Haematological changes in Nile tilapia experimentally infected with Enterococcus sp.
This study evaluated the haematological changes in Nile tilapia experimentally infected with 1 x 10³ and 1 x 10(6) colony-forming units (CFU)/mL of Enterococcus sp. in the swim bladder. The experiment consisted of four treatments in triplicates: non-injected fish (NI); fish injected with 1 mL of ste...
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doaj-46f37dc2522c43da85561453ee36edb52020-11-24T22:36:00ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-437568365766110.1590/S1519-69842008000300025S1519-69842008000300025Haematological changes in Nile tilapia experimentally infected with Enterococcus sp.ML. Martins0JLP. Mouriño1GV. Amaral2FN. Vieira3G. Dotta4AMB. Jatobá5FS. Pedrotti6GT. Jerônimo7CC. Buglione-Neto8G. Pereira-Jr.9Universidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaThis study evaluated the haematological changes in Nile tilapia experimentally infected with 1 x 10³ and 1 x 10(6) colony-forming units (CFU)/mL of Enterococcus sp. in the swim bladder. The experiment consisted of four treatments in triplicates: non-injected fish (NI); fish injected with 1 mL of sterile saline solution 0.65% (SAL); fish injected with 1 x 10³ and 1 x 10(6) CFU/mL of Enterococcus diluted in 1 mL sterile saline. Twenty-four hours after injection, the fish were anesthetized and the blood collected. The haematological tests included red blood cell (RBC) and white blood cell (WBC) counts, hematocrit, number of total thrombocytes, and differential counting of WBC. Fish injected with 1 x 10(6) CFU/mL of Enterococcus showed a higher number of thrombocytes than the other treatments. White blood cell and lymphocyte numbers increased significantly in fish injected with 1 x 10(6) CFU/mL of Enterococcus when compared to non-injected control. There was significant increase in the number of neutrophils in saline injected fish and reduced number of monocytes after injections with 1 x 10(6) CFU/mL of Enterococcus. Hematocrit increased in fish injected with 1 x 10³ and 1 x 10(6) CFU/mL of Enterococcus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842008000300025&lng=en&tlng=entilapiaEnterococcusinfectionhaematology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
ML. Martins JLP. Mouriño GV. Amaral FN. Vieira G. Dotta AMB. Jatobá FS. Pedrotti GT. Jerônimo CC. Buglione-Neto G. Pereira-Jr. |
spellingShingle |
ML. Martins JLP. Mouriño GV. Amaral FN. Vieira G. Dotta AMB. Jatobá FS. Pedrotti GT. Jerônimo CC. Buglione-Neto G. Pereira-Jr. Haematological changes in Nile tilapia experimentally infected with Enterococcus sp. Brazilian Journal of Biology tilapia Enterococcus infection haematology |
author_facet |
ML. Martins JLP. Mouriño GV. Amaral FN. Vieira G. Dotta AMB. Jatobá FS. Pedrotti GT. Jerônimo CC. Buglione-Neto G. Pereira-Jr. |
author_sort |
ML. Martins |
title |
Haematological changes in Nile tilapia experimentally infected with Enterococcus sp. |
title_short |
Haematological changes in Nile tilapia experimentally infected with Enterococcus sp. |
title_full |
Haematological changes in Nile tilapia experimentally infected with Enterococcus sp. |
title_fullStr |
Haematological changes in Nile tilapia experimentally infected with Enterococcus sp. |
title_full_unstemmed |
Haematological changes in Nile tilapia experimentally infected with Enterococcus sp. |
title_sort |
haematological changes in nile tilapia experimentally infected with enterococcus sp. |
publisher |
Instituto Internacional de Ecologia |
series |
Brazilian Journal of Biology |
issn |
1678-4375 |
description |
This study evaluated the haematological changes in Nile tilapia experimentally infected with 1 x 10³ and 1 x 10(6) colony-forming units (CFU)/mL of Enterococcus sp. in the swim bladder. The experiment consisted of four treatments in triplicates: non-injected fish (NI); fish injected with 1 mL of sterile saline solution 0.65% (SAL); fish injected with 1 x 10³ and 1 x 10(6) CFU/mL of Enterococcus diluted in 1 mL sterile saline. Twenty-four hours after injection, the fish were anesthetized and the blood collected. The haematological tests included red blood cell (RBC) and white blood cell (WBC) counts, hematocrit, number of total thrombocytes, and differential counting of WBC. Fish injected with 1 x 10(6) CFU/mL of Enterococcus showed a higher number of thrombocytes than the other treatments. White blood cell and lymphocyte numbers increased significantly in fish injected with 1 x 10(6) CFU/mL of Enterococcus when compared to non-injected control. There was significant increase in the number of neutrophils in saline injected fish and reduced number of monocytes after injections with 1 x 10(6) CFU/mL of Enterococcus. Hematocrit increased in fish injected with 1 x 10³ and 1 x 10(6) CFU/mL of Enterococcus. |
topic |
tilapia Enterococcus infection haematology |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842008000300025&lng=en&tlng=en |
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