Flavonoids, cytotoxic, antioxidant and antibacterial activities of Evax pygmaea
Context: Phytochemical study and biological potential of Evax pygmaea (L.) Brot. (Asteraceae) are reported for the first time. Objective: To identify the secondary metabolites of Evax pygmaea and to determine its antioxidant, antibacterial and cytotoxic activities. Materials and methods: Dried aeria...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2017-01-01
|
Series: | Pharmaceutical Biology |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/13880209.2017.1405997 |
id |
doaj-4589a974c5d94c02a453e2fc180a11b4 |
---|---|
record_format |
Article |
spelling |
doaj-4589a974c5d94c02a453e2fc180a11b42020-11-25T03:11:29ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162017-01-015512292229610.1080/13880209.2017.14059971405997Flavonoids, cytotoxic, antioxidant and antibacterial activities of Evax pygmaeaAssia Khalfallah0Djemaa Berrehal1Chawki Bensouici2Ahmed Kabouche3Zahia Semra4Laurence Voutquenne-Nazabadioko5Abdulmagid Alabdul Magid6Zahia Kabouche7Université des frères Mentouri-ConstantineUniversité des frères Mentouri-ConstantineCentre de Recherche en BiotechnologieUniversité des frères Mentouri-ConstantineUniversité des frères Mentouri-ConstantineInstitut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312Institut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312Université des frères Mentouri-ConstantineContext: Phytochemical study and biological potential of Evax pygmaea (L.) Brot. (Asteraceae) are reported for the first time. Objective: To identify the secondary metabolites of Evax pygmaea and to determine its antioxidant, antibacterial and cytotoxic activities. Materials and methods: Dried aerial parts (1 kg) were macerated in 70% MeOH (5 L) during 72 h. The concentrated hydromethanolic extract was subjected to extractions with chloroform (3 × 300 mL), ethyl acetate (3 × 300 mL) and n-butanol (3 × 300 mL), successively. VLC of combined ethyl acetate (EAEP) and n-butanol (BEP) fractions was followed by column purifications. Antioxidant activity was investigated using DPPH, CUPRAC, and metal chelating, β-carotene/linoleic acid and ABTS assays. Agar method was used in the antibacterial study. Cytotoxic activity was determined by Brine shrimp lethality test in DMSO and ethanol, at varying concentrations (2, 1 and 0.2%) and (1, 0.2 and 0.1%) successively. Results: Quercetin (1), isorhamnetin 3-O-β-d-xyloside (2), isorhamnetin 3-O-β-d-glucoside (3), quercetin 3-O-β-d-glucoside (4), quercetin 7-O-β-D-glucoside (5), patuletin 3-O-β-d-glucoside (6) were isolated from for the first time from Evax genus. The EAEP was the most active in ABTS (IC50: <3.125 μg/mL) assay whereas the BEEP exhibited the highest activity in the β-carotene/linoleic acid assay (IC50: <3.125 μg/mL). The EAEP and BEP exhibited good antibacterial activity (MIC: 40–80 µg/mL). The plant did not show any toxicity (LD50>80 µg/mL). Discussion and conclusions: Six flavonoids were isolated for the first time from Evax pygmaea which exhibited good antioxidant and antibacterial activities.http://dx.doi.org/10.1080/13880209.2017.1405997dpphcupracmetal chelatingβ-carotene/linoleic acidabtsmic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Assia Khalfallah Djemaa Berrehal Chawki Bensouici Ahmed Kabouche Zahia Semra Laurence Voutquenne-Nazabadioko Abdulmagid Alabdul Magid Zahia Kabouche |
spellingShingle |
Assia Khalfallah Djemaa Berrehal Chawki Bensouici Ahmed Kabouche Zahia Semra Laurence Voutquenne-Nazabadioko Abdulmagid Alabdul Magid Zahia Kabouche Flavonoids, cytotoxic, antioxidant and antibacterial activities of Evax pygmaea Pharmaceutical Biology dpph cuprac metal chelating β-carotene/linoleic acid abts mic |
author_facet |
Assia Khalfallah Djemaa Berrehal Chawki Bensouici Ahmed Kabouche Zahia Semra Laurence Voutquenne-Nazabadioko Abdulmagid Alabdul Magid Zahia Kabouche |
author_sort |
Assia Khalfallah |
title |
Flavonoids, cytotoxic, antioxidant and antibacterial activities of Evax pygmaea |
title_short |
Flavonoids, cytotoxic, antioxidant and antibacterial activities of Evax pygmaea |
title_full |
Flavonoids, cytotoxic, antioxidant and antibacterial activities of Evax pygmaea |
title_fullStr |
Flavonoids, cytotoxic, antioxidant and antibacterial activities of Evax pygmaea |
title_full_unstemmed |
Flavonoids, cytotoxic, antioxidant and antibacterial activities of Evax pygmaea |
title_sort |
flavonoids, cytotoxic, antioxidant and antibacterial activities of evax pygmaea |
publisher |
Taylor & Francis Group |
series |
Pharmaceutical Biology |
issn |
1388-0209 1744-5116 |
publishDate |
2017-01-01 |
description |
Context: Phytochemical study and biological potential of Evax pygmaea (L.) Brot. (Asteraceae) are reported for the first time. Objective: To identify the secondary metabolites of Evax pygmaea and to determine its antioxidant, antibacterial and cytotoxic activities. Materials and methods: Dried aerial parts (1 kg) were macerated in 70% MeOH (5 L) during 72 h. The concentrated hydromethanolic extract was subjected to extractions with chloroform (3 × 300 mL), ethyl acetate (3 × 300 mL) and n-butanol (3 × 300 mL), successively. VLC of combined ethyl acetate (EAEP) and n-butanol (BEP) fractions was followed by column purifications. Antioxidant activity was investigated using DPPH, CUPRAC, and metal chelating, β-carotene/linoleic acid and ABTS assays. Agar method was used in the antibacterial study. Cytotoxic activity was determined by Brine shrimp lethality test in DMSO and ethanol, at varying concentrations (2, 1 and 0.2%) and (1, 0.2 and 0.1%) successively. Results: Quercetin (1), isorhamnetin 3-O-β-d-xyloside (2), isorhamnetin 3-O-β-d-glucoside (3), quercetin 3-O-β-d-glucoside (4), quercetin 7-O-β-D-glucoside (5), patuletin 3-O-β-d-glucoside (6) were isolated from for the first time from Evax genus. The EAEP was the most active in ABTS (IC50: <3.125 μg/mL) assay whereas the BEEP exhibited the highest activity in the β-carotene/linoleic acid assay (IC50: <3.125 μg/mL). The EAEP and BEP exhibited good antibacterial activity (MIC: 40–80 µg/mL). The plant did not show any toxicity (LD50>80 µg/mL). Discussion and conclusions: Six flavonoids were isolated for the first time from Evax pygmaea which exhibited good antioxidant and antibacterial activities. |
topic |
dpph cuprac metal chelating β-carotene/linoleic acid abts mic |
url |
http://dx.doi.org/10.1080/13880209.2017.1405997 |
work_keys_str_mv |
AT assiakhalfallah flavonoidscytotoxicantioxidantandantibacterialactivitiesofevaxpygmaea AT djemaaberrehal flavonoidscytotoxicantioxidantandantibacterialactivitiesofevaxpygmaea AT chawkibensouici flavonoidscytotoxicantioxidantandantibacterialactivitiesofevaxpygmaea AT ahmedkabouche flavonoidscytotoxicantioxidantandantibacterialactivitiesofevaxpygmaea AT zahiasemra flavonoidscytotoxicantioxidantandantibacterialactivitiesofevaxpygmaea AT laurencevoutquennenazabadioko flavonoidscytotoxicantioxidantandantibacterialactivitiesofevaxpygmaea AT abdulmagidalabdulmagid flavonoidscytotoxicantioxidantandantibacterialactivitiesofevaxpygmaea AT zahiakabouche flavonoidscytotoxicantioxidantandantibacterialactivitiesofevaxpygmaea |
_version_ |
1724653867514200064 |