The Effect of Polyhydroxyethylmethacrylate (Poly-HEMA), as a Cell Culture Substrate, on Viable and Preserving Nature of Retinal Pigment Epithelium (RPE) Cells
Background: Retinal pigment epithelium (RPE) cells play an important role in the maintenance of normal function of the retina. Tyrosinase is a marker of RPE cells that functions in synthesis of melanin. Polymer of polyhydroxyethylmethacrylate (Poly-HEMA) is the basic component of contact lenses. We...
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doaj-458837e5cc54446fa093071eff0dc8592020-11-25T02:46:31ZfasVesnu Publications مجله دانشکده پزشکی اصفهان1027-75951735-854X2014-11-0132300144414601692The Effect of Polyhydroxyethylmethacrylate (Poly-HEMA), as a Cell Culture Substrate, on Viable and Preserving Nature of Retinal Pigment Epithelium (RPE) CellsFatemeh Nazem-Roaya0Zahra-Soheila Soheili1Razeih Heidari2Abdolkhalegh Deezagi3Hamid Ahmadieh4Shahram Samiei5Abozar Bagheri6National Institute of Genetic Engineering and Biotechnology, Tehran, IranAssistant Professor, National Institute of Genetic Engineering and Biotechnology, Tehran, IranNational Institute of Genetic Engineering and Biotechnology, Tehran, IranAssistant Professor, National Institute of Genetic Engineering and Biotechnology, Tehran, IranAssistant Professor, Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranAssistant Professor, Iranian Blood Transfusion Organization Research Center, Tehran, IranNational Institute of Genetic Engineering and Biotechnology, Tehran, IranBackground: Retinal pigment epithelium (RPE) cells play an important role in the maintenance of normal function of the retina. Tyrosinase is a marker of RPE cells that functions in synthesis of melanin. Polymer of polyhydroxyethylmethacrylate (Poly-HEMA) is the basic component of contact lenses. We investigated the effect of Poly-HEMA, as a cell culture substrate, on viable and preserving nature of RPE cells. Methods: RPE cells between passages 2 and 5 were cultured on Poly-HEMA (12 mg/ml) in 24 wells culture palates. Dulbecco's modified Eagle's medium (DMEM/F12) + 10% Fetal bovine serum (FBS) were used to nourish cultured cells on polystyrene and Poly-HEMA coated vessels. Morphology, the rate of cell proliferation and cell death of RPE cells cultured on Poly-HEMA polymer were evaluated in periodic times. Real-time polymerase chain reaction (RT-PCR) was performed to evaluate tyrosinase in cultured RPE cells. Findings: The cells cultured on Poly-HEMA formed many colonies. These colonies could be re-cultured on polystyrene. The number of RPE cells on Poly-HEMA and polystyrene were the same while the proliferation rate of the cultured cells on Poly-HEMA was reduced; no remarkable cell death was detected on polymer and on polystyrene plates. The giant colonies which were formed on Poly-HEMA were re-cultured. The expression of tyrosinase gene was detected 434.65 in cultured RPE cells on the polymer. Conclusion: Poly-HEMA is a hydrophobic polymer. When RPE cells were cultured on Poly-HEMA they could not adhere to substrate and formed many colonies. Based on our study, Poly-HEMA did not induce cell death in RPE cells but the proliferation of cells was reduced. Tyrosinase expression revealed that Poly-HEMA could support RPE cultures to establish their population as the main constituents of the giant colonies.http://jims.mui.ac.ir/index.php/jims/article/view/4093RPE cellPolymer of Poly-HEMATyrosinase |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Fatemeh Nazem-Roaya Zahra-Soheila Soheili Razeih Heidari Abdolkhalegh Deezagi Hamid Ahmadieh Shahram Samiei Abozar Bagheri |
spellingShingle |
Fatemeh Nazem-Roaya Zahra-Soheila Soheili Razeih Heidari Abdolkhalegh Deezagi Hamid Ahmadieh Shahram Samiei Abozar Bagheri The Effect of Polyhydroxyethylmethacrylate (Poly-HEMA), as a Cell Culture Substrate, on Viable and Preserving Nature of Retinal Pigment Epithelium (RPE) Cells مجله دانشکده پزشکی اصفهان RPE cell Polymer of Poly-HEMA Tyrosinase |
author_facet |
Fatemeh Nazem-Roaya Zahra-Soheila Soheili Razeih Heidari Abdolkhalegh Deezagi Hamid Ahmadieh Shahram Samiei Abozar Bagheri |
author_sort |
Fatemeh Nazem-Roaya |
title |
The Effect of Polyhydroxyethylmethacrylate (Poly-HEMA), as a Cell Culture Substrate, on Viable and Preserving Nature of Retinal Pigment Epithelium (RPE) Cells |
title_short |
The Effect of Polyhydroxyethylmethacrylate (Poly-HEMA), as a Cell Culture Substrate, on Viable and Preserving Nature of Retinal Pigment Epithelium (RPE) Cells |
title_full |
The Effect of Polyhydroxyethylmethacrylate (Poly-HEMA), as a Cell Culture Substrate, on Viable and Preserving Nature of Retinal Pigment Epithelium (RPE) Cells |
title_fullStr |
The Effect of Polyhydroxyethylmethacrylate (Poly-HEMA), as a Cell Culture Substrate, on Viable and Preserving Nature of Retinal Pigment Epithelium (RPE) Cells |
title_full_unstemmed |
The Effect of Polyhydroxyethylmethacrylate (Poly-HEMA), as a Cell Culture Substrate, on Viable and Preserving Nature of Retinal Pigment Epithelium (RPE) Cells |
title_sort |
effect of polyhydroxyethylmethacrylate (poly-hema), as a cell culture substrate, on viable and preserving nature of retinal pigment epithelium (rpe) cells |
publisher |
Vesnu Publications |
series |
مجله دانشکده پزشکی اصفهان |
issn |
1027-7595 1735-854X |
publishDate |
2014-11-01 |
description |
Background: Retinal pigment epithelium (RPE) cells play an important role in the maintenance of normal function of the retina. Tyrosinase is a marker of RPE cells that functions in synthesis of melanin. Polymer of polyhydroxyethylmethacrylate (Poly-HEMA) is the basic component of contact lenses. We investigated the effect of Poly-HEMA, as a cell culture substrate, on viable and preserving nature of RPE cells.
Methods: RPE cells between passages 2 and 5 were cultured on Poly-HEMA (12 mg/ml) in 24 wells culture palates. Dulbecco's modified Eagle's medium (DMEM/F12) + 10% Fetal bovine serum (FBS) were used to nourish cultured cells on polystyrene and Poly-HEMA coated vessels. Morphology, the rate of cell proliferation and cell death of RPE cells cultured on Poly-HEMA polymer were evaluated in periodic times. Real-time polymerase chain reaction (RT-PCR) was performed to evaluate tyrosinase in cultured RPE cells.
Findings: The cells cultured on Poly-HEMA formed many colonies. These colonies could be re-cultured on polystyrene. The number of RPE cells on Poly-HEMA and polystyrene were the same while the proliferation rate of the cultured cells on Poly-HEMA was reduced; no remarkable cell death was detected on polymer and on polystyrene plates. The giant colonies which were formed on Poly-HEMA were re-cultured. The expression of tyrosinase gene was detected 434.65 in cultured RPE cells on the polymer.
Conclusion: Poly-HEMA is a hydrophobic polymer. When RPE cells were cultured on Poly-HEMA they could not adhere to substrate and formed many colonies. Based on our study, Poly-HEMA did not induce cell death in RPE cells but the proliferation of cells was reduced. Tyrosinase expression revealed that Poly-HEMA could support RPE cultures to establish their population as the main constituents of the giant colonies. |
topic |
RPE cell Polymer of Poly-HEMA Tyrosinase |
url |
http://jims.mui.ac.ir/index.php/jims/article/view/4093 |
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