Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.)

Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.)

In this paper the complete cDNA sequence of a newly identified triticale phytocystatin, TrcC-7, was analyzed. Because TrcC-7 transcripts were present in seeds, we hypothesized that it may regulate storage protein accumulation and degradation. Therefore, changes in mRNA and protein levels during the...

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Main Authors: Joanna Simińska, Wiesław Bielawski
Format: Article
Language:English
Published: Polish Botanical Society 2015-03-01
Series:Acta Societatis Botanicorum Poloniae
Subjects:
phytocystatin
cysteine proteinase inhibitor
seed development
germination
Online Access:https://pbsociety.org.pl/journals/index.php/asbp/article/view/4234
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spelling doaj-46de169aa0ba4758a1cf756b0809c9e62020-11-25T03:11:10ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802015-03-0184113914210.5586/asbp.2015.0113991Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.)Joanna Simińska0Wiesław Bielawski1Department of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences – SGGW, Nowoursynowska 159, 02-776 WarsawDepartment of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences – SGGW, Nowoursynowska 159, 02-776 WarsawIn this paper the complete cDNA sequence of a newly identified triticale phytocystatin, TrcC-7, was analyzed. Because TrcC-7 transcripts were present in seeds, we hypothesized that it may regulate storage protein accumulation and degradation. Therefore, changes in mRNA and protein levels during the entire period of seed development and germination were examined. Expression of TrcC-7 increased during development and decreased at the end of maturation and subsequently increased during seed germination. Based on these results, TrcC-7 likely regulates cysteine proteinase activity during the accumulation and mobilization of storage proteins.https://pbsociety.org.pl/journals/index.php/asbp/article/view/4234phytocystatincysteine proteinase inhibitorseed developmentgermination
collection DOAJ
language English
format Article
sources DOAJ
author Joanna Simińska
Wiesław Bielawski
spellingShingle Joanna Simińska
Wiesław Bielawski
Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.)
Acta Societatis Botanicorum Poloniae
phytocystatin
cysteine proteinase inhibitor
seed development
germination
author_facet Joanna Simińska
Wiesław Bielawski
author_sort Joanna Simińska
title Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.)
title_short Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.)
title_full Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.)
title_fullStr Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.)
title_full_unstemmed Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.)
title_sort identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×triticosecale wittm.)
publisher Polish Botanical Society
series Acta Societatis Botanicorum Poloniae
issn 2083-9480
publishDate 2015-03-01
description In this paper the complete cDNA sequence of a newly identified triticale phytocystatin, TrcC-7, was analyzed. Because TrcC-7 transcripts were present in seeds, we hypothesized that it may regulate storage protein accumulation and degradation. Therefore, changes in mRNA and protein levels during the entire period of seed development and germination were examined. Expression of TrcC-7 increased during development and decreased at the end of maturation and subsequently increased during seed germination. Based on these results, TrcC-7 likely regulates cysteine proteinase activity during the accumulation and mobilization of storage proteins.
topic phytocystatin
cysteine proteinase inhibitor
seed development
germination
url https://pbsociety.org.pl/journals/index.php/asbp/article/view/4234
work_keys_str_mv AT joannasiminska identificationandexpressionanalysisofanovelphytocystatinindevelopingandgerminatingseedsoftriticaletriticosecalewittm
AT wiesławbielawski identificationandexpressionanalysisofanovelphytocystatinindevelopingandgerminatingseedsoftriticaletriticosecalewittm
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