2756310.1371/journal.pone.0027563Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa.Wiebke GarrelsStephanie HollerUlrike TaylorDoris HerrmannChristina StruckmannSabine KleinBrigitte Barg-KuesMonika Nowak-ImialekChristine EhlingDetlef RathZoltán IvicsHeiner NiemannWilfried A KuesRecently, we generated transposon-transgenic boars (Sus scrofa), which carry three monomeric copies of a fluorophore marker gene. Amazingly, a ubiquitous fluorophore expression in somatic, as well as in germ cells was found. Here, we characterized the prominent fluorophore load in mature spermatozoa of these animals. Sperm samples were analyzed for general fertility parameters, sorted according to X and Y chromosome-bearing sperm fractions, assessed for potential detrimental effects of the reporter, and used for inseminations into estrous sows. Independent of their genotype, all spermatozoa were uniformly fluorescent with a subcellular compartmentalization of the fluorophore protein in postacrosomal sheath, mid piece and tail. Transmission of the fluorophore protein to fertilized oocytes was shown by confocal microscopic analysis of zygotes. The monomeric copies of the transgene segregated during meiosis, rendering a certain fraction of the spermatozoa non-transgenic (about 10% based on analysis of 74 F1 offspring). The genotype-independent transmission of the fluorophore protein by spermatozoa to oocytes represents a non-genetic contribution to the mammalian embryo.http://europepmc.org/articles/PMC3217978?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wiebke Garrels Stephanie Holler Ulrike Taylor Doris Herrmann Christina Struckmann Sabine Klein Brigitte Barg-Kues Monika Nowak-Imialek Christine Ehling Detlef Rath Zoltán Ivics Heiner Niemann Wilfried A Kues |
spellingShingle |
Wiebke Garrels Stephanie Holler Ulrike Taylor Doris Herrmann Christina Struckmann Sabine Klein Brigitte Barg-Kues Monika Nowak-Imialek Christine Ehling Detlef Rath Zoltán Ivics Heiner Niemann Wilfried A Kues Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa. PLoS ONE |
author_facet |
Wiebke Garrels Stephanie Holler Ulrike Taylor Doris Herrmann Christina Struckmann Sabine Klein Brigitte Barg-Kues Monika Nowak-Imialek Christine Ehling Detlef Rath Zoltán Ivics Heiner Niemann Wilfried A Kues |
author_sort |
Wiebke Garrels |
title |
Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa. |
title_short |
Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa. |
title_full |
Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa. |
title_fullStr |
Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa. |
title_full_unstemmed |
Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa. |
title_sort |
genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
Recently, we generated transposon-transgenic boars (Sus scrofa), which carry three monomeric copies of a fluorophore marker gene. Amazingly, a ubiquitous fluorophore expression in somatic, as well as in germ cells was found. Here, we characterized the prominent fluorophore load in mature spermatozoa of these animals. Sperm samples were analyzed for general fertility parameters, sorted according to X and Y chromosome-bearing sperm fractions, assessed for potential detrimental effects of the reporter, and used for inseminations into estrous sows. Independent of their genotype, all spermatozoa were uniformly fluorescent with a subcellular compartmentalization of the fluorophore protein in postacrosomal sheath, mid piece and tail. Transmission of the fluorophore protein to fertilized oocytes was shown by confocal microscopic analysis of zygotes. The monomeric copies of the transgene segregated during meiosis, rendering a certain fraction of the spermatozoa non-transgenic (about 10% based on analysis of 74 F1 offspring). The genotype-independent transmission of the fluorophore protein by spermatozoa to oocytes represents a non-genetic contribution to the mammalian embryo. |
url |
http://europepmc.org/articles/PMC3217978?pdf=render |
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