The modular mechanism of chromocenter formation in Drosophila
A central principle underlying the ubiquity and abundance of pericentromeric satellite DNA repeats in eukaryotes has remained poorly understood. Previously we proposed that the interchromosomal clustering of satellite DNAs into nuclear structures known as chromocenters ensures encapsulation of all c...
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doaj-468732f2f08a48e2afe1ef543ce449ec2021-05-05T17:24:11ZengeLife Sciences Publications LtdeLife2050-084X2019-02-01810.7554/eLife.43938The modular mechanism of chromocenter formation in DrosophilaMadhav Jagannathan0https://orcid.org/0000-0003-3428-6812Ryan Cummings1https://orcid.org/0000-0003-0540-9174Yukiko M Yamashita2https://orcid.org/0000-0001-5541-0216Life Sciences Institute, University of Michigan, Ann Arbor, United StatesHoward Hughes Medical Institute, University of Michigan, Ann Arbor, United StatesHoward Hughes Medical Institute, University of Michigan, Ann Arbor, United States; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, United StatesA central principle underlying the ubiquity and abundance of pericentromeric satellite DNA repeats in eukaryotes has remained poorly understood. Previously we proposed that the interchromosomal clustering of satellite DNAs into nuclear structures known as chromocenters ensures encapsulation of all chromosomes into a single nucleus (Jagannathan et al., 2018). Chromocenter disruption led to micronuclei formation, resulting in cell death. Here we show that chromocenter formation is mediated by a ‘modular’ network, where associations between two sequence-specific satellite DNA-binding proteins, D1 and Prod, bound to their cognate satellite DNAs, bring the full complement of chromosomes into the chromocenter. D1 prod double mutants die during embryogenesis, exhibiting enhanced phenotypes associated with chromocenter disruption, revealing the universal importance of satellite DNAs and chromocenters. Taken together, we propose that associations between chromocenter modules, consisting of satellite DNA binding proteins and their cognate satellite DNA, package the Drosophila genome within a single nucleus.https://elifesciences.org/articles/43938chromocentersatellite DNAmicronuclei |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Madhav Jagannathan Ryan Cummings Yukiko M Yamashita |
spellingShingle |
Madhav Jagannathan Ryan Cummings Yukiko M Yamashita The modular mechanism of chromocenter formation in Drosophila eLife chromocenter satellite DNA micronuclei |
author_facet |
Madhav Jagannathan Ryan Cummings Yukiko M Yamashita |
author_sort |
Madhav Jagannathan |
title |
The modular mechanism of chromocenter formation in Drosophila |
title_short |
The modular mechanism of chromocenter formation in Drosophila |
title_full |
The modular mechanism of chromocenter formation in Drosophila |
title_fullStr |
The modular mechanism of chromocenter formation in Drosophila |
title_full_unstemmed |
The modular mechanism of chromocenter formation in Drosophila |
title_sort |
modular mechanism of chromocenter formation in drosophila |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2019-02-01 |
description |
A central principle underlying the ubiquity and abundance of pericentromeric satellite DNA repeats in eukaryotes has remained poorly understood. Previously we proposed that the interchromosomal clustering of satellite DNAs into nuclear structures known as chromocenters ensures encapsulation of all chromosomes into a single nucleus (Jagannathan et al., 2018). Chromocenter disruption led to micronuclei formation, resulting in cell death. Here we show that chromocenter formation is mediated by a ‘modular’ network, where associations between two sequence-specific satellite DNA-binding proteins, D1 and Prod, bound to their cognate satellite DNAs, bring the full complement of chromosomes into the chromocenter. D1 prod double mutants die during embryogenesis, exhibiting enhanced phenotypes associated with chromocenter disruption, revealing the universal importance of satellite DNAs and chromocenters. Taken together, we propose that associations between chromocenter modules, consisting of satellite DNA binding proteins and their cognate satellite DNA, package the Drosophila genome within a single nucleus. |
topic |
chromocenter satellite DNA micronuclei |
url |
https://elifesciences.org/articles/43938 |
work_keys_str_mv |
AT madhavjagannathan themodularmechanismofchromocenterformationindrosophila AT ryancummings themodularmechanismofchromocenterformationindrosophila AT yukikomyamashita themodularmechanismofchromocenterformationindrosophila AT madhavjagannathan modularmechanismofchromocenterformationindrosophila AT ryancummings modularmechanismofchromocenterformationindrosophila AT yukikomyamashita modularmechanismofchromocenterformationindrosophila |
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