Adoptive transfer of type 1 regulatory T cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model mice
Type 1 regulatory T (Tr1) cells are CD4+ T cells that produce a large amount of IL-10, an anti-inflammatory cytokine. However, it has not been fully elucidated whether Tr1 cells suppress allergic asthma. In this study, the effects of adoptive transfer of in vitro-induced Tr1 cells on allergic asthma...
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doaj-46df5781e0b94efc80ee5ef7aca125c12020-11-25T01:47:59ZengElsevierJournal of Pharmacological Sciences1347-86132019-12-011414139145Adoptive transfer of type 1 regulatory T cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model miceMasaya Matsuda0Kana Doi1Tatsuya Tsutsumi2Miki Inaba3Junpei Hamaguchi4Tetsuya Terada5Ryo Kawata6Kazuyuki Kitatani7Takeshi Nabe8Laboratory of Immunopharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Immunopharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Immunopharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Immunopharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Immunopharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanDepartment of Otolaryngology, Head and Neck Surgery, Osaka Medical College, Osaka, JapanDepartment of Otolaryngology, Head and Neck Surgery, Osaka Medical College, Osaka, JapanLaboratory of Immunopharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Immunopharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan; Corresponding author. Laboratory of Immunopharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, 45-1 Nagaotouge-cho, Hirakata, Osaka 573-0101, Japan. Fax: +81 72 807 6074.Type 1 regulatory T (Tr1) cells are CD4+ T cells that produce a large amount of IL-10, an anti-inflammatory cytokine. However, it has not been fully elucidated whether Tr1 cells suppress allergic asthma. In this study, the effects of adoptive transfer of in vitro-induced Tr1 cells on allergic asthma were evaluated. Splenocytes from ovalbumin (OVA)-sensitized BALB/c mice were cultured with OVA, IL-21, IL-27, and TGF-β. After culture, IL-10-producing CD4+ T cells were isolated by Dynabeads mouse CD4 and IL-10 secretion assay, and analyzed by flow cytometry. Purified Tr1 cells (IL-10+ CD4+ T cells) were intravenously injected into OVA-sensitized BALB/c mice. The recipient mice were intratracheally challenged with OVA. Airway hyperresponsiveness to methacholine was assessed by the forced oscillation technique, followed by bronchoalveolar lavage (BAL). Almost all of the induced IL-10-producing CD4+ T cells were negative for interferon-γ, IL-4, IL-17A, and forkhead box P3, suggesting that the cells were Tr1 cells. The adoptive transfer of Tr1 cells significantly suppressed the development of airway hyperresponsiveness, and increases in IL-5, eosinophils, and neutrophils in BAL fluid. In conclusion, we demonstrated that Tr1 cells suppressed allergic asthma in mice. Keywords: Asthma, Airway hyperresponsiveness, IL-10, Immunotherapy, T cellhttp://www.sciencedirect.com/science/article/pii/S1347861319357226 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Masaya Matsuda Kana Doi Tatsuya Tsutsumi Miki Inaba Junpei Hamaguchi Tetsuya Terada Ryo Kawata Kazuyuki Kitatani Takeshi Nabe |
spellingShingle |
Masaya Matsuda Kana Doi Tatsuya Tsutsumi Miki Inaba Junpei Hamaguchi Tetsuya Terada Ryo Kawata Kazuyuki Kitatani Takeshi Nabe Adoptive transfer of type 1 regulatory T cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model mice Journal of Pharmacological Sciences |
author_facet |
Masaya Matsuda Kana Doi Tatsuya Tsutsumi Miki Inaba Junpei Hamaguchi Tetsuya Terada Ryo Kawata Kazuyuki Kitatani Takeshi Nabe |
author_sort |
Masaya Matsuda |
title |
Adoptive transfer of type 1 regulatory T cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model mice |
title_short |
Adoptive transfer of type 1 regulatory T cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model mice |
title_full |
Adoptive transfer of type 1 regulatory T cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model mice |
title_fullStr |
Adoptive transfer of type 1 regulatory T cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model mice |
title_full_unstemmed |
Adoptive transfer of type 1 regulatory T cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model mice |
title_sort |
adoptive transfer of type 1 regulatory t cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model mice |
publisher |
Elsevier |
series |
Journal of Pharmacological Sciences |
issn |
1347-8613 |
publishDate |
2019-12-01 |
description |
Type 1 regulatory T (Tr1) cells are CD4+ T cells that produce a large amount of IL-10, an anti-inflammatory cytokine. However, it has not been fully elucidated whether Tr1 cells suppress allergic asthma. In this study, the effects of adoptive transfer of in vitro-induced Tr1 cells on allergic asthma were evaluated. Splenocytes from ovalbumin (OVA)-sensitized BALB/c mice were cultured with OVA, IL-21, IL-27, and TGF-β. After culture, IL-10-producing CD4+ T cells were isolated by Dynabeads mouse CD4 and IL-10 secretion assay, and analyzed by flow cytometry. Purified Tr1 cells (IL-10+ CD4+ T cells) were intravenously injected into OVA-sensitized BALB/c mice. The recipient mice were intratracheally challenged with OVA. Airway hyperresponsiveness to methacholine was assessed by the forced oscillation technique, followed by bronchoalveolar lavage (BAL). Almost all of the induced IL-10-producing CD4+ T cells were negative for interferon-γ, IL-4, IL-17A, and forkhead box P3, suggesting that the cells were Tr1 cells. The adoptive transfer of Tr1 cells significantly suppressed the development of airway hyperresponsiveness, and increases in IL-5, eosinophils, and neutrophils in BAL fluid. In conclusion, we demonstrated that Tr1 cells suppressed allergic asthma in mice. Keywords: Asthma, Airway hyperresponsiveness, IL-10, Immunotherapy, T cell |
url |
http://www.sciencedirect.com/science/article/pii/S1347861319357226 |
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