| Summary: | Oxidized low-density lipoprotein (oxLDL) and oxidized high-density lipoprotein (oxHDL), known as risk factors for cardiovascular disease, have been observed in plasma and atheromatous plaques. In a previous study, the content of oxidized phosphatidylcholine (oxPC) and lysophosphatidylcholine (lysoPC) species stayed constant in isolated in vivo oxLDL but increased in copper-induced oxLDL in vitro. In this study, we prepared synthetic deuterium-labeled 1-palmitoyl lysoPC and palmitoyl-glutaroyl PC (PGPC), a short chain-oxPC to elucidate the metabolic fate of oxPC and lysoPC in oxLDL in the presence of HDL. When LDL preloaded with d<sub>13</sub>-lysoPC was mixed with HDL, d<sub>13</sub>-lysoPC was recovered in both the LDL and HDL fractions equally. d<sub>13</sub>-LysoPC decreased by 50% after 4 h of incubation, while d<sub>13</sub>-PC increased in both fractions. Diacyl-PC production was abolished by an inhibitor of lecithin-cholesterol acyltransferase (LCAT). When d<sub>13</sub>-PGPC-preloaded LDL was incubated with HDL, d<sub>13</sub>-PGPC was transferred to HDL in a dose-dependent manner when both LCAT and lipoprotein-associated phospholipase A<sub>2</sub> (Lp-PLA<sub>2</sub>) were inhibited. Lp-PLA<sub>2</sub> in both HDL and LDL was responsible for the hydrolysis of d<sub>13</sub>-PGPC. These results suggest that short chain-oxPC and lysoPC can transfer between lipoproteins quickly and can be enzymatically converted from oxPC to lysoPC and from lysoPC to diacyl-PC in the presence of HDL.
|
|---|
