Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes
Sulforaphane, the hydrolytic product of glucoraphanin glucosinolate, is a potent anticarcinogen that reduces the risk of several human cancers. However, in most B. rapa vegetables, glucoraphanin is undetectable or only present in trace amounts, since the glucoraphanin that is present is converted to...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2017-08-01
|
Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01329/full |
id |
doaj-458e7ad56ed44e2282eebe2868d34675 |
---|---|
record_format |
Article |
spelling |
doaj-458e7ad56ed44e2282eebe2868d346752020-11-24T22:54:35ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-08-01810.3389/fpls.2017.01329277846Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional GenesZhiyuan Liu0Zhiyuan Liu1Jianli Liang2Shuning Zheng3Jifang Zhang4Jian Wu5Feng Cheng6Wencai Yang7Xiaowu Wang8Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesBeijing, ChinaCollege of Horticulture, China Agricultural UniversityBeijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesBeijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesBeijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesBeijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesBeijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesBeijing, ChinaCollege of Horticulture, China Agricultural UniversityBeijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesBeijing, ChinaSulforaphane, the hydrolytic product of glucoraphanin glucosinolate, is a potent anticarcinogen that reduces the risk of several human cancers. However, in most B. rapa vegetables, glucoraphanin is undetectable or only present in trace amounts, since the glucoraphanin that is present is converted to gluconapin by three functional BrAOP2 genes. In this study, to enrich beneficial glucoraphanin content in B. rapa, the functional BrAOP2 alleles were replaced by non-functional counterparts through marker-assisted backcrossing (MAB). We identified non-functional mutations of two BrAOP2 genes from B. rapa. The backcross progenies with introgression of both non-functional braop2.2 and braop2.3 alleles significantly increased the glucoraphanin content by 18 times relative to the recurrent parent. In contrast, replacement or introgression of single non-functional braop2.2 or braop2.3 locus did not change glucoraphanin content. Our results suggest that replacement of these two functional BrAOP2 genes with non-functional alleles has the potential for producing improved Brassica crops with enriched beneficial glucoraphanin content.http://journal.frontiersin.org/article/10.3389/fpls.2017.01329/fullBrassica rapaglucosinolateglucoraphaninBrAOP2Marker-assisted backcrossing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhiyuan Liu Zhiyuan Liu Jianli Liang Shuning Zheng Jifang Zhang Jian Wu Feng Cheng Wencai Yang Xiaowu Wang |
spellingShingle |
Zhiyuan Liu Zhiyuan Liu Jianli Liang Shuning Zheng Jifang Zhang Jian Wu Feng Cheng Wencai Yang Xiaowu Wang Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes Frontiers in Plant Science Brassica rapa glucosinolate glucoraphanin BrAOP2 Marker-assisted backcrossing |
author_facet |
Zhiyuan Liu Zhiyuan Liu Jianli Liang Shuning Zheng Jifang Zhang Jian Wu Feng Cheng Wencai Yang Xiaowu Wang |
author_sort |
Zhiyuan Liu |
title |
Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes |
title_short |
Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes |
title_full |
Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes |
title_fullStr |
Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes |
title_full_unstemmed |
Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes |
title_sort |
enriching glucoraphanin in brassica rapa through replacement of braop2.2/braop2.3 with non-functional genes |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2017-08-01 |
description |
Sulforaphane, the hydrolytic product of glucoraphanin glucosinolate, is a potent anticarcinogen that reduces the risk of several human cancers. However, in most B. rapa vegetables, glucoraphanin is undetectable or only present in trace amounts, since the glucoraphanin that is present is converted to gluconapin by three functional BrAOP2 genes. In this study, to enrich beneficial glucoraphanin content in B. rapa, the functional BrAOP2 alleles were replaced by non-functional counterparts through marker-assisted backcrossing (MAB). We identified non-functional mutations of two BrAOP2 genes from B. rapa. The backcross progenies with introgression of both non-functional braop2.2 and braop2.3 alleles significantly increased the glucoraphanin content by 18 times relative to the recurrent parent. In contrast, replacement or introgression of single non-functional braop2.2 or braop2.3 locus did not change glucoraphanin content. Our results suggest that replacement of these two functional BrAOP2 genes with non-functional alleles has the potential for producing improved Brassica crops with enriched beneficial glucoraphanin content. |
topic |
Brassica rapa glucosinolate glucoraphanin BrAOP2 Marker-assisted backcrossing |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2017.01329/full |
work_keys_str_mv |
AT zhiyuanliu enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes AT zhiyuanliu enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes AT jianliliang enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes AT shuningzheng enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes AT jifangzhang enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes AT jianwu enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes AT fengcheng enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes AT wencaiyang enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes AT xiaowuwang enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes |
_version_ |
1725658866887163904 |