Ceasing down Pseudomonas aeruginosa Invasiveness in A Mouse Burn Wound Sepsis Model by Recombinant OprF

• Main Authors: Zohreh Rasooli, Parviz Owlia, Shakiba Darvish-Alipour-Astaneh
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2015-10-01
• Series: Journal of Medical Bacteriology
• Subjects: Pseudomonas aeruginosa; Opr; Infection Burn; Vaccine
• Online Access: https://jmb.tums.ac.ir/index.php/jmb/article/view/17
• ISSN: 2251-8649; 2322-2581

Background: Bacterial infections in burn and wound patients are common and difficult to control. The aim of the current study was to evaluate the ability of full length OprF to elicit the production of protective IgG in mice burn wound sepsis model against P. aeruginosa infection. Methods: OprF protein was expressed and purified by Ni-NTA. The purified protein as used to immunize BALB/c mice. The antibody raised against OprF was confirmed by ELISA and evaluated by immunoblot analysis. After burn and bacterial challenge, mortality rate was monitored in the control and immunized mice groups. Bacterial quantity in skin, blood, spleen and liver was evaluated to study spread or inhibition of the infection. Results: Immunization of mice with OprF brought about a significant rise in anti-OprF sera titer. Protection was imparted in the immunized group resulting in 100% survival against 1000 fold LD50 challenge with P. aeruginosa. The antiserum against OprF was able to significantly inhibit the systemic spread of P. aeruginosa infection from the infection site to internal organs. Conclusions: The results suggest that anti-P. aeruginosa OprF antibodies elicited in burn wound sepsis model by active immunization are protective against infection with P. aeruginosa, and provide a rational for further development of the vaccine for prevention against P. aeruginosa infection in burn patients.