Morphological and morphometric differentiation of dorsal-spined first stage larvae of lungworms (Nematoda: Protostrongylidae) infecting muskoxen (Ovibos moschatus) in the central Canadian Arctic
Umingmakstrongylus pallikuukensis and Varestrongylus eleguneniensis are the two most common protostrongylid nematodes infecting muskoxen in the North American Arctic and Subarctic. First stage larvae (L1) of these lungworms have considerable morphological similarity that makes their differential dia...
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doaj-474e5b1dc1d54aa08f9f2234dbe2f1c72020-11-24T21:43:30ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442015-12-014328329010.1016/j.ijppaw.2015.05.003Morphological and morphometric differentiation of dorsal-spined first stage larvae of lungworms (Nematoda: Protostrongylidae) infecting muskoxen (Ovibos moschatus) in the central Canadian ArcticPratap Kafle0Manigandan Lejeune1Guilherme G. Verocai2Eric P. Hoberg3Susan J. Kutz4Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, CanadaCanadian Wildlife Health Cooperative – Alberta Node, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, CanadaDepartment of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, CanadaU.S. National Parasite Collection, Animal Parasitic Diseases Laboratory, USDA, ARS, BARC East 1180, 10300 Baltimore Avenue, Beltsville, MD 20705, USADepartment of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, CanadaUmingmakstrongylus pallikuukensis and Varestrongylus eleguneniensis are the two most common protostrongylid nematodes infecting muskoxen in the North American Arctic and Subarctic. First stage larvae (L1) of these lungworms have considerable morphological similarity that makes their differential diagnosis very difficult. Using light microscopy, we studied in detail the L1 of these two species and identified the key differences in morphological and morphometric attributes. Thirty L1 of each species from naturally infected muskox were heat-killed and then assessed for morphological and morphometric features that could be used for species-level differentiation. Key differentiating features include: length and morphology of the tail extension, curvature of the body, ventral post-anal transverse cuticular striations, and total body length. A laboratory guide for differentiation of L1 based on these species-specific characters was prepared and used by an experienced observer to identify an additional 35 L1 extracted from a different set of fecal samples from free-ranging muskoxen with mixed infections. The identities of these L1 were confirmed by sequence analysis of the ITS-2 region of the nuclear ribosomal DNA. Accuracy of morphological identification was 100 percent, reflecting the reliability of the proposed guide for differentiation. Using the guide, three minimally trained lab assistants each fixed and accurately identified 10 of 10 randomly selected L1. Ability to morphologically differentiate these facilitates the monitoring of overlapping range expansion of both parasites in the Canadian Arctic. Studies enabling species-level parasite identification are also critical for defining biodiversity, detecting mixed infections, and understanding host–parasite interactions. Morphological identification is a simple, reliable and cost-effective alternative to labor and equipment intensive molecular methods and can easily be performed in low resource settings.http://www.sciencedirect.com/science/article/pii/S2213224415000267Arctic parasitologyDiagnostic parasitologyMorphology and morphometryUmingmakstrongylus pallikuukensisVarestrongylus eleguneniensis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pratap Kafle Manigandan Lejeune Guilherme G. Verocai Eric P. Hoberg Susan J. Kutz |
spellingShingle |
Pratap Kafle Manigandan Lejeune Guilherme G. Verocai Eric P. Hoberg Susan J. Kutz Morphological and morphometric differentiation of dorsal-spined first stage larvae of lungworms (Nematoda: Protostrongylidae) infecting muskoxen (Ovibos moschatus) in the central Canadian Arctic International Journal for Parasitology: Parasites and Wildlife Arctic parasitology Diagnostic parasitology Morphology and morphometry Umingmakstrongylus pallikuukensis Varestrongylus eleguneniensis |
author_facet |
Pratap Kafle Manigandan Lejeune Guilherme G. Verocai Eric P. Hoberg Susan J. Kutz |
author_sort |
Pratap Kafle |
title |
Morphological and morphometric differentiation of dorsal-spined first stage larvae of lungworms (Nematoda: Protostrongylidae) infecting muskoxen (Ovibos moschatus) in the central Canadian Arctic |
title_short |
Morphological and morphometric differentiation of dorsal-spined first stage larvae of lungworms (Nematoda: Protostrongylidae) infecting muskoxen (Ovibos moschatus) in the central Canadian Arctic |
title_full |
Morphological and morphometric differentiation of dorsal-spined first stage larvae of lungworms (Nematoda: Protostrongylidae) infecting muskoxen (Ovibos moschatus) in the central Canadian Arctic |
title_fullStr |
Morphological and morphometric differentiation of dorsal-spined first stage larvae of lungworms (Nematoda: Protostrongylidae) infecting muskoxen (Ovibos moschatus) in the central Canadian Arctic |
title_full_unstemmed |
Morphological and morphometric differentiation of dorsal-spined first stage larvae of lungworms (Nematoda: Protostrongylidae) infecting muskoxen (Ovibos moschatus) in the central Canadian Arctic |
title_sort |
morphological and morphometric differentiation of dorsal-spined first stage larvae of lungworms (nematoda: protostrongylidae) infecting muskoxen (ovibos moschatus) in the central canadian arctic |
publisher |
Elsevier |
series |
International Journal for Parasitology: Parasites and Wildlife |
issn |
2213-2244 |
publishDate |
2015-12-01 |
description |
Umingmakstrongylus pallikuukensis and Varestrongylus eleguneniensis are the two most common protostrongylid nematodes infecting muskoxen in the North American Arctic and Subarctic. First stage larvae (L1) of these lungworms have considerable morphological similarity that makes their differential diagnosis very difficult. Using light microscopy, we studied in detail the L1 of these two species and identified the key differences in morphological and morphometric attributes. Thirty L1 of each species from naturally infected muskox were heat-killed and then assessed for morphological and morphometric features that could be used for species-level differentiation. Key differentiating features include: length and morphology of the tail extension, curvature of the body, ventral post-anal transverse cuticular striations, and total body length. A laboratory guide for differentiation of L1 based on these species-specific characters was prepared and used by an experienced observer to identify an additional 35 L1 extracted from a different set of fecal samples from free-ranging muskoxen with mixed infections. The identities of these L1 were confirmed by sequence analysis of the ITS-2 region of the nuclear ribosomal DNA. Accuracy of morphological identification was 100 percent, reflecting the reliability of the proposed guide for differentiation. Using the guide, three minimally trained lab assistants each fixed and accurately identified 10 of 10 randomly selected L1. Ability to morphologically differentiate these facilitates the monitoring of overlapping range expansion of both parasites in the Canadian Arctic. Studies enabling species-level parasite identification are also critical for defining biodiversity, detecting mixed infections, and understanding host–parasite interactions. Morphological identification is a simple, reliable and cost-effective alternative to labor and equipment intensive molecular methods and can easily be performed in low resource settings. |
topic |
Arctic parasitology Diagnostic parasitology Morphology and morphometry Umingmakstrongylus pallikuukensis Varestrongylus eleguneniensis |
url |
http://www.sciencedirect.com/science/article/pii/S2213224415000267 |
work_keys_str_mv |
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