Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model
The aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP)...
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doaj-45a04311df6341c7a2e09a07e948f1912020-11-24T21:25:10ZengMDPI AGMaterials1996-19442019-05-011210161310.3390/ma12101613ma12101613Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial ModelEunhye Jang0Ja-Youn Lee1Eun-Young Lee2Hyun Seok3Department of Orthodontics, College of Dentistry, Gangneung-Wonju National University, Gangneung 25457, KoreaDepartment of Prosthodontics, Chungbuk National University Hospital, Cheongju 28644, KoreaDepartment of Oral and Maxillofacial Surgery, Chungbuk National University College of Medicine, Cheongju 28644, KoreaDepartment of Oral and Maxillofacial Surgery, Chungbuk National University Hospital, Cheongju 28644, KoreaThe aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP) (5 µg), and ABB/high BMP (50 µg) were grafted as subperiosteal bone grafts. The new bone formation parameters of bone volume (BV), bone mineral density (BMD), trabecular thickness (TbTh), and trabecular spacing (TbSp) were evaluated by microcomputed tomography (µ-CT), and a histomorphometric analysis was performed to evaluate the new bone formation area. The expression of osteogenic markers, such as bone sialoprotein (BSP) and osteocalcin, were evaluated by immunohistochemistry (IHC). The ABB/high BMP group showed significantly higher BV than the ABB/low BMP (<i>p</i> = 0.004) and control groups (<i>p</i> = 0.000) and higher TbTh than the control group (<i>p</i> = 0.000). The ABB/low BMP group showed significantly higher BV, BMD, and TbTh than the control group (<i>p</i> = 0.002, 0.042, and 0.000, respectively). The histomorphometry showed significantly higher bone formation in the ABB/low and high BMP groups than in the control group (<i>p</i> = 0.000). IHC showed a high expression of BSP and osteocalcin in the ABB/low and high BMP groups. Subperiosteal bone grafts with ABB and rhBMP-2 have not been studied. In our study, we confirmed that rhBMP-2 contributes to new bone formation in a subperiosteal bone graft with ABB.https://www.mdpi.com/1996-1944/12/10/1613bone morphogenetic proteinbone regenerationsubperiosteal bone graftbone sialoproteinosteocalcin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eunhye Jang Ja-Youn Lee Eun-Young Lee Hyun Seok |
spellingShingle |
Eunhye Jang Ja-Youn Lee Eun-Young Lee Hyun Seok Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model Materials bone morphogenetic protein bone regeneration subperiosteal bone graft bone sialoprotein osteocalcin |
author_facet |
Eunhye Jang Ja-Youn Lee Eun-Young Lee Hyun Seok |
author_sort |
Eunhye Jang |
title |
Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model |
title_short |
Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model |
title_full |
Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model |
title_fullStr |
Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model |
title_full_unstemmed |
Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model |
title_sort |
evaluation of the bone regeneration effect of recombinant human bone morphogenic protein-2 on subperiosteal bone graft in the rat calvarial model |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2019-05-01 |
description |
The aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP) (5 µg), and ABB/high BMP (50 µg) were grafted as subperiosteal bone grafts. The new bone formation parameters of bone volume (BV), bone mineral density (BMD), trabecular thickness (TbTh), and trabecular spacing (TbSp) were evaluated by microcomputed tomography (µ-CT), and a histomorphometric analysis was performed to evaluate the new bone formation area. The expression of osteogenic markers, such as bone sialoprotein (BSP) and osteocalcin, were evaluated by immunohistochemistry (IHC). The ABB/high BMP group showed significantly higher BV than the ABB/low BMP (<i>p</i> = 0.004) and control groups (<i>p</i> = 0.000) and higher TbTh than the control group (<i>p</i> = 0.000). The ABB/low BMP group showed significantly higher BV, BMD, and TbTh than the control group (<i>p</i> = 0.002, 0.042, and 0.000, respectively). The histomorphometry showed significantly higher bone formation in the ABB/low and high BMP groups than in the control group (<i>p</i> = 0.000). IHC showed a high expression of BSP and osteocalcin in the ABB/low and high BMP groups. Subperiosteal bone grafts with ABB and rhBMP-2 have not been studied. In our study, we confirmed that rhBMP-2 contributes to new bone formation in a subperiosteal bone graft with ABB. |
topic |
bone morphogenetic protein bone regeneration subperiosteal bone graft bone sialoprotein osteocalcin |
url |
https://www.mdpi.com/1996-1944/12/10/1613 |
work_keys_str_mv |
AT eunhyejang evaluationoftheboneregenerationeffectofrecombinanthumanbonemorphogenicprotein2onsubperiostealbonegraftintheratcalvarialmodel AT jayounlee evaluationoftheboneregenerationeffectofrecombinanthumanbonemorphogenicprotein2onsubperiostealbonegraftintheratcalvarialmodel AT eunyounglee evaluationoftheboneregenerationeffectofrecombinanthumanbonemorphogenicprotein2onsubperiostealbonegraftintheratcalvarialmodel AT hyunseok evaluationoftheboneregenerationeffectofrecombinanthumanbonemorphogenicprotein2onsubperiostealbonegraftintheratcalvarialmodel |
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1725984336243589120 |