Molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients
Objective: To describe the genotypic variability of group A rotavirus (RVA) found in immunosuppressed and non-immunosuppressed pediatric patients treated at the Hospital de Clínicas da Universidade Federal do Paraná (HC-UFPR), Curitiba, Paraná. Methods: A cross-sectional study was conducted with 1,1...
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doaj-471be3e875ac4c84b0e77e01323641962020-11-24T20:59:53ZporElsevierJornal de Pediatria (Versão em Português)2255-55362013-05-0189327828510.1016/j.jpedp.2012.11.007Molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patientsLuciane A. Pereira0Carla E.O. Ferreira1Giovana D. Turchetto2Meri B. Nogueira3Luine R. Vidal4Cristina R. Cruz5Maria C. Debur6Sergio M. De Almeida7Sonia M. Raboni8Bióloga, Laboratório de Virologia, Universidade Federal do Paraná (UFPR), Curitiba, PR, BrasilBióloga, Laboratório de Virologia, Universidade Federal do Paraná (UFPR), Curitiba, PR, BrasilBioquímica, Laboratório de Virologia, UFPR, Curitiba, PR, BrasilBioquímica, Laboratório de Virologia, UFPR, Curitiba, PR, BrasilBioquímica, Laboratório de Virologia, UFPR, Curitiba, PR, BrasilProfessora, Departamento de Pediatria, UFPR, Curitiba, PR, BrasilBioquímica, Laboratório de Saúde Pública do Estado, Secretaria Estadual de Saúde do Paraná, Curitiba, PR, BrasilMédico, Laboratório de Virologia, UFPR, Curitiba, PR, BrasilProfessora-associada, Laboratório de Virologia e Departamento de Doenças Infecciosas, UFPR, Curitiba, PR, BrasilObjective: To describe the genotypic variability of group A rotavirus (RVA) found in immunosuppressed and non-immunosuppressed pediatric patients treated at the Hospital de Clínicas da Universidade Federal do Paraná (HC-UFPR), Curitiba, Paraná. Methods: A cross-sectional study was conducted with 1,140 stool samples collected from April, 2001 to December, 2008 in outpatients and hospitalized patients with acute gastroenteritis referred to the hospital. RVA diagnosis was performed through the latex agglutination method and enzyme immunoassay. Reverse transcription followed by multiplex hemi-nested polymerase chain reaction (PCR) and nucleotide sequencing were used for genotype characterization. Genotype combinations, clinical data, epidemiological data, laboratory data, and presence of hospital-acquired infections were reported. Results: A total of 80 rotavirus-positive stool samples were analyzed. The most frequent associations between genotypes G and P were: G4 P[8] (38.9%), G1 P[8] (30.5%), G9 P[8] (13.9%), G2 P[4] (6.9%), and G3 P[8] (1.4%). G2 P[4] was the most prevalent genotype after the vaccine implementation in the years 2006 and 2008. A total of 62.5% of children aged less than 12 months were found to be infected. Of these, 55.6% had severe dehydration and 26.7% needed intensive care. A frequency of 12.5% of nosocomial infections was found. No correlation was observed between genotype and severity of infection in the study patients. Conclusion: RVA infections can be associated with severe clinical manifestations, and the surveillance of genotypic variability of this virus is crucial to monitor the emergence of new strains and the impact of the immunization in these patients.http://www.sciencedirect.com/science/article/pii/S2255553613000542GastroenteritisGenotypesPediatric infectionsRotavirus |
collection |
DOAJ |
language |
Portuguese |
format |
Article |
sources |
DOAJ |
author |
Luciane A. Pereira Carla E.O. Ferreira Giovana D. Turchetto Meri B. Nogueira Luine R. Vidal Cristina R. Cruz Maria C. Debur Sergio M. De Almeida Sonia M. Raboni |
spellingShingle |
Luciane A. Pereira Carla E.O. Ferreira Giovana D. Turchetto Meri B. Nogueira Luine R. Vidal Cristina R. Cruz Maria C. Debur Sergio M. De Almeida Sonia M. Raboni Molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients Jornal de Pediatria (Versão em Português) Gastroenteritis Genotypes Pediatric infections Rotavirus |
author_facet |
Luciane A. Pereira Carla E.O. Ferreira Giovana D. Turchetto Meri B. Nogueira Luine R. Vidal Cristina R. Cruz Maria C. Debur Sergio M. De Almeida Sonia M. Raboni |
author_sort |
Luciane A. Pereira |
title |
Molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients |
title_short |
Molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients |
title_full |
Molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients |
title_fullStr |
Molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients |
title_full_unstemmed |
Molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients |
title_sort |
molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients |
publisher |
Elsevier |
series |
Jornal de Pediatria (Versão em Português) |
issn |
2255-5536 |
publishDate |
2013-05-01 |
description |
Objective: To describe the genotypic variability of group A rotavirus (RVA) found in immunosuppressed and non-immunosuppressed pediatric patients treated at the Hospital de Clínicas da Universidade Federal do Paraná (HC-UFPR), Curitiba, Paraná.
Methods: A cross-sectional study was conducted with 1,140 stool samples collected from April, 2001 to December, 2008 in outpatients and hospitalized patients with acute gastroenteritis referred to the hospital. RVA diagnosis was performed through the latex agglutination method and enzyme immunoassay. Reverse transcription followed by multiplex hemi-nested polymerase chain reaction (PCR) and nucleotide sequencing were used for genotype characterization. Genotype combinations, clinical data, epidemiological data, laboratory data, and presence of hospital-acquired infections were reported.
Results: A total of 80 rotavirus-positive stool samples were analyzed. The most frequent associations between genotypes G and P were: G4 P[8] (38.9%), G1 P[8] (30.5%), G9 P[8] (13.9%), G2 P[4] (6.9%), and G3 P[8] (1.4%). G2 P[4] was the most prevalent genotype after the vaccine implementation in the years 2006 and 2008. A total of 62.5% of children aged less than 12 months were found to be infected. Of these, 55.6% had severe dehydration and 26.7% needed intensive care. A frequency of 12.5% of nosocomial infections was found. No correlation was observed between genotype and severity of infection in the study patients.
Conclusion: RVA infections can be associated with severe clinical manifestations, and the surveillance of genotypic variability of this virus is crucial to monitor the emergence of new strains and the impact of the immunization in these patients. |
topic |
Gastroenteritis Genotypes Pediatric infections Rotavirus |
url |
http://www.sciencedirect.com/science/article/pii/S2255553613000542 |
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