Evaluation of genetic diversity of Panicum turgidum Forssk from Saudi Arabia
The genetic diversity of 177 accessions of Panicum turgidum Forssk, representing ten populations collected from four geographical regions in Saudi Arabia, was analyzed using amplified fragment length polymorphism (AFLP) markers. A set of four primer-pairs with two/three selective nucleotides scored...
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doaj-46855e2470f141e58737d746cc17bffa2020-11-24T22:45:24ZengElsevierSaudi Journal of Biological Sciences1319-562X2018-01-0125112312910.1016/j.sjbs.2017.04.002Evaluation of genetic diversity of Panicum turgidum Forssk from Saudi ArabiaAbdulaziz M. Assaeed0Sulieman A. Al-Faifi1Hussein M. Migdadi2Magdy I. El-Bana3Abdulaziz A. Al Qarawi4Mohammad Altaf Khan5Department of Plant Production, King Saud University, PO Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Plant Production, King Saud University, PO Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Plant Production, King Saud University, PO Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Botany, Faculty of Science, Port Said University, EgyptDepartment of Plant Production, King Saud University, PO Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Plant Production, King Saud University, PO Box 2460, Riyadh 11451, Saudi ArabiaThe genetic diversity of 177 accessions of Panicum turgidum Forssk, representing ten populations collected from four geographical regions in Saudi Arabia, was analyzed using amplified fragment length polymorphism (AFLP) markers. A set of four primer-pairs with two/three selective nucleotides scored 836 AFLP amplified fragments (putative loci/genome landmarks), all of which were polymorphic. Populations collected from the southern region of the country showed the highest genetic diversity parameters, whereas those collected from the central regions showed the lowest values. Analysis of molecular variance (AMOVA) revealed that 78% of the genetic variability was attributable to differences within populations. Pairwise values for population differentiation and genetic structure were statistically significant for all variances. The UPGMA dendrogram, validated by principal coordinate analysis-grouped accessions, corresponded to the geographical origin of the accessions. Mantel’s test showed that there was a significant correlation between the genetic and geographical distances (r = 0.35, P < 0.04). In summary, the AFLP assay demonstrated the existence of substantial genetic variation in P. turgidum. The relationship between the genetic diversity and geographical source of P. turgidum populations of Saudi Arabia, as revealed through this comprehensive study, will enable effective resource management and restoration of new areas without compromising adaptation and genetic diversity.http://www.sciencedirect.com/science/article/pii/S1319562X1730116XPanicum turgidumAFLPHeterozygosityAMOVARestorationGenetic variation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abdulaziz M. Assaeed Sulieman A. Al-Faifi Hussein M. Migdadi Magdy I. El-Bana Abdulaziz A. Al Qarawi Mohammad Altaf Khan |
spellingShingle |
Abdulaziz M. Assaeed Sulieman A. Al-Faifi Hussein M. Migdadi Magdy I. El-Bana Abdulaziz A. Al Qarawi Mohammad Altaf Khan Evaluation of genetic diversity of Panicum turgidum Forssk from Saudi Arabia Saudi Journal of Biological Sciences Panicum turgidum AFLP Heterozygosity AMOVA Restoration Genetic variation |
author_facet |
Abdulaziz M. Assaeed Sulieman A. Al-Faifi Hussein M. Migdadi Magdy I. El-Bana Abdulaziz A. Al Qarawi Mohammad Altaf Khan |
author_sort |
Abdulaziz M. Assaeed |
title |
Evaluation of genetic diversity of Panicum turgidum Forssk from Saudi Arabia |
title_short |
Evaluation of genetic diversity of Panicum turgidum Forssk from Saudi Arabia |
title_full |
Evaluation of genetic diversity of Panicum turgidum Forssk from Saudi Arabia |
title_fullStr |
Evaluation of genetic diversity of Panicum turgidum Forssk from Saudi Arabia |
title_full_unstemmed |
Evaluation of genetic diversity of Panicum turgidum Forssk from Saudi Arabia |
title_sort |
evaluation of genetic diversity of panicum turgidum forssk from saudi arabia |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2018-01-01 |
description |
The genetic diversity of 177 accessions of Panicum turgidum Forssk, representing ten populations collected from four geographical regions in Saudi Arabia, was analyzed using amplified fragment length polymorphism (AFLP) markers. A set of four primer-pairs with two/three selective nucleotides scored 836 AFLP amplified fragments (putative loci/genome landmarks), all of which were polymorphic. Populations collected from the southern region of the country showed the highest genetic diversity parameters, whereas those collected from the central regions showed the lowest values. Analysis of molecular variance (AMOVA) revealed that 78% of the genetic variability was attributable to differences within populations. Pairwise values for population differentiation and genetic structure were statistically significant for all variances. The UPGMA dendrogram, validated by principal coordinate analysis-grouped accessions, corresponded to the geographical origin of the accessions. Mantel’s test showed that there was a significant correlation between the genetic and geographical distances (r = 0.35, P < 0.04). In summary, the AFLP assay demonstrated the existence of substantial genetic variation in P. turgidum. The relationship between the genetic diversity and geographical source of P. turgidum populations of Saudi Arabia, as revealed through this comprehensive study, will enable effective resource management and restoration of new areas without compromising adaptation and genetic diversity. |
topic |
Panicum turgidum AFLP Heterozygosity AMOVA Restoration Genetic variation |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X1730116X |
work_keys_str_mv |
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