Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia
The objective of this work was to evaluate the catabolic gene diversity for the bacterial degradation of aromatic hydrocarbons in anthropogenic dark earth of Amazonia (ADE) and their biochar (BC). Functional diversity analyses in ADE soils can provide information on how adaptive microorganisms may i...
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2012-05-01
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doaj-45ee49f692b54ee7adbff066d3acaafc2020-11-24T21:10:39ZengEmbrapa Informação TecnológicaPesquisa Agropecuária Brasileira1678-39212012-05-0147565466410.1590/S0100-204X2012000500004S0100-204X2012000500004Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of AmazoniaMariana Gomes Germano0Fabiana de Souza Cannavan1Lucas William Mendes2Amanda Barbosa Lima3Wenceslau Geraldes Teixeira4Vivian Helena Pellizari5Siu Mui Tsai6EmbrapaUniversidade de São PauloUniversidade de São PauloUniversidade de São PauloEmbrapaUniversidade de São PauloUniversidade de São PauloThe objective of this work was to evaluate the catabolic gene diversity for the bacterial degradation of aromatic hydrocarbons in anthropogenic dark earth of Amazonia (ADE) and their biochar (BC). Functional diversity analyses in ADE soils can provide information on how adaptive microorganisms may influence the fertility of soils and what is their involvement in biogeochemical cycles. For this, clone libraries containing the gene encoding for the alpha subunit of aromatic ring-hydroxylating dioxygenases (α-ARHD bacterial gene) were constructed, totaling 800 clones. These libraries were prepared from samples of an ADE soil under two different land uses, located at the Caldeirão Experimental Station - secondary forest (SF) and agriculture (AG) -, and the biochar (SF_BC and AG_BC, respectively). Heterogeneity estimates indicated greater diversity in BC libraries; and Venn diagrams showed more unique operational protein clusters (OPC) in the SF_BC library than the ADE soil, which indicates that specific metabolic processes may occur in biochar. Phylogenetic analysis showed unidentified dioxygenases in ADE soils. Libraries containing functional gene encoding for the alpha subunit of the aromatic ring-hydroxylating dioxygenases (ARHD) gene from biochar show higher diversity indices than those of ADE under secondary forest and agriculture.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000500004&lng=en&tlng=engenes catabólicosdiversidade genéticasistemas de uso da terraecologia microbianasolos tropicais |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mariana Gomes Germano Fabiana de Souza Cannavan Lucas William Mendes Amanda Barbosa Lima Wenceslau Geraldes Teixeira Vivian Helena Pellizari Siu Mui Tsai |
spellingShingle |
Mariana Gomes Germano Fabiana de Souza Cannavan Lucas William Mendes Amanda Barbosa Lima Wenceslau Geraldes Teixeira Vivian Helena Pellizari Siu Mui Tsai Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia Pesquisa Agropecuária Brasileira genes catabólicos diversidade genética sistemas de uso da terra ecologia microbiana solos tropicais |
author_facet |
Mariana Gomes Germano Fabiana de Souza Cannavan Lucas William Mendes Amanda Barbosa Lima Wenceslau Geraldes Teixeira Vivian Helena Pellizari Siu Mui Tsai |
author_sort |
Mariana Gomes Germano |
title |
Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia |
title_short |
Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia |
title_full |
Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia |
title_fullStr |
Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia |
title_full_unstemmed |
Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia |
title_sort |
functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of amazonia |
publisher |
Embrapa Informação Tecnológica |
series |
Pesquisa Agropecuária Brasileira |
issn |
1678-3921 |
publishDate |
2012-05-01 |
description |
The objective of this work was to evaluate the catabolic gene diversity for the bacterial degradation of aromatic hydrocarbons in anthropogenic dark earth of Amazonia (ADE) and their biochar (BC). Functional diversity analyses in ADE soils can provide information on how adaptive microorganisms may influence the fertility of soils and what is their involvement in biogeochemical cycles. For this, clone libraries containing the gene encoding for the alpha subunit of aromatic ring-hydroxylating dioxygenases (α-ARHD bacterial gene) were constructed, totaling 800 clones. These libraries were prepared from samples of an ADE soil under two different land uses, located at the Caldeirão Experimental Station - secondary forest (SF) and agriculture (AG) -, and the biochar (SF_BC and AG_BC, respectively). Heterogeneity estimates indicated greater diversity in BC libraries; and Venn diagrams showed more unique operational protein clusters (OPC) in the SF_BC library than the ADE soil, which indicates that specific metabolic processes may occur in biochar. Phylogenetic analysis showed unidentified dioxygenases in ADE soils. Libraries containing functional gene encoding for the alpha subunit of the aromatic ring-hydroxylating dioxygenases (ARHD) gene from biochar show higher diversity indices than those of ADE under secondary forest and agriculture. |
topic |
genes catabólicos diversidade genética sistemas de uso da terra ecologia microbiana solos tropicais |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000500004&lng=en&tlng=en |
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