Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)
The characterization and viability of pollen grains are useful tools to guide crosses in breeding programs. The objective of this study was to describe the morphological patterns and viability of pollen grains from five accessions of Passiflora edulis f. flavicarpa O. Deg. and five accessions of Pas...
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Sociedade Botânica do Brasil
2013-12-01
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doaj-4607b69457684097bd6474e29c7c648e2020-11-24T23:19:53ZengSociedade Botânica do BrasilActa Botânica Brasílica1677-941X2013-12-0127477978710.1590/S0102-33062013000400018S0102-33062013000400018Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)Taliane Leila Soares0Onildo Nunes de Jesus1Everton Hilo de Souza2Janay Almeida dos Santos-Serejo3Eder Jorge de Oliveira4Embrapa Mandioca e FruticulturaEmbrapa Mandioca e FruticulturaUniversidade de São PauloEmbrapa Mandioca e FruticulturaEmbrapa Mandioca e FruticulturaThe characterization and viability of pollen grains are useful tools to guide crosses in breeding programs. The objective of this study was to describe the morphological patterns and viability of pollen grains from five accessions of Passiflora edulis f. flavicarpa O. Deg. and five accessions of Passiflora setacea DC. Pollen morphology descriptions were made using light microscopy and scanning electron microscopy, whereas the viability analysis was performed by in vitro germination and histochemical analysis (Lugol's solution and 2,3,5-triphenyltetrazolium chloride). Pollen grains assessed for germination were inoculated in culture medium containing Ca(NO3).4H2O (0.03%), Mg(SO4).7H2O (0.02%), KNO3 (0.01%), H3BO3 (0.01%), sucrose (15%) and agar (0.8%). Although P. edulis and P. setacea showed the same shape and type of pollen aperture, the two differed in terms of their morphology and exine ornamentation pattern. In vitro analysis showed that one of the P. edulis f. flavicarpa accessions (designated BGP 330) presented the highest germination rate (53.98%) and longest pollen tube (2.18 mm). The histochemical analysis overestimated pollen viability when compared with the in vitro results. The results of this study contribute to the breeding of Passiflora species by increasing the understanding of their morphology and pollen grain viability.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400018&lng=en&tlng=enin vitro germinationhistochemistryscanning electron microscopyPassifloraceaepollen tube |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Taliane Leila Soares Onildo Nunes de Jesus Everton Hilo de Souza Janay Almeida dos Santos-Serejo Eder Jorge de Oliveira |
spellingShingle |
Taliane Leila Soares Onildo Nunes de Jesus Everton Hilo de Souza Janay Almeida dos Santos-Serejo Eder Jorge de Oliveira Morphology and viability of pollen grains from passion fruit species (Passiflora spp.) Acta Botânica Brasílica in vitro germination histochemistry scanning electron microscopy Passifloraceae pollen tube |
author_facet |
Taliane Leila Soares Onildo Nunes de Jesus Everton Hilo de Souza Janay Almeida dos Santos-Serejo Eder Jorge de Oliveira |
author_sort |
Taliane Leila Soares |
title |
Morphology and viability of pollen grains from passion fruit species (Passiflora spp.) |
title_short |
Morphology and viability of pollen grains from passion fruit species (Passiflora spp.) |
title_full |
Morphology and viability of pollen grains from passion fruit species (Passiflora spp.) |
title_fullStr |
Morphology and viability of pollen grains from passion fruit species (Passiflora spp.) |
title_full_unstemmed |
Morphology and viability of pollen grains from passion fruit species (Passiflora spp.) |
title_sort |
morphology and viability of pollen grains from passion fruit species (passiflora spp.) |
publisher |
Sociedade Botânica do Brasil |
series |
Acta Botânica Brasílica |
issn |
1677-941X |
publishDate |
2013-12-01 |
description |
The characterization and viability of pollen grains are useful tools to guide crosses in breeding programs. The objective of this study was to describe the morphological patterns and viability of pollen grains from five accessions of Passiflora edulis f. flavicarpa O. Deg. and five accessions of Passiflora setacea DC. Pollen morphology descriptions were made using light microscopy and scanning electron microscopy, whereas the viability analysis was performed by in vitro germination and histochemical analysis (Lugol's solution and 2,3,5-triphenyltetrazolium chloride). Pollen grains assessed for germination were inoculated in culture medium containing Ca(NO3).4H2O (0.03%), Mg(SO4).7H2O (0.02%), KNO3 (0.01%), H3BO3 (0.01%), sucrose (15%) and agar (0.8%). Although P. edulis and P. setacea showed the same shape and type of pollen aperture, the two differed in terms of their morphology and exine ornamentation pattern. In vitro analysis showed that one of the P. edulis f. flavicarpa accessions (designated BGP 330) presented the highest germination rate (53.98%) and longest pollen tube (2.18 mm). The histochemical analysis overestimated pollen viability when compared with the in vitro results. The results of this study contribute to the breeding of Passiflora species by increasing the understanding of their morphology and pollen grain viability. |
topic |
in vitro germination histochemistry scanning electron microscopy Passifloraceae pollen tube |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400018&lng=en&tlng=en |
work_keys_str_mv |
AT talianeleilasoares morphologyandviabilityofpollengrainsfrompassionfruitspeciespassifloraspp AT onildonunesdejesus morphologyandviabilityofpollengrainsfrompassionfruitspeciespassifloraspp AT evertonhilodesouza morphologyandviabilityofpollengrainsfrompassionfruitspeciespassifloraspp AT janayalmeidadossantosserejo morphologyandviabilityofpollengrainsfrompassionfruitspeciespassifloraspp AT ederjorgedeoliveira morphologyandviabilityofpollengrainsfrompassionfruitspeciespassifloraspp |
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