Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation
Aseptic loosening and periprosthetic osteolysis are the leading causes of total joint arthroplasty failure, which occurs as a result of chronic inflammatory response and enhanced osteoclast activity. Here we showed that stevioside, a natural compound isolated from Stevia rebaudiana, exhibited preven...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-09-01
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| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2018.01053/full |
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doaj-459ace042c8b4823be44850161017026 |
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| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Jiahong Meng Jiahong Meng Chenhe Zhou Chenhe Zhou Bin Hu Bin Hu Mengmeng Luo Yute Yang Yute Yang Yangxin Wang Yangxin Wang Wei Wang Wei Wang Guangyao Jiang Guangyao Jiang Jianqiao Hong Jianqiao Hong Sihao Li Sihao Li Haobo Wu Haobo Wu Shigui Yan Shigui Yan Weiqi Yan Weiqi Yan |
| spellingShingle |
Jiahong Meng Jiahong Meng Chenhe Zhou Chenhe Zhou Bin Hu Bin Hu Mengmeng Luo Yute Yang Yute Yang Yangxin Wang Yangxin Wang Wei Wang Wei Wang Guangyao Jiang Guangyao Jiang Jianqiao Hong Jianqiao Hong Sihao Li Sihao Li Haobo Wu Haobo Wu Shigui Yan Shigui Yan Weiqi Yan Weiqi Yan Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation Frontiers in Pharmacology aseptic loosening osteoclast NF-κB – nuclear factor-kappa B MAPK TAK1 |
| author_facet |
Jiahong Meng Jiahong Meng Chenhe Zhou Chenhe Zhou Bin Hu Bin Hu Mengmeng Luo Yute Yang Yute Yang Yangxin Wang Yangxin Wang Wei Wang Wei Wang Guangyao Jiang Guangyao Jiang Jianqiao Hong Jianqiao Hong Sihao Li Sihao Li Haobo Wu Haobo Wu Shigui Yan Shigui Yan Weiqi Yan Weiqi Yan |
| author_sort |
Jiahong Meng |
| title |
Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation |
| title_short |
Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation |
| title_full |
Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation |
| title_fullStr |
Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation |
| title_full_unstemmed |
Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation |
| title_sort |
stevioside prevents wear particle-induced osteolysis by inhibiting osteoclastogenesis and inflammatory response via the suppression of tak1 activation |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Pharmacology |
| issn |
1663-9812 |
| publishDate |
2018-09-01 |
| description |
Aseptic loosening and periprosthetic osteolysis are the leading causes of total joint arthroplasty failure, which occurs as a result of chronic inflammatory response and enhanced osteoclast activity. Here we showed that stevioside, a natural compound isolated from Stevia rebaudiana, exhibited preventative effects on titanium particle-induced osteolysis in a mouse calvarial model. Further histological assessment and real-time PCR analysis indicated that stevioside prevented titanium particle-induced osteolysis by inhibiting osteoclast formation and inflammatory cytokine expression in vivo. In vitro, we found that stevioside could suppress RANKL-induced osteoclastogenesis and titanium particle-induced inflammatory response in a dose-dependent manner. Mechanistically, stevioside achieved these effects by disrupting the phosphorylation of TAK1 and subsequent activation of NF-κB/MAPKs signaling pathways. Collectively, our data suggest that stevioside effectively suppresses osteoclastogenesis and inflammatory response both in vitro and in vivo, and it might be a potential therapy for particle-induced osteolysis and other osteolytic diseases. |
| topic |
aseptic loosening osteoclast NF-κB – nuclear factor-kappa B MAPK TAK1 |
| url |
https://www.frontiersin.org/article/10.3389/fphar.2018.01053/full |
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doaj-459ace042c8b4823be448501610170262020-11-25T01:56:31ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-09-01910.3389/fphar.2018.01053410352Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 ActivationJiahong Meng0Jiahong Meng1Chenhe Zhou2Chenhe Zhou3Bin Hu4Bin Hu5Mengmeng Luo6Yute Yang7Yute Yang8Yangxin Wang9Yangxin Wang10Wei Wang11Wei Wang12Guangyao Jiang13Guangyao Jiang14Jianqiao Hong15Jianqiao Hong16Sihao Li17Sihao Li18Haobo Wu19Haobo Wu20Shigui Yan21Shigui Yan22Weiqi Yan23Weiqi Yan24Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaOrthopedic Research Institute of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaOrthopedic Research Institute of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaOrthopedic Research Institute of Zhejiang University, Hangzhou, ChinaDepartment of Plastic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaOrthopedic Research Institute of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaOrthopedic Research Institute of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaOrthopedic Research Institute of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaOrthopedic Research Institute of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaOrthopedic Research Institute of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaOrthopedic Research Institute of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaOrthopedic Research Institute of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaOrthopedic Research Institute of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaOrthopedic Research Institute of Zhejiang University, Hangzhou, ChinaAseptic loosening and periprosthetic osteolysis are the leading causes of total joint arthroplasty failure, which occurs as a result of chronic inflammatory response and enhanced osteoclast activity. Here we showed that stevioside, a natural compound isolated from Stevia rebaudiana, exhibited preventative effects on titanium particle-induced osteolysis in a mouse calvarial model. Further histological assessment and real-time PCR analysis indicated that stevioside prevented titanium particle-induced osteolysis by inhibiting osteoclast formation and inflammatory cytokine expression in vivo. In vitro, we found that stevioside could suppress RANKL-induced osteoclastogenesis and titanium particle-induced inflammatory response in a dose-dependent manner. Mechanistically, stevioside achieved these effects by disrupting the phosphorylation of TAK1 and subsequent activation of NF-κB/MAPKs signaling pathways. Collectively, our data suggest that stevioside effectively suppresses osteoclastogenesis and inflammatory response both in vitro and in vivo, and it might be a potential therapy for particle-induced osteolysis and other osteolytic diseases.https://www.frontiersin.org/article/10.3389/fphar.2018.01053/fullaseptic looseningosteoclastNF-κB – nuclear factor-kappa BMAPKTAK1 |