Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.)
In this paper the complete cDNA sequence of a newly identified triticale phytocystatin, TrcC-7, was analyzed. Because TrcC-7 transcripts were present in seeds, we hypothesized that it may regulate storage protein accumulation and degradation. Therefore, changes in mRNA and protein levels during the...
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Polish Botanical Society
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doaj-46de169aa0ba4758a1cf756b0809c9e62020-11-25T03:11:10ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802015-03-0184113914210.5586/asbp.2015.0113991Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.)Joanna Simińska0Wiesław Bielawski1Department of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences – SGGW, Nowoursynowska 159, 02-776 WarsawDepartment of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences – SGGW, Nowoursynowska 159, 02-776 WarsawIn this paper the complete cDNA sequence of a newly identified triticale phytocystatin, TrcC-7, was analyzed. Because TrcC-7 transcripts were present in seeds, we hypothesized that it may regulate storage protein accumulation and degradation. Therefore, changes in mRNA and protein levels during the entire period of seed development and germination were examined. Expression of TrcC-7 increased during development and decreased at the end of maturation and subsequently increased during seed germination. Based on these results, TrcC-7 likely regulates cysteine proteinase activity during the accumulation and mobilization of storage proteins.https://pbsociety.org.pl/journals/index.php/asbp/article/view/4234phytocystatincysteine proteinase inhibitorseed developmentgermination |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Joanna Simińska Wiesław Bielawski |
spellingShingle |
Joanna Simińska Wiesław Bielawski Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.) Acta Societatis Botanicorum Poloniae phytocystatin cysteine proteinase inhibitor seed development germination |
author_facet |
Joanna Simińska Wiesław Bielawski |
author_sort |
Joanna Simińska |
title |
Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.) |
title_short |
Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.) |
title_full |
Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.) |
title_fullStr |
Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.) |
title_full_unstemmed |
Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×Triticosecale Wittm.) |
title_sort |
identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (×triticosecale wittm.) |
publisher |
Polish Botanical Society |
series |
Acta Societatis Botanicorum Poloniae |
issn |
2083-9480 |
publishDate |
2015-03-01 |
description |
In this paper the complete cDNA sequence of a newly identified triticale phytocystatin, TrcC-7, was analyzed. Because TrcC-7 transcripts were present in seeds, we hypothesized that it may regulate storage protein accumulation and degradation. Therefore, changes in mRNA and protein levels during the entire period of seed development and germination were examined. Expression of TrcC-7 increased during development and decreased at the end of maturation and subsequently increased during seed germination. Based on these results, TrcC-7 likely regulates cysteine proteinase activity during the accumulation and mobilization of storage proteins. |
topic |
phytocystatin cysteine proteinase inhibitor seed development germination |
url |
https://pbsociety.org.pl/journals/index.php/asbp/article/view/4234 |
work_keys_str_mv |
AT joannasiminska identificationandexpressionanalysisofanovelphytocystatinindevelopingandgerminatingseedsoftriticaletriticosecalewittm AT wiesławbielawski identificationandexpressionanalysisofanovelphytocystatinindevelopingandgerminatingseedsoftriticaletriticosecalewittm |
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1724655690824286208 |