Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantation
Nicotinic acetylcholine receptors are an important class of excitatory receptors in the central nervous system of arthropods. In the ticks Ixodes ricinus, the functional and pharmacological properties of nicotinic receptors located in their neurons are still unknown. The objective of this study was...
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doaj-467d949aa231486faaf26ec095558c7f2020-12-17T04:48:09ZengElsevierInternational Journal for Parasitology: Drugs and Drug Resistance2211-32072020-12-0114144151Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantationAnaïs Le Mauff0Hamza Chouikh1Alison Cartereau2Claude L. Charvet3Cédric Neveu4Claude Rispe5Olivier Plantard6Emiliane Taillebois7Steeve H. Thany8LBLGC USC INRAE 1328, Université d'Orléans, 1 rue de Chartres, 45067, Orléans, FranceLBLGC USC INRAE 1328, Université d'Orléans, 1 rue de Chartres, 45067, Orléans, FranceLBLGC USC INRAE 1328, Université d'Orléans, 1 rue de Chartres, 45067, Orléans, FranceINRAE, Oniris, BIOEPAR, 44307, Nantes, FranceINRAE, Oniris, BIOEPAR, 44307, Nantes, FranceINRAE, ISP, 37380, Nouzilly, FranceINRAE, ISP, 37380, Nouzilly, FranceLBLGC USC INRAE 1328, Université d'Orléans, 1 rue de Chartres, 45067, Orléans, FranceLBLGC USC INRAE 1328, Université d'Orléans, 1 rue de Chartres, 45067, Orléans, France; Corresponding author.Nicotinic acetylcholine receptors are an important class of excitatory receptors in the central nervous system of arthropods. In the ticks Ixodes ricinus, the functional and pharmacological properties of nicotinic receptors located in their neurons are still unknown. The objective of this study was to characterize the pharmacological properties of tick nicotinic receptors using membrane microtransplantation in Xenopus laevis oocytes and two-electrodes voltage clamp method. The membranes microtransplanted were extracted from the tick synganglion. We found that oocytes microtransplanted with tick synganglion membranes expressed nicotinic acetylcholine receptor subtypes which were activated by acetylcholine (1 mM) and nicotine (1 mM). Currents induced by pressure application of acetylcholine and nicotine were diminished by 10 nM α-bungarotoxin and methyllycaconitine, suggesting that they expressed two subtypes of nicotinic receptors, α-bungarotoxin-sensitive and -insensitive, respectively. In addition, we found that nicotine receptors expressed in the synganglion membranes were poorly sensitive to the neonicotinoid insecticides clothianidin (CLT), imidacloprid (IMI), acetamiprid (ACE) and thiamethoxam (TMX), in agreement with their lack of activity as acaricides. Interestingly, current amplitudes were strongly potentialized in the presence of 1 μM PNU-120596. CLT was more active as an agonist than IMI, TMX and ACE. Finally, we demonstrated that microtransplantation of purified membrane from the tick synganglion can be a valuable tool for the development and screening of compounds targeting tick nicotinic acetylcholine receptor subtypes.http://www.sciencedirect.com/science/article/pii/S2211320720300361TickIxodes ricinusSynganglion membraneMicrotransplantationNicotinic acetylcholine receptors |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anaïs Le Mauff Hamza Chouikh Alison Cartereau Claude L. Charvet Cédric Neveu Claude Rispe Olivier Plantard Emiliane Taillebois Steeve H. Thany |
spellingShingle |
Anaïs Le Mauff Hamza Chouikh Alison Cartereau Claude L. Charvet Cédric Neveu Claude Rispe Olivier Plantard Emiliane Taillebois Steeve H. Thany Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantation International Journal for Parasitology: Drugs and Drug Resistance Tick Ixodes ricinus Synganglion membrane Microtransplantation Nicotinic acetylcholine receptors |
author_facet |
Anaïs Le Mauff Hamza Chouikh Alison Cartereau Claude L. Charvet Cédric Neveu Claude Rispe Olivier Plantard Emiliane Taillebois Steeve H. Thany |
author_sort |
Anaïs Le Mauff |
title |
Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantation |
title_short |
Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantation |
title_full |
Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantation |
title_fullStr |
Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantation |
title_full_unstemmed |
Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantation |
title_sort |
nicotinic acetylcholine receptors in the synganglion of the tick ixodes ricinus: functional characterization using membrane microtransplantation |
publisher |
Elsevier |
series |
International Journal for Parasitology: Drugs and Drug Resistance |
issn |
2211-3207 |
publishDate |
2020-12-01 |
description |
Nicotinic acetylcholine receptors are an important class of excitatory receptors in the central nervous system of arthropods. In the ticks Ixodes ricinus, the functional and pharmacological properties of nicotinic receptors located in their neurons are still unknown. The objective of this study was to characterize the pharmacological properties of tick nicotinic receptors using membrane microtransplantation in Xenopus laevis oocytes and two-electrodes voltage clamp method. The membranes microtransplanted were extracted from the tick synganglion. We found that oocytes microtransplanted with tick synganglion membranes expressed nicotinic acetylcholine receptor subtypes which were activated by acetylcholine (1 mM) and nicotine (1 mM). Currents induced by pressure application of acetylcholine and nicotine were diminished by 10 nM α-bungarotoxin and methyllycaconitine, suggesting that they expressed two subtypes of nicotinic receptors, α-bungarotoxin-sensitive and -insensitive, respectively. In addition, we found that nicotine receptors expressed in the synganglion membranes were poorly sensitive to the neonicotinoid insecticides clothianidin (CLT), imidacloprid (IMI), acetamiprid (ACE) and thiamethoxam (TMX), in agreement with their lack of activity as acaricides. Interestingly, current amplitudes were strongly potentialized in the presence of 1 μM PNU-120596. CLT was more active as an agonist than IMI, TMX and ACE. Finally, we demonstrated that microtransplantation of purified membrane from the tick synganglion can be a valuable tool for the development and screening of compounds targeting tick nicotinic acetylcholine receptor subtypes. |
topic |
Tick Ixodes ricinus Synganglion membrane Microtransplantation Nicotinic acetylcholine receptors |
url |
http://www.sciencedirect.com/science/article/pii/S2211320720300361 |
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