Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus)
The reproductive characteristics and ovarian development in cotton rats (Sigmodon hispidus, CRs) are unclear, although CRs are commonly used as animal models in biomedical research. We previously reported that young (6–8 weeks) CRs showed multi-oocyte follicles (MOFs) and double nucleated oocytes (D...
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Frontiers Media S.A.
2021-01-01
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Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2020.601927/full |
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doaj-45ce3be305a64fa18cc9bd319395e73f |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Md. Rashedul Islam Md. Rashedul Islam Osamu Ichii Osamu Ichii Teppei Nakamura Teppei Nakamura Takao Irie Md. Abdul Masum Md. Abdul Masum Yuki Otani Takashi Namba Tsolmon Chuluunbaatar Tsolmon Chuluunbaatar Yaser Hosny Ali Elewa Yaser Hosny Ali Elewa Yasuhiro Kon |
spellingShingle |
Md. Rashedul Islam Md. Rashedul Islam Osamu Ichii Osamu Ichii Teppei Nakamura Teppei Nakamura Takao Irie Md. Abdul Masum Md. Abdul Masum Yuki Otani Takashi Namba Tsolmon Chuluunbaatar Tsolmon Chuluunbaatar Yaser Hosny Ali Elewa Yaser Hosny Ali Elewa Yasuhiro Kon Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus) Frontiers in Physiology neonatal cotton rat folliculogenesis multi-oocyte follicles double nucleated oocytes apoptosis oocytogenesis |
author_facet |
Md. Rashedul Islam Md. Rashedul Islam Osamu Ichii Osamu Ichii Teppei Nakamura Teppei Nakamura Takao Irie Md. Abdul Masum Md. Abdul Masum Yuki Otani Takashi Namba Tsolmon Chuluunbaatar Tsolmon Chuluunbaatar Yaser Hosny Ali Elewa Yaser Hosny Ali Elewa Yasuhiro Kon |
author_sort |
Md. Rashedul Islam |
title |
Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus) |
title_short |
Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus) |
title_full |
Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus) |
title_fullStr |
Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus) |
title_full_unstemmed |
Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus) |
title_sort |
developmental changes of the ovary in neonatal cotton rat (sigmodon hispidus) |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2021-01-01 |
description |
The reproductive characteristics and ovarian development in cotton rats (Sigmodon hispidus, CRs) are unclear, although CRs are commonly used as animal models in biomedical research. We previously reported that young (6–8 weeks) CRs showed multi-oocyte follicles (MOFs) and double nucleated oocytes (DNOs) in different stages of follicles. The developmental changes in neonatal CR ovaries were investigated in the present study and were compared with our findings in previous studies of unique phenotypes, particularly in oocytes. CR ovaries at postnatal days (PND) 0, 4, and 7 were obtained from the Hokkaido Institute of Public Health. Samples were analyzed by light and transmission electron microscopy. The general histology and folliculogenesis in CR ovaries were similar to those in other experimental rodents. However, DNOs were observed in all age categories and were frequently observed in primordial follicles, whereas MOFs started to develop from PND4 with greater frequency in primary follicles. Almost all developing follicles expressed DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 and forkhead box L2, which are representative markers of oocytes and follicular epithelial cells, respectively. Ki-67 staining demonstrated the proliferative activity of granulosa cells, but not of oocytes, in follicles. Moreover, rapid folliculogenesis of CR due to a small number of apoptotic oocytes was suggested, based on results of the terminal deoxynucleotidyl transferase dUTP nick end labeling assay, confirming the formation of DNOs or MOFs. These findings clarify the development of unique phenotypes of neonatal CR ovaries and support it as a useful model to better understand folliculogenesis and oocytogenesis as well as their abnormalities in humans and other animals. |
topic |
neonatal cotton rat folliculogenesis multi-oocyte follicles double nucleated oocytes apoptosis oocytogenesis |
url |
https://www.frontiersin.org/articles/10.3389/fphys.2020.601927/full |
work_keys_str_mv |
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doaj-45ce3be305a64fa18cc9bd319395e73f2021-01-13T05:50:48ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-01-011110.3389/fphys.2020.601927601927Developmental Changes of the Ovary in Neonatal Cotton Rat (Sigmodon hispidus)Md. Rashedul Islam0Md. Rashedul Islam1Osamu Ichii2Osamu Ichii3Teppei Nakamura4Teppei Nakamura5Takao Irie6Md. Abdul Masum7Md. Abdul Masum8Yuki Otani9Takashi Namba10Tsolmon Chuluunbaatar11Tsolmon Chuluunbaatar12Yaser Hosny Ali Elewa13Yaser Hosny Ali Elewa14Yasuhiro Kon15Laboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Surgery and Theriogenology, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka, BangladeshLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanLaboratory of Agrobiomedical Science, Faculty of Agriculture, Hokkaido University, Sapporo, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanSection of Biological Safety Research, Chitose Laboratory, Japan Food Research Laboratories, Chitose, JapanLaboratory of Veterinary Parasitology, Faculty of Agriculture, University of Miyazaki, Miyazaki, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Anatomy, Histology and Physiology, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka, BangladeshLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Basic Science of Veterinary Medicine, School of Veterinary Medicine, Mongolian University of Life Science, Ulaanbaatar, MongoliaLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Histology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, EgyptLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanThe reproductive characteristics and ovarian development in cotton rats (Sigmodon hispidus, CRs) are unclear, although CRs are commonly used as animal models in biomedical research. We previously reported that young (6–8 weeks) CRs showed multi-oocyte follicles (MOFs) and double nucleated oocytes (DNOs) in different stages of follicles. The developmental changes in neonatal CR ovaries were investigated in the present study and were compared with our findings in previous studies of unique phenotypes, particularly in oocytes. CR ovaries at postnatal days (PND) 0, 4, and 7 were obtained from the Hokkaido Institute of Public Health. Samples were analyzed by light and transmission electron microscopy. The general histology and folliculogenesis in CR ovaries were similar to those in other experimental rodents. However, DNOs were observed in all age categories and were frequently observed in primordial follicles, whereas MOFs started to develop from PND4 with greater frequency in primary follicles. Almost all developing follicles expressed DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 and forkhead box L2, which are representative markers of oocytes and follicular epithelial cells, respectively. Ki-67 staining demonstrated the proliferative activity of granulosa cells, but not of oocytes, in follicles. Moreover, rapid folliculogenesis of CR due to a small number of apoptotic oocytes was suggested, based on results of the terminal deoxynucleotidyl transferase dUTP nick end labeling assay, confirming the formation of DNOs or MOFs. These findings clarify the development of unique phenotypes of neonatal CR ovaries and support it as a useful model to better understand folliculogenesis and oocytogenesis as well as their abnormalities in humans and other animals.https://www.frontiersin.org/articles/10.3389/fphys.2020.601927/fullneonatal cotton ratfolliculogenesismulti-oocyte folliclesdouble nucleated oocytesapoptosisoocytogenesis |