The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle

• Main Authors: Faye M Nixon, Cristina Gutiérrez-Caballero, Fiona E Hood, Daniel G Booth, Ian A Prior, Stephen J Royle
• Format: Article
• Language: English
• Published: eLife Sciences Publications Ltd 2015-06-01
• Series: eLife
• Subjects: electron microscopy; microtubule; mitosis; cancer
• Online Access: https://elifesciences.org/articles/07635
• ISSN: 2050-084X

Kinetochore fibers (K-fibers) of the mitotic spindle are force-generating units that power chromosome movement during mitosis. K-fibers are composed of many microtubules that are held together throughout their length. Here, we show, using 3D electron microscopy, that K-fiber microtubules (MTs) are connected by a network of MT connectors. We term this network ‘the mesh’. The K-fiber mesh is made of linked multipolar connectors. Each connector has up to four struts, so that a single connector can link up to four MTs. Molecular manipulation of the mesh by overexpression of TACC3 causes disorganization of the K-fiber MTs. Optimal stabilization of K-fibers by the mesh is required for normal progression through mitosis. We propose that the mesh stabilizes K-fibers by pulling MTs together and thereby maintaining the integrity of the fiber. Our work thus identifies the K-fiber meshwork of linked multipolar connectors as a key integrator and determinant of K-fiber structure and function.