Colistin susceptibility among multidrug resistant Gram-negative bacilli isolated from Tertiary hospital in Egypt
Colistin is considered as the last resort for treatment of bacterial infections caused by carbapenem-resistant Gram-negative bacilli (GNB). Colistin resistance can increase due to the spread of plasmid-borne mcr-1 gene. This study aimed to determine colistin susceptibility and to detect the presen...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Ain Shams University
2020-10-01
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Series: | Novel Research in Microbiology Journal |
Subjects: | |
Online Access: | https://nrmj.journals.ekb.eg/article_118447_9f6e24fc5137c5c18bfb8924fa7d6e16.pdf |
Summary: | Colistin is considered as the last resort for treatment of bacterial infections caused by carbapenem-resistant
Gram-negative bacilli (GNB). Colistin resistance can increase due to the spread of plasmid-borne mcr-1 gene.
This study aimed to determine colistin susceptibility and to detect the presence of mcr-1 gene in the clinical
isolates of GNB recovered from different clinical samples collected from Ain Shams University Hospital, Cairo,
Egypt. About thirty-five GNB isolates were recovered from the different clinical samples that were collected
during the period from February-April, 2019. These isolates were subjected to antibiotic susceptibility testing
using disc diffusion assay, and Colistin susceptibility through the E-test. In addition, conventional Polymerase
chain reaction (PCR) was carried out for detection of mcr-1 gene among the colistin GNB resistant isolates. Most
of the GNB isolates (60 %) were recovered from blood samples. Klebsiella pneumoniae (K. pneumoniae) was the
most common isolated bacterium; that was represented by 24 isolates (68%). Out of the 35 GNB, only five
isolates (14.3 %) were resistant to colistin by E-test, with MIC >256 𝜇g/ ml. The mcr-1 gene was detected only in
one Pseudomonas aeruginosa (P. aeruginosa) isolate. This study concluded the low frequency of mcr-1 gene
among the current GNB isolates. However, a large scale study is recommended to detect colistin resistance among
GNB. |
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ISSN: | 2537-0286 2537-0294 |