Antifungal Susceptibility Patterns of Candida Species Recovered from Endotracheal Tube in an Intensive Care Unit
Aims. Biofilms formed by Candida species which associated with drastically enhanced resistance against most antimicrobial agents. The aim of this study was to identify and determine the antifungal susceptibility pattern of Candida species isolated from endotracheal tubes from ICU patients. Methods....
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doaj-46f6fa89d0f045c69fe6fcd7797439452020-11-25T00:37:38ZengHindawi LimitedAdvances in Medicine2356-67522314-758X2016-01-01201610.1155/2016/92420319242031Antifungal Susceptibility Patterns of Candida Species Recovered from Endotracheal Tube in an Intensive Care UnitElham Baghdadi0Sadegh Khodavaisy1Sassan Rezaie2Sara Abolghasem3Neda Kiasat4Zahra Salehi5Somayeh Sharifynia6Farzad Aala7Department of Microbiology, Faculty of Science, Islamic Azad University, Varamin-Pishva Branch, Tehran, IranDepartment of Medical Parasitology and Mycology, Kurdistan University of Medical Sciences, P.O. Box 14155-6446, Sanandaj, IranDivision of Molecular Biology, Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Microbiology, Faculty of Science, Islamic Azad University, North Tehran Branch, Tehran, IranDepartment of Medical Mycology, Jondishapour University of Medical Sciences, Ahvaz, IranDepartment of Medical Mycology, Tarbiat Modares University, Tehran, IranClinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Medical Parasitology and Mycology, Kurdistan University of Medical Sciences, P.O. Box 14155-6446, Sanandaj, IranAims. Biofilms formed by Candida species which associated with drastically enhanced resistance against most antimicrobial agents. The aim of this study was to identify and determine the antifungal susceptibility pattern of Candida species isolated from endotracheal tubes from ICU patients. Methods. One hundred forty ICU patients with tracheal tubes who were intubated and mechanically ventilated were surveyed for endotracheal tube biofilms. Samples were processed for quantitative microbial culture. Yeast isolates were identified to the species level based on morphological characteristics and their identity was confirmed by PCR-RFLP. Antifungal susceptibility testing was determined according to CLSI document (M27-A3). Results. Ninety-five strains of Candida were obtained from endotracheal tubes of which C. albicans (n=34; 35.7%) was the most frequently isolated species followed by other species which included C. glabrata (n=24; 25.2%), C. parapsilosis (n=16; 16.8%), C. tropicalis (n=12; 12.6%), and C. krusei (n=9; 9.4%). The resulting MIC90 for all Candida species were in increasing order as follows: caspofungin (0.5 μg/mL); amphotericin B (2 μg/mL); voriconazole (8.8 μg/mL); itraconazole (16 μg/mL); and fluconazole (64 μg/mL). Conclusion. Candida species recovered from endotracheal tube are the most susceptible to caspofungin.http://dx.doi.org/10.1155/2016/9242031 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elham Baghdadi Sadegh Khodavaisy Sassan Rezaie Sara Abolghasem Neda Kiasat Zahra Salehi Somayeh Sharifynia Farzad Aala |
spellingShingle |
Elham Baghdadi Sadegh Khodavaisy Sassan Rezaie Sara Abolghasem Neda Kiasat Zahra Salehi Somayeh Sharifynia Farzad Aala Antifungal Susceptibility Patterns of Candida Species Recovered from Endotracheal Tube in an Intensive Care Unit Advances in Medicine |
author_facet |
Elham Baghdadi Sadegh Khodavaisy Sassan Rezaie Sara Abolghasem Neda Kiasat Zahra Salehi Somayeh Sharifynia Farzad Aala |
author_sort |
Elham Baghdadi |
title |
Antifungal Susceptibility Patterns of Candida Species Recovered from Endotracheal Tube in an Intensive Care Unit |
title_short |
Antifungal Susceptibility Patterns of Candida Species Recovered from Endotracheal Tube in an Intensive Care Unit |
title_full |
Antifungal Susceptibility Patterns of Candida Species Recovered from Endotracheal Tube in an Intensive Care Unit |
title_fullStr |
Antifungal Susceptibility Patterns of Candida Species Recovered from Endotracheal Tube in an Intensive Care Unit |
title_full_unstemmed |
Antifungal Susceptibility Patterns of Candida Species Recovered from Endotracheal Tube in an Intensive Care Unit |
title_sort |
antifungal susceptibility patterns of candida species recovered from endotracheal tube in an intensive care unit |
publisher |
Hindawi Limited |
series |
Advances in Medicine |
issn |
2356-6752 2314-758X |
publishDate |
2016-01-01 |
description |
Aims. Biofilms formed by Candida species which associated with drastically enhanced resistance against most antimicrobial agents. The aim of this study was to identify and determine the antifungal susceptibility pattern of Candida species isolated from endotracheal tubes from ICU patients. Methods. One hundred forty ICU patients with tracheal tubes who were intubated and mechanically ventilated were surveyed for endotracheal tube biofilms. Samples were processed for quantitative microbial culture. Yeast isolates were identified to the species level based on morphological characteristics and their identity was confirmed by PCR-RFLP. Antifungal susceptibility testing was determined according to CLSI document (M27-A3). Results. Ninety-five strains of Candida were obtained from endotracheal tubes of which C. albicans (n=34; 35.7%) was the most frequently isolated species followed by other species which included C. glabrata (n=24; 25.2%), C. parapsilosis (n=16; 16.8%), C. tropicalis (n=12; 12.6%), and C. krusei (n=9; 9.4%). The resulting MIC90 for all Candida species were in increasing order as follows: caspofungin (0.5 μg/mL); amphotericin B (2 μg/mL); voriconazole (8.8 μg/mL); itraconazole (16 μg/mL); and fluconazole (64 μg/mL). Conclusion. Candida species recovered from endotracheal tube are the most susceptible to caspofungin. |
url |
http://dx.doi.org/10.1155/2016/9242031 |
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