Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS.
Antibiotic resistance is a major public health problem requiring the early optimization of antibiotic prescriptions. Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS) has been shown to accurately identify bacteria from positive blood culture. Here, we develo...
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doaj-4722972780a64407920d232a1becf0da2020-11-24T22:12:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011310e020560310.1371/journal.pone.0205603Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS.Marlène SaugetXavier BertrandDidier HocquetAntibiotic resistance is a major public health problem requiring the early optimization of antibiotic prescriptions. Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS) has been shown to accurately identify bacteria from positive blood culture. Here, we developed a rapid detection of Escherichia coli resistance to amoxicillin (AMX) and cefotaxime (CTX) from positive blood culture based on MALDI-TOF MS. Potential sparing of broad-spectrum antibiotics was also evaluated. We tested 103 E. coli-positive blood cultures. Blood cultures were subculture 1-hour in antibiotic-free rich liquid media before further incubation with and without AMX for 2.5 h or CTX for 2 h. Protein extracts associated with an internal control were spotted on a MALDI-TOF target and spectra were analyzed with the MBT-ASTRA prototype software (Bruker Daltonik GmbH, Bremen, Germany). Bacterial growth ratio was calculated from the AUC spectra obtained in the presence and absence of the antibiotic and compared to a threshold which classified E. coli as susceptible or resistant. Results were interpreted with MICs determined using agar dilution method as reference technique. MBT-ASTRA recognized 95% and 84% of the AMX- and CTX-susceptible isolates, respectively. Overall, quantitative analysis of mass spectra allows susceptibility testing within 4 hours after the positivity of blood culture with E. coli. At the first report of positive blood culture, MALDI-TOF MS would then provide the prescribers with the bacterial identification and the susceptibility to AMX and CTX, thus limiting the use of broad-spectrum compounds.http://europepmc.org/articles/PMC6181389?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marlène Sauget Xavier Bertrand Didier Hocquet |
spellingShingle |
Marlène Sauget Xavier Bertrand Didier Hocquet Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS. PLoS ONE |
author_facet |
Marlène Sauget Xavier Bertrand Didier Hocquet |
author_sort |
Marlène Sauget |
title |
Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS. |
title_short |
Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS. |
title_full |
Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS. |
title_fullStr |
Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS. |
title_full_unstemmed |
Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS. |
title_sort |
rapid antibiotic susceptibility testing on blood cultures using maldi-tof ms. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2018-01-01 |
description |
Antibiotic resistance is a major public health problem requiring the early optimization of antibiotic prescriptions. Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS) has been shown to accurately identify bacteria from positive blood culture. Here, we developed a rapid detection of Escherichia coli resistance to amoxicillin (AMX) and cefotaxime (CTX) from positive blood culture based on MALDI-TOF MS. Potential sparing of broad-spectrum antibiotics was also evaluated. We tested 103 E. coli-positive blood cultures. Blood cultures were subculture 1-hour in antibiotic-free rich liquid media before further incubation with and without AMX for 2.5 h or CTX for 2 h. Protein extracts associated with an internal control were spotted on a MALDI-TOF target and spectra were analyzed with the MBT-ASTRA prototype software (Bruker Daltonik GmbH, Bremen, Germany). Bacterial growth ratio was calculated from the AUC spectra obtained in the presence and absence of the antibiotic and compared to a threshold which classified E. coli as susceptible or resistant. Results were interpreted with MICs determined using agar dilution method as reference technique. MBT-ASTRA recognized 95% and 84% of the AMX- and CTX-susceptible isolates, respectively. Overall, quantitative analysis of mass spectra allows susceptibility testing within 4 hours after the positivity of blood culture with E. coli. At the first report of positive blood culture, MALDI-TOF MS would then provide the prescribers with the bacterial identification and the susceptibility to AMX and CTX, thus limiting the use of broad-spectrum compounds. |
url |
http://europepmc.org/articles/PMC6181389?pdf=render |
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