Coordination of robust single cell rhythms in the Arabidopsis circadian clock via spatial waves of gene expression
The Arabidopsis circadian clock orchestrates gene regulation across the day/night cycle. Although a multiple feedback loop circuit has been shown to generate the 24-hr rhythm, it remains unclear how robust the clock is in individual cells, or how clock timing is coordinated across the plant. Here we...
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doaj-471394de120b4eee82cc6463b716b1382021-05-05T15:49:31ZengeLife Sciences Publications LtdeLife2050-084X2018-04-01710.7554/eLife.31700Coordination of robust single cell rhythms in the Arabidopsis circadian clock via spatial waves of gene expressionPeter D Gould0Mirela Domijan1https://orcid.org/0000-0003-3853-9119Mark Greenwood2Isao T Tokuda3https://orcid.org/0000-0001-6212-0022Hannah Rees4Laszlo Kozma-Bognar5https://orcid.org/0000-0002-8289-193XAnthony JW Hall6James CW Locke7https://orcid.org/0000-0003-0670-1943Institute of Integrative Biology, University of Liverpool, Liverpool, United KingdomSainsbury Laboratory, University of Cambridge, Cambridge, United Kingdom; Department of Mathematical Sciences, University of Liverpool, Liverpool, United KingdomInstitute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom; Sainsbury Laboratory, University of Cambridge, Cambridge, United Kingdom; Department of Biochemistry, University of Cambridge, Cambridge, United KingdomDepartment of Mechanical Engineering, Ritsumeikan University, Kusatsu, JapanInstitute of Integrative Biology, University of Liverpool, Liverpool, United KingdomBiological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary; Department of Genetics, University of Szeged, Szeged, HungaryInstitute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom; Earlham Institute, Norwich Research Park, Norwich, United KingdomSainsbury Laboratory, University of Cambridge, Cambridge, United Kingdom; Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom; Microsoft Research, Cambridge, United KingdomThe Arabidopsis circadian clock orchestrates gene regulation across the day/night cycle. Although a multiple feedback loop circuit has been shown to generate the 24-hr rhythm, it remains unclear how robust the clock is in individual cells, or how clock timing is coordinated across the plant. Here we examine clock activity at the single cell level across Arabidopsis seedlings over several days under constant environmental conditions. Our data reveal robust single cell oscillations, albeit desynchronised. In particular, we observe two waves of clock activity; one going down, and one up the root. We also find evidence of cell-to-cell coupling of the clock, especially in the root tip. A simple model shows that cell-to-cell coupling and our measured period differences between cells can generate the observed waves. Our results reveal the spatial structure of the plant clock and suggest that unlike the centralised mammalian clock, the Arabidopsis clock has multiple coordination points.https://elifesciences.org/articles/31700single celltime-lapse microscopycircadian clockcell-to-cell coupling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Peter D Gould Mirela Domijan Mark Greenwood Isao T Tokuda Hannah Rees Laszlo Kozma-Bognar Anthony JW Hall James CW Locke |
spellingShingle |
Peter D Gould Mirela Domijan Mark Greenwood Isao T Tokuda Hannah Rees Laszlo Kozma-Bognar Anthony JW Hall James CW Locke Coordination of robust single cell rhythms in the Arabidopsis circadian clock via spatial waves of gene expression eLife single cell time-lapse microscopy circadian clock cell-to-cell coupling |
author_facet |
Peter D Gould Mirela Domijan Mark Greenwood Isao T Tokuda Hannah Rees Laszlo Kozma-Bognar Anthony JW Hall James CW Locke |
author_sort |
Peter D Gould |
title |
Coordination of robust single cell rhythms in the Arabidopsis circadian clock via spatial waves of gene expression |
title_short |
Coordination of robust single cell rhythms in the Arabidopsis circadian clock via spatial waves of gene expression |
title_full |
Coordination of robust single cell rhythms in the Arabidopsis circadian clock via spatial waves of gene expression |
title_fullStr |
Coordination of robust single cell rhythms in the Arabidopsis circadian clock via spatial waves of gene expression |
title_full_unstemmed |
Coordination of robust single cell rhythms in the Arabidopsis circadian clock via spatial waves of gene expression |
title_sort |
coordination of robust single cell rhythms in the arabidopsis circadian clock via spatial waves of gene expression |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2018-04-01 |
description |
The Arabidopsis circadian clock orchestrates gene regulation across the day/night cycle. Although a multiple feedback loop circuit has been shown to generate the 24-hr rhythm, it remains unclear how robust the clock is in individual cells, or how clock timing is coordinated across the plant. Here we examine clock activity at the single cell level across Arabidopsis seedlings over several days under constant environmental conditions. Our data reveal robust single cell oscillations, albeit desynchronised. In particular, we observe two waves of clock activity; one going down, and one up the root. We also find evidence of cell-to-cell coupling of the clock, especially in the root tip. A simple model shows that cell-to-cell coupling and our measured period differences between cells can generate the observed waves. Our results reveal the spatial structure of the plant clock and suggest that unlike the centralised mammalian clock, the Arabidopsis clock has multiple coordination points. |
topic |
single cell time-lapse microscopy circadian clock cell-to-cell coupling |
url |
https://elifesciences.org/articles/31700 |
work_keys_str_mv |
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