Cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversion
Current cellulosic biomass hydrolysis is based on the one-time use of cellulases. Cellulases immobilized on magnetic nanocarriers offer the advantages of magnetic separation and repeated use for continuous hydrolysis. Most immobilization methods focus on only one type of cellulase. Here, we report c...
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doaj-466fe79522284f20aca9d7dbab5bf4f52020-11-25T01:14:57ZengAIMS PressAIMS Bioengineering2375-14952016-07-013326427610.3934/bioeng.2016.3.264bioeng-03-00264Cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversionFernando Segato0Mark Wilkins1Rolf Prade2Yu Mao3Qing Song4Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078, USADepartment of Biosystems Engineering, Oklahoma State University, Stillwater, OK 74078, USADepartment of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078, USADepartment of Biosystems Engineering, Oklahoma State University, Stillwater, OK 74078, USADepartment of Immunology, Institute of Tuberculosis Control, Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou 510080, ChinaCurrent cellulosic biomass hydrolysis is based on the one-time use of cellulases. Cellulases immobilized on magnetic nanocarriers offer the advantages of magnetic separation and repeated use for continuous hydrolysis. Most immobilization methods focus on only one type of cellulase. Here, we report co-immobilization of two types of cellulases, β-glucosidase A (BglA) and cellobiohydrolase D (CelD), on sub-20 nm superparamagnetic nanoparticles. The nanoparticles demonstrated 100% immobilization efficiency for both BglA and CelD. The total enzyme activities of immobilized BglA and CelD were up to 67.1% and 41.5% of that of the free cellulases, respectively. The immobilized BglA and CelD each retained about 85% and 43% of the initial immobilized enzyme activities after being recycled 3 and 10 times, respectively. The effects of pH and temperature on the immobilized cellulases were also investigated. Co-immobilization of BglA and CelD on MNPs is a promising strategy to promote synergistic action of cellulases while lowering enzyme consumption.http://www.aimspress.com/Bioengineering/article/869/fulltext.htmlenzyme co-immobilizationβ-glucosidasecellobiohydrolasesuperparamagnetic nanoparticlescellulase reuse |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fernando Segato Mark Wilkins Rolf Prade Yu Mao Qing Song |
spellingShingle |
Fernando Segato Mark Wilkins Rolf Prade Yu Mao Qing Song Cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversion AIMS Bioengineering enzyme co-immobilization β-glucosidase cellobiohydrolase superparamagnetic nanoparticles cellulase reuse |
author_facet |
Fernando Segato Mark Wilkins Rolf Prade Yu Mao Qing Song |
author_sort |
Fernando Segato |
title |
Cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversion |
title_short |
Cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversion |
title_full |
Cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversion |
title_fullStr |
Cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversion |
title_full_unstemmed |
Cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversion |
title_sort |
cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversion |
publisher |
AIMS Press |
series |
AIMS Bioengineering |
issn |
2375-1495 |
publishDate |
2016-07-01 |
description |
Current cellulosic biomass hydrolysis is based on the one-time use of cellulases. Cellulases immobilized on magnetic nanocarriers offer the advantages of magnetic separation and repeated use for continuous hydrolysis. Most immobilization methods focus on only one type of cellulase. Here, we report co-immobilization of two types of cellulases, β-glucosidase A (BglA) and cellobiohydrolase D (CelD), on sub-20 nm superparamagnetic nanoparticles. The nanoparticles demonstrated 100% immobilization efficiency for both BglA and CelD. The total enzyme activities of immobilized BglA and CelD were up to 67.1% and 41.5% of that of the free cellulases, respectively. The immobilized BglA and CelD each retained about 85% and 43% of the initial immobilized enzyme activities after being recycled 3 and 10 times, respectively. The effects of pH and temperature on the immobilized cellulases were also investigated. Co-immobilization of BglA and CelD on MNPs is a promising strategy to promote synergistic action of cellulases while lowering enzyme consumption. |
topic |
enzyme co-immobilization β-glucosidase cellobiohydrolase superparamagnetic nanoparticles cellulase reuse |
url |
http://www.aimspress.com/Bioengineering/article/869/fulltext.html |
work_keys_str_mv |
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1725155359147950080 |