High Interleukin-12 Levels May Prevent an Increase in the Amount of Fungi in the Gastrointestinal Tract during the First Years of Diabetes Mellitus Type 1
The objective of the research was to investigate serum levels of interleukin-12 (IL12) in relation to percentage of yeast-like fungi colonies residing in the gastrointestinal tract in children and adolescents with type 1 diabetes mellitus (T1DM). The study involved 83 children and adolescents, inclu...
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doaj-45c0807f3a3b43a6ba42d2703ca9ba762020-11-25T00:37:38ZengHindawi LimitedDisease Markers0278-02401875-86302016-01-01201610.1155/2016/46859764685976High Interleukin-12 Levels May Prevent an Increase in the Amount of Fungi in the Gastrointestinal Tract during the First Years of Diabetes Mellitus Type 1Beata Kowalewska0Katarzyna Zorena1Małgorzata Szmigiero-Kawko2Piotr Wąż3Małgorzata Myśliwiec4Department of Tropical Medicine and Epidemiology, Institute of Maritime and Tropical Medicine, Medical University of Gdańsk, Gdańsk, PolandDepartment of Immunobiology and Environment Microbiology, Medical University of Gdańsk, Gdańsk, PolandClinics of Paediatrics, Diabetology and Endocrinology, Medical University of Gdańsk, Gdańsk, PolandDepartment of Nuclear Medicine, Medical University of Gdańsk, Gdańsk, PolandClinics of Paediatrics, Diabetology and Endocrinology, Medical University of Gdańsk, Gdańsk, PolandThe objective of the research was to investigate serum levels of interleukin-12 (IL12) in relation to percentage of yeast-like fungi colonies residing in the gastrointestinal tract in children and adolescents with type 1 diabetes mellitus (T1DM). The study involved 83 children and adolescents, including 53 T1DM patients and 30 healthy control subjects. In the studied population biochemical tests were performed and yeast-like fungi were identified in the faeces. Moreover, IL12 absorbance was measured and measurements of Candida albicans IgG and IgM antibodies were performed with microplate reader ChroMate 4300 (Awareness Technology, Inc., USA) at wavelength λ=450 nm. In the group of T1DM children and adolescents with disease duration ≤ 2 years, high levels of IL12 were found with lower percentage of yeast-like fungal colonies versus T1DM patients with disease duration > 2 years and ≤5 years, as well as versus T1DM patients with disease duration > 5 years. Additionally, serum levels of IL12 were found to be decreasing by 18.1 pg/ml with each year of diabetes duration. IL12 serum levels were also found to be decreasing by 52.9 pg/ml with each 1% increase in HbA1c. We suggest that high IL12 levels can inhibit infection with yeast-like fungi colonizing the gastrointestinal tract in children and adolescents with T1DM. Further studies are needed to confirm the antifungal activity of IL12.http://dx.doi.org/10.1155/2016/4685976 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Beata Kowalewska Katarzyna Zorena Małgorzata Szmigiero-Kawko Piotr Wąż Małgorzata Myśliwiec |
spellingShingle |
Beata Kowalewska Katarzyna Zorena Małgorzata Szmigiero-Kawko Piotr Wąż Małgorzata Myśliwiec High Interleukin-12 Levels May Prevent an Increase in the Amount of Fungi in the Gastrointestinal Tract during the First Years of Diabetes Mellitus Type 1 Disease Markers |
author_facet |
Beata Kowalewska Katarzyna Zorena Małgorzata Szmigiero-Kawko Piotr Wąż Małgorzata Myśliwiec |
author_sort |
Beata Kowalewska |
title |
High Interleukin-12 Levels May Prevent an Increase in the Amount of Fungi in the Gastrointestinal Tract during the First Years of Diabetes Mellitus Type 1 |
title_short |
High Interleukin-12 Levels May Prevent an Increase in the Amount of Fungi in the Gastrointestinal Tract during the First Years of Diabetes Mellitus Type 1 |
title_full |
High Interleukin-12 Levels May Prevent an Increase in the Amount of Fungi in the Gastrointestinal Tract during the First Years of Diabetes Mellitus Type 1 |
title_fullStr |
High Interleukin-12 Levels May Prevent an Increase in the Amount of Fungi in the Gastrointestinal Tract during the First Years of Diabetes Mellitus Type 1 |
title_full_unstemmed |
High Interleukin-12 Levels May Prevent an Increase in the Amount of Fungi in the Gastrointestinal Tract during the First Years of Diabetes Mellitus Type 1 |
title_sort |
high interleukin-12 levels may prevent an increase in the amount of fungi in the gastrointestinal tract during the first years of diabetes mellitus type 1 |
publisher |
Hindawi Limited |
series |
Disease Markers |
issn |
0278-0240 1875-8630 |
publishDate |
2016-01-01 |
description |
The objective of the research was to investigate serum levels of interleukin-12 (IL12) in relation to percentage of yeast-like fungi colonies residing in the gastrointestinal tract in children and adolescents with type 1 diabetes mellitus (T1DM). The study involved 83 children and adolescents, including 53 T1DM patients and 30 healthy control subjects. In the studied population biochemical tests were performed and yeast-like fungi were identified in the faeces. Moreover, IL12 absorbance was measured and measurements of Candida albicans IgG and IgM antibodies were performed with microplate reader ChroMate 4300 (Awareness Technology, Inc., USA) at wavelength λ=450 nm. In the group of T1DM children and adolescents with disease duration ≤ 2 years, high levels of IL12 were found with lower percentage of yeast-like fungal colonies versus T1DM patients with disease duration > 2 years and ≤5 years, as well as versus T1DM patients with disease duration > 5 years. Additionally, serum levels of IL12 were found to be decreasing by 18.1 pg/ml with each year of diabetes duration. IL12 serum levels were also found to be decreasing by 52.9 pg/ml with each 1% increase in HbA1c. We suggest that high IL12 levels can inhibit infection with yeast-like fungi colonizing the gastrointestinal tract in children and adolescents with T1DM. Further studies are needed to confirm the antifungal activity of IL12. |
url |
http://dx.doi.org/10.1155/2016/4685976 |
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