Engineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates

Engineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates

Abstract Background Biosynthesis of steroidal drugs is of great benefit in pharmaceutical manufacturing as the process involves efficient enzymatic catalysis at ambient temperature and atmospheric pressure compared to chemical synthesis. 3-ketosteroid-∆1-dehydrogenase from Arthrobacter simplex (KsdD...

Full description

Bibliographic Details
Main Authors:	Shuhong Mao, Jian-Wen Wang, Fufeng Liu, Zhangliang Zhu, Dengke Gao, Qianqian Guo, Panpan Xu, Zheng Ma, Yali Hou, Xiaotao Cheng, Dengyue Sun, Fuping Lu, Hui-Min Qin
Format:	Article
Language:	English
Published:	BMC 2018-09-01
Series:	Microbial Cell Factories
Subjects:	Dehydrogenase Saturation mutagenesis Rational design Steered molecular dynamics 3-ketosteroid
Online Access:	http://link.springer.com/article/10.1186/s12934-018-0981-0

Similar Items

Universal capability of 3-ketosteroid Δ1-dehydrogenases to catalyze Δ1-dehydrogenation of C17-substituted steroids
by: Patrycja Wójcik, et al.
Published: (2021-06-01)

Identification, function, and application of 3-ketosteroid Δ1-dehydrogenase isozymes in Mycobacterium neoaurum DSM 1381 for the production of steroidic synthons
by: Ruijie Zhang, et al.
Published: (2018-05-01)

Expression and purification of the p75 neurotrophin receptor transmembrane domain using a ketosteroid isomerase tag
by: Li Qingxin, et al.
Published: (2012-04-01)

Further Studies on the 3-Ketosteroid 9α-Hydroxylase of <i>Rhodococcus ruber</i> Chol-4, a Rieske Oxygenase of the Steroid Degradation Pathway
by: Sara Baldanta, et al.
Published: (2021-05-01)

Application of Immobilized Cholest-4-En-3-One Δ<sup>1</sup>-Dehydrogenase from Sterolibacterium Denitrificans for Dehydrogenation of Steroids
by: Mateusz Tataruch, et al.
Published: (2020-12-01)

Probing the Role of the Conserved Arg174 in Formate Dehydrogenase by Chemical Modification and Site-Directed Mutagenesis
by: Mohammed Hamed Alqarni, et al.
Published: (2021-02-01)

Crystallization and mutational studies of carbon monoxide dehydrogenase from moorella thermoacetica
by: Kim, Eun Jin
Published: (2004)

Mechanistic, inhibitory, and mutagenic studies of inositol dehydrogenase from <i>Bacillus subtilis</i>
by: Zheng, Hongyan
Published: (2010)

Promoter analysis by saturation mutagenesis
by: Baliga Nitin
Published: (2001-01-01)

Construction of Mutant Glucose Oxidases with Increased Dye-Mediated Dehydrogenase Activity
by: Koji Sode, et al.
Published: (2012-11-01)

Hijacking Transposable Elements for Saturation Mutagenesis in Fungi
by: Sanne Schrevens, et al.
Published: (2021-04-01)

Serum lipids and dehydroepiandrosterone excretion in normal subjects
by: Jiří Šonka, et al.
Published: (1968-11-01)

Determinants Of Globular Protein Stability And Temperature Sensitivity Inferred From Saturation Mutagenesis Of CcdB
by: Bajaj, Kanika
Published: (2011)

Production of 4-Ene-3-ketosteroids in Corynebacterium glutamicum
by: Julia García-Fernández, et al.
Published: (2017-10-01)

Saturation Mutagenesis for Phenylalanine Ammonia Lyases of Enhanced Catalytic Properties
by: Raluca Bianca Tomoiagă, et al.
Published: (2020-05-01)

Enhanced Hydrogen Production in Escherichia coli Through Chemical Mutagenesis, Gene Deletion, and Transposon Mutagenesis
by: Garzon Sanabria, Andrea Juliana
Published: (2011)

Mutagenesis of human lymphoblast cells
by: Kotek, Joseph John
Published: (2011)

Site Directed Mutagenesis Of Dienelactone Hydrolase
by: Chen, Wei, 1965-
Published: (1992)

Mutagenesis in Lotus corniculatus L.
by: Therrien, Mario C.
Published: (1981)

Prediction of Function Determining and Buried Residues Through Analysis of Saturation Mutagenesis Datasets
by: Munmun Bhasin, et al.
Published: (2021-03-01)

Substrate Inhibition of 5β-Δ4-3-Ketosteroid Dehydrogenase in Sphingobium sp. Strain Chol11 Acts as Circuit Breaker During Growth With Toxic Bile Salts
by: Franziska M. Feller, et al.
Published: (2021-03-01)

Site directed mutagenesis and kinetic study of ferrochelatase from chironomidae larvae.
Published: (2004)

Functional Analysis of 3-Dehydroquinate Dehydratase/Shikimate Dehydrogenases Involved in Shikimate Pathway in Camellia sinensis
by: Keyi Huang, et al.
Published: (2019-10-01)

Improvement of Biocatalysts for Industrial and Environmental Purposes by Saturation Mutagenesis
by: Francesca Valetti, et al.
Published: (2013-10-01)

17β-Hydroxysteroid dehydrogenases/17-ketosteroid reductases (17HSD/KSRs) in prostate cancer:the role of 17HSD/KSR types 2, 5, and 7 in steroid hormone action and loss of heterozygosity at chromosome region 16q
by: Härkönen, P. (Päivi)
Published: (2005)

Site Directed Mutagenesis of β-Ketoadipate Succinyl-Coenzyme A Transferase II from Acinetobacter Calcoaceticus
by: Sheng, Mei
Published: (1993)

High-fat diet enhanced retinal dehydrogenase activity, but suppressed retinol dehydrogenase activity in liver of rats
by: Mian Zhang, et al.
Published: (2015-04-01)

OneClick: A Program for Designing Focused Mutagenesis Experiments
by: Mark Warburton, et al.
Published: (2015-07-01)

Biochemistry and evolution of the shikimate dehydrogenase/quinate dehydrogenase gene family in plants
by: Carrington, Yuriko
Published: (2020)

APOBEC mutagenesis is low in most types of non-B DNA structures
by: Abasov, R., et al.
Published: (2022)

Physical and kinetic properties of dihydroorotate dehydrogenase from Lactobacillus bulgaricus
by: Taylor, Craig David
Published: (1969)

Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9α-hydroxylated derivatives by engineered Mycobacteria
by: Xin Li, et al.
Published: (2021-08-01)

Mutagenesis and antimutagenesis in Big Blue ® lacI transgenic rats
by: Yang, Haiyan
Published: (2018)

Multiple-site fragment deletion, insertion and substitution mutagenesis by modified overlap extension PCR
by: Fanli Zeng, et al.
Published: (2017-03-01)

In vitro Inactivation of Latent HSV by Targeted Mutagenesis Using an HSV-specific Homing Endonuclease
by: Martine Aubert, et al.
Published: (2014-01-01)

Expression and site-directed mutagenesis studies of a ribosome-inactivating protein : neo-trichosanthin
by: Shi, Qingli
Published: (1999)

Construction and characterization of a novel glucose dehydrogenase-leucine dehydrogenase fusion enzyme for the biosynthesis of l-tert-leucine
by: Langxing Liao, et al.
Published: (2021-01-01)

Development of a novel dehydrogenase and a stable cofactor regeneration system
by: Vázquez-Figueroa, Eduardo
Published: (2010)

Isocitrate dehydrogenase, lactate dehydrogenase and α-glycerophosphate dehydrogenase polymorphysm enzyme of Black Tiger Shrimp (Penaeus monodon Fab.) which is hydrogen sulfide resistant
by: OKID PARAMA ASTIRIN, et al.
Published: (2006-07-01)

The in vivo role of Rev1 in mutagenesis and carcinogenesis
by: Megumi Sasatani, et al.
Published: (2020-02-01)