Immunomodulatory effect of the hydroalcoholic extract of Abrus precatorius L. leaves against cyclophosphamide-induced immunosuppression in mice
This study presents the immunomodulatory potential of Abrus precatorius Linn. (Indian wild licorice) leaves. A hydroalcoholic extract of A. precatorius leaves (EAPL) was prepared by maceration. Thirty male mice were divided into five groups as follows: control group, model control group (cyclophosph...
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University of Belgrade, University of Novi Sad
2021-01-01
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doaj-4743977cb90b438baedbd0e620bc373e2021-07-19T07:08:31ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392021-01-0173227929010.2298/ABS210219022M0354-46642100022MImmunomodulatory effect of the hydroalcoholic extract of Abrus precatorius L. leaves against cyclophosphamide-induced immunosuppression in miceModi Chirag M.0Bhatt Punit R.1Patel Urvesh D.2Patel Harshad B.3Pandya Kajal B.4Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, Gujarat, IndiaDepartment of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, Gujarat, IndiaDepartment of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, Gujarat, IndiaDepartment of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, Gujarat, IndiaDepartment of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, Gujarat, IndiaThis study presents the immunomodulatory potential of Abrus precatorius Linn. (Indian wild licorice) leaves. A hydroalcoholic extract of A. precatorius leaves (EAPL) was prepared by maceration. Thirty male mice were divided into five groups as follows: control group, model control group (cyclophosphamide-treated), and three treatment groups (treated with EAPL at doses of 100, 200 and 300 mg/kg, per os, daily for 14 days). Parameters, including hematological, biochemical, organ indices, hemagglutination test (HA titer), delayed-type hypersensitivity (DTH), interleukin-2 (IL-2) level, splenocyte proliferation and liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) analysis were evaluated. Histopathological examination was carried out for the spleen, kidney and liver. Cyclophosphamide (CPMD)-induced changes in white blood cells, lymphocytes and platelets were improved in the treatment groups. Total protein and albumin levels in the treatment groups were significantly higher. EAPL treatment significantly stimulated splenic T-lymphocytemediated proliferation. Neutrophil adhesion was insignificantly decreased in the model control group compared to the normal control group, which was slightly improved by EAPL treatment. EAPL treatment significantly improved the HA titer and cell-mediated immunity, which is an indication of antibody production. The IL-2 level was significantly higher in the treatment groups that received 200 and 300 mg/kg EAPL. LC-QTOF-MS analysis of EAPL showed the presence of flavonoids, lignans, iridoids and phenolic glycosides. These results suggest that A. precatorius leaves are a good candidate for a new immunomodulatory herbal formulation.http://www.doiserbia.nb.rs/img/doi/0354-4664/2021/0354-46642100022M.pdfabrus precatoriusimmunomodulationcyclophosphamideimmunitylc-qtof-ms |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Modi Chirag M. Bhatt Punit R. Patel Urvesh D. Patel Harshad B. Pandya Kajal B. |
spellingShingle |
Modi Chirag M. Bhatt Punit R. Patel Urvesh D. Patel Harshad B. Pandya Kajal B. Immunomodulatory effect of the hydroalcoholic extract of Abrus precatorius L. leaves against cyclophosphamide-induced immunosuppression in mice Archives of Biological Sciences abrus precatorius immunomodulation cyclophosphamide immunity lc-qtof-ms |
author_facet |
Modi Chirag M. Bhatt Punit R. Patel Urvesh D. Patel Harshad B. Pandya Kajal B. |
author_sort |
Modi Chirag M. |
title |
Immunomodulatory effect of the hydroalcoholic extract of Abrus precatorius L. leaves against cyclophosphamide-induced immunosuppression in mice |
title_short |
Immunomodulatory effect of the hydroalcoholic extract of Abrus precatorius L. leaves against cyclophosphamide-induced immunosuppression in mice |
title_full |
Immunomodulatory effect of the hydroalcoholic extract of Abrus precatorius L. leaves against cyclophosphamide-induced immunosuppression in mice |
title_fullStr |
Immunomodulatory effect of the hydroalcoholic extract of Abrus precatorius L. leaves against cyclophosphamide-induced immunosuppression in mice |
title_full_unstemmed |
Immunomodulatory effect of the hydroalcoholic extract of Abrus precatorius L. leaves against cyclophosphamide-induced immunosuppression in mice |
title_sort |
immunomodulatory effect of the hydroalcoholic extract of abrus precatorius l. leaves against cyclophosphamide-induced immunosuppression in mice |
publisher |
University of Belgrade, University of Novi Sad |
series |
Archives of Biological Sciences |
issn |
0354-4664 1821-4339 |
publishDate |
2021-01-01 |
description |
This study presents the immunomodulatory potential of Abrus precatorius Linn. (Indian wild licorice) leaves. A hydroalcoholic extract of A. precatorius leaves (EAPL) was prepared by maceration. Thirty male mice were divided into five groups as follows: control group, model control group (cyclophosphamide-treated), and three treatment groups (treated with EAPL at doses of 100, 200 and 300 mg/kg, per os, daily for 14 days). Parameters, including hematological, biochemical, organ indices, hemagglutination test (HA titer), delayed-type hypersensitivity (DTH), interleukin-2 (IL-2) level, splenocyte proliferation and liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) analysis were evaluated. Histopathological examination was carried out for the spleen, kidney and liver. Cyclophosphamide (CPMD)-induced changes in white blood cells, lymphocytes and platelets were improved in the treatment groups. Total protein and albumin levels in the treatment groups were significantly higher. EAPL treatment significantly stimulated splenic T-lymphocytemediated proliferation. Neutrophil adhesion was insignificantly decreased in the model control group compared to the normal control group, which was slightly improved by EAPL treatment. EAPL treatment significantly improved the HA titer and cell-mediated immunity, which is an indication of antibody production. The IL-2 level was significantly higher in the treatment groups that received 200 and 300 mg/kg EAPL. LC-QTOF-MS analysis of EAPL showed the presence of flavonoids, lignans, iridoids and phenolic glycosides. These results suggest that A. precatorius leaves are a good candidate for a new immunomodulatory herbal formulation. |
topic |
abrus precatorius immunomodulation cyclophosphamide immunity lc-qtof-ms |
url |
http://www.doiserbia.nb.rs/img/doi/0354-4664/2021/0354-46642100022M.pdf |
work_keys_str_mv |
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