Characterization of the Esophageal Microbiota and Prediction of the Metabolic Pathways Involved in Esophageal Cancer
Esophageal microbiota plays important roles in esophageal cancer. Esophagectomy, as the most important therapeutic way, contributes to changes of esophageal microbiome. However, there are few studies examining the esophageal microbiome and the metabolic changes before and after esophagectomy. The pr...
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doaj-4706f6bc248a451eae3b67d3a65f55742020-11-25T02:58:36ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-06-011010.3389/fcimb.2020.00268544511Characterization of the Esophageal Microbiota and Prediction of the Metabolic Pathways Involved in Esophageal CancerDonghang Li0Ruyuan He1Guoqiang Hou2Wei Ming3Tao Fan4Lei Chen5Lin Zhang6Wenyang Jiang7Wei Wang8Zilong Lu9Haojie Feng10Qing Geng11Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Thoracic Surgery, Yangxin County People's Hospital, Yangxin, ChinaDepartment of Thoracic Surgery, Yangxin County People's Hospital, Yangxin, ChinaDepartment of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaEsophageal microbiota plays important roles in esophageal cancer. Esophagectomy, as the most important therapeutic way, contributes to changes of esophageal microbiome. However, there are few studies examining the esophageal microbiome and the metabolic changes before and after esophagectomy. The present study characterized the esophageal microbiome of 17 patients with esophageal squamous cell carcinoma (ESCC), 11 patients with esophagogastric junction (EGJ) cancer, 15 patients at 9–12 months after radical esophagectomy and 16 healthy controls (HC). 16S ribosomal RNA gene sequencing was used to evaluate the microbiome and predict the metabolic pathways. Our results showed that the microbial diversity was significantly lower in ESCC, EGJ and post-ESCC groups than that in the HC group. The abundance of Fusobacteria was higher (7.01 vs. 1.12%, P = 0.039) and the abundance of Actinobacteria (1.61 vs. 4.04%) was lower in the ESCC group than that in the HC group. We found significant differences in the abundance of Bacteroidetes (20.45 vs. 9.86%, P = 0.026), Fusobacteria (7.01 vs. 1.66%, P = 0.030) between ESCC and post-ESCC groups. The results of microbial composition analysis and PICRUSt demonstrated significant differences between ESCC and HC groups. The β diversity and PICRUSt suggested that the microbial composition and metabolic pathways were similar to HC group after esophagectomy. The monitoring of the esophagus microbiota may be an essential method to predict the recurrence of tumor.https://www.frontiersin.org/article/10.3389/fcimb.2020.00268/fullmicrobiotaesophageal squamous cell carcinomaesophagectomy16S ribosomal RNA gene sequencingfusobacteria |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Donghang Li Ruyuan He Guoqiang Hou Wei Ming Tao Fan Lei Chen Lin Zhang Wenyang Jiang Wei Wang Zilong Lu Haojie Feng Qing Geng |
spellingShingle |
Donghang Li Ruyuan He Guoqiang Hou Wei Ming Tao Fan Lei Chen Lin Zhang Wenyang Jiang Wei Wang Zilong Lu Haojie Feng Qing Geng Characterization of the Esophageal Microbiota and Prediction of the Metabolic Pathways Involved in Esophageal Cancer Frontiers in Cellular and Infection Microbiology microbiota esophageal squamous cell carcinoma esophagectomy 16S ribosomal RNA gene sequencing fusobacteria |
author_facet |
Donghang Li Ruyuan He Guoqiang Hou Wei Ming Tao Fan Lei Chen Lin Zhang Wenyang Jiang Wei Wang Zilong Lu Haojie Feng Qing Geng |
author_sort |
Donghang Li |
title |
Characterization of the Esophageal Microbiota and Prediction of the Metabolic Pathways Involved in Esophageal Cancer |
title_short |
Characterization of the Esophageal Microbiota and Prediction of the Metabolic Pathways Involved in Esophageal Cancer |
title_full |
Characterization of the Esophageal Microbiota and Prediction of the Metabolic Pathways Involved in Esophageal Cancer |
title_fullStr |
Characterization of the Esophageal Microbiota and Prediction of the Metabolic Pathways Involved in Esophageal Cancer |
title_full_unstemmed |
Characterization of the Esophageal Microbiota and Prediction of the Metabolic Pathways Involved in Esophageal Cancer |
title_sort |
characterization of the esophageal microbiota and prediction of the metabolic pathways involved in esophageal cancer |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2020-06-01 |
description |
Esophageal microbiota plays important roles in esophageal cancer. Esophagectomy, as the most important therapeutic way, contributes to changes of esophageal microbiome. However, there are few studies examining the esophageal microbiome and the metabolic changes before and after esophagectomy. The present study characterized the esophageal microbiome of 17 patients with esophageal squamous cell carcinoma (ESCC), 11 patients with esophagogastric junction (EGJ) cancer, 15 patients at 9–12 months after radical esophagectomy and 16 healthy controls (HC). 16S ribosomal RNA gene sequencing was used to evaluate the microbiome and predict the metabolic pathways. Our results showed that the microbial diversity was significantly lower in ESCC, EGJ and post-ESCC groups than that in the HC group. The abundance of Fusobacteria was higher (7.01 vs. 1.12%, P = 0.039) and the abundance of Actinobacteria (1.61 vs. 4.04%) was lower in the ESCC group than that in the HC group. We found significant differences in the abundance of Bacteroidetes (20.45 vs. 9.86%, P = 0.026), Fusobacteria (7.01 vs. 1.66%, P = 0.030) between ESCC and post-ESCC groups. The results of microbial composition analysis and PICRUSt demonstrated significant differences between ESCC and HC groups. The β diversity and PICRUSt suggested that the microbial composition and metabolic pathways were similar to HC group after esophagectomy. The monitoring of the esophagus microbiota may be an essential method to predict the recurrence of tumor. |
topic |
microbiota esophageal squamous cell carcinoma esophagectomy 16S ribosomal RNA gene sequencing fusobacteria |
url |
https://www.frontiersin.org/article/10.3389/fcimb.2020.00268/full |
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