Study of the Active Components and Molecular Mechanism of Tripterygium wilfordii in the Treatment of Diabetic Nephropathy
We aimed to explore the active ingredients and molecular mechanism of Tripterygium wilfordii (TW) in the treatment of diabetic nephropathy (DN) through network pharmacology and molecular biology. First, the active ingredients and potential targets of TW were obtained through the Traditional Chinese...
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2021-06-01
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doaj-46137c97fc4849e492d2f30154ea43bc2021-06-07T05:08:34ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-06-01810.3389/fmolb.2021.664416664416Study of the Active Components and Molecular Mechanism of Tripterygium wilfordii in the Treatment of Diabetic NephropathyLin Wang0Zheyi Wang1Zhihua Yang2Kang Yang3Hongtao Yang4Graduate School, First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaDongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, ChinaGraduate School, First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaGraduate School, First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaGraduate School, First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaWe aimed to explore the active ingredients and molecular mechanism of Tripterygium wilfordii (TW) in the treatment of diabetic nephropathy (DN) through network pharmacology and molecular biology. First, the active ingredients and potential targets of TW were obtained through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and related literature materials, and Cytoscape 3.7.2 software was used to construct the active ingredient-target network diagram of TW. Second, the target set of DN was obtained through the disease database, and the potential targets of TW in the treatment of DN were screened through a Venn diagram. A protein interaction network diagram (PPI) was constructed with the help of the String platform and Cytoscape 3.7.2. Third, the ClueGO plug-in tool was used to enrich the GO biological process and the KEGG metabolic pathway. Finally, molecular docking experiments and cell pathway analyses were performed. As a result, a total of 52 active ingredients of TW were screened, and 141 predicted targets and 49 target genes related to DN were identified. The biological process of GO is mediated mainly through the regulation of oxygen metabolism, endothelial cell proliferation, acute inflammation, apoptotic signal transduction pathway, fibroblast proliferation, positive regulation of cyclase activity, adipocyte differentiation and other biological processes. KEGG enrichment analysis showed that the main pathways involved were AGE-RAGE, vascular endothelial growth factor, HIF-1, IL-17, relaxin signalling pathway, TNF, Fc epsilon RI, insulin resistance and other signaling pathways. It can be concluded that TW may treat DN by reducing inflammation, reducing antioxidative stress, regulating immunity, improving vascular disease, reducing insulin resistance, delaying renal fibrosis, repairing podocytes, and reducing cell apoptosis, among others, with multicomponent, multitarget and multisystem characteristics.https://www.frontiersin.org/articles/10.3389/fmolb.2021.664416/fullTripterygium wilfordiidiabetic nephropathyactive ingredientsmolecular mechanism of actionnetwork pharmacology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lin Wang Zheyi Wang Zhihua Yang Kang Yang Hongtao Yang |
spellingShingle |
Lin Wang Zheyi Wang Zhihua Yang Kang Yang Hongtao Yang Study of the Active Components and Molecular Mechanism of Tripterygium wilfordii in the Treatment of Diabetic Nephropathy Frontiers in Molecular Biosciences Tripterygium wilfordii diabetic nephropathy active ingredients molecular mechanism of action network pharmacology |
author_facet |
Lin Wang Zheyi Wang Zhihua Yang Kang Yang Hongtao Yang |
author_sort |
Lin Wang |
title |
Study of the Active Components and Molecular Mechanism of Tripterygium wilfordii in the Treatment of Diabetic Nephropathy |
title_short |
Study of the Active Components and Molecular Mechanism of Tripterygium wilfordii in the Treatment of Diabetic Nephropathy |
title_full |
Study of the Active Components and Molecular Mechanism of Tripterygium wilfordii in the Treatment of Diabetic Nephropathy |
title_fullStr |
Study of the Active Components and Molecular Mechanism of Tripterygium wilfordii in the Treatment of Diabetic Nephropathy |
title_full_unstemmed |
Study of the Active Components and Molecular Mechanism of Tripterygium wilfordii in the Treatment of Diabetic Nephropathy |
title_sort |
study of the active components and molecular mechanism of tripterygium wilfordii in the treatment of diabetic nephropathy |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Molecular Biosciences |
issn |
2296-889X |
publishDate |
2021-06-01 |
description |
We aimed to explore the active ingredients and molecular mechanism of Tripterygium wilfordii (TW) in the treatment of diabetic nephropathy (DN) through network pharmacology and molecular biology. First, the active ingredients and potential targets of TW were obtained through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and related literature materials, and Cytoscape 3.7.2 software was used to construct the active ingredient-target network diagram of TW. Second, the target set of DN was obtained through the disease database, and the potential targets of TW in the treatment of DN were screened through a Venn diagram. A protein interaction network diagram (PPI) was constructed with the help of the String platform and Cytoscape 3.7.2. Third, the ClueGO plug-in tool was used to enrich the GO biological process and the KEGG metabolic pathway. Finally, molecular docking experiments and cell pathway analyses were performed. As a result, a total of 52 active ingredients of TW were screened, and 141 predicted targets and 49 target genes related to DN were identified. The biological process of GO is mediated mainly through the regulation of oxygen metabolism, endothelial cell proliferation, acute inflammation, apoptotic signal transduction pathway, fibroblast proliferation, positive regulation of cyclase activity, adipocyte differentiation and other biological processes. KEGG enrichment analysis showed that the main pathways involved were AGE-RAGE, vascular endothelial growth factor, HIF-1, IL-17, relaxin signalling pathway, TNF, Fc epsilon RI, insulin resistance and other signaling pathways. It can be concluded that TW may treat DN by reducing inflammation, reducing antioxidative stress, regulating immunity, improving vascular disease, reducing insulin resistance, delaying renal fibrosis, repairing podocytes, and reducing cell apoptosis, among others, with multicomponent, multitarget and multisystem characteristics. |
topic |
Tripterygium wilfordii diabetic nephropathy active ingredients molecular mechanism of action network pharmacology |
url |
https://www.frontiersin.org/articles/10.3389/fmolb.2021.664416/full |
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