In Vitro Study of Action Mode of Rhodotorula minuta Dmg 16 BEP as Biocontrol Agents on Alternaria solani
Rhodotorula sp. is widely known as a biocontrol agent and is reported effective in controlling several diseases on crops. Rhodotorula minuta Dmg 16 BEP is an antagonist yeast from Indonesia which was effective in controlling various plant diseases. The objective of this research was to study the in...
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Universitas Gadjah Mada
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doaj-4777f463945246d990b6279bc285a4332020-11-25T03:14:14ZengUniversitas Gadjah MadaJurnal Perlindungan Tanaman Indonesia1410-16372548-47882020-07-01241283310.22146/jpti.4334428216In Vitro Study of Action Mode of Rhodotorula minuta Dmg 16 BEP as Biocontrol Agents on Alternaria solaniWawan Setiawan0Suryo Wiyono1Efi Toding Tondok2Atit Kanti3I Made Sudiana4Graduate Study Program of Phytopathology, Faculty of Agriculture, IPB University Jln. Raya Dramaga, Babakan Raya 3, Dramaga, Bogor, West Java 16680Department of Plant Protection, Faculty of Agriculture, IPB University Jln. Raya Dramaga, Kampus IPB Dramaga, Bogor, West Java 16680Department of Plant Protection, Faculty of Agriculture, IPB University Jln. Raya Dramaga, Kampus IPB Dramaga, Bogor, West Java 16680Research Center for Biology, Indonesian Institute of Sciences Cibinong Jln. Raya Jakarta-Bogor KM 46, Cibinong, West JavaResearch Center for Biology, Indonesian Institute of Sciences Cibinong Jln. Raya Jakarta-Bogor KM 46, Cibinong, West JavaRhodotorula sp. is widely known as a biocontrol agent and is reported effective in controlling several diseases on crops. Rhodotorula minuta Dmg 16 BEP is an antagonist yeast from Indonesia which was effective in controlling various plant diseases. The objective of this research was to study the in vitro mode of action of R. minuta Dmg 16 BEP against Alternaria solani. The antibiosis ability of R. minuta Dmg 16 BEP to A. solani might be based on the activity of volatile compounds and not from its metabolites products. The growth of A. solani in paper disk assay and dual culture tests were not inhibited nor inhibitory zones developed, whereas the inverse petridish test showed the growth inhibition of A. solani. The results of the chitinolytic activity test on chitin agar and proteolytic on skim milk agar showed that no clear zone was formed. R. minuta has a strong hyperparasitic ability according to the slide culture test as the yeast cells surrounded A. solani hyphae and caused damage to some parts of hyphae.https://jurnal.ugm.ac.id/jpti/article/view/43344alternaria solaniantibiosishyperparasitismr. minuta |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wawan Setiawan Suryo Wiyono Efi Toding Tondok Atit Kanti I Made Sudiana |
spellingShingle |
Wawan Setiawan Suryo Wiyono Efi Toding Tondok Atit Kanti I Made Sudiana In Vitro Study of Action Mode of Rhodotorula minuta Dmg 16 BEP as Biocontrol Agents on Alternaria solani Jurnal Perlindungan Tanaman Indonesia alternaria solani antibiosis hyperparasitism r. minuta |
author_facet |
Wawan Setiawan Suryo Wiyono Efi Toding Tondok Atit Kanti I Made Sudiana |
author_sort |
Wawan Setiawan |
title |
In Vitro Study of Action Mode of Rhodotorula minuta Dmg 16 BEP as Biocontrol Agents on Alternaria solani |
title_short |
In Vitro Study of Action Mode of Rhodotorula minuta Dmg 16 BEP as Biocontrol Agents on Alternaria solani |
title_full |
In Vitro Study of Action Mode of Rhodotorula minuta Dmg 16 BEP as Biocontrol Agents on Alternaria solani |
title_fullStr |
In Vitro Study of Action Mode of Rhodotorula minuta Dmg 16 BEP as Biocontrol Agents on Alternaria solani |
title_full_unstemmed |
In Vitro Study of Action Mode of Rhodotorula minuta Dmg 16 BEP as Biocontrol Agents on Alternaria solani |
title_sort |
in vitro study of action mode of rhodotorula minuta dmg 16 bep as biocontrol agents on alternaria solani |
publisher |
Universitas Gadjah Mada |
series |
Jurnal Perlindungan Tanaman Indonesia |
issn |
1410-1637 2548-4788 |
publishDate |
2020-07-01 |
description |
Rhodotorula sp. is widely known as a biocontrol agent and is reported effective in controlling several diseases on crops. Rhodotorula minuta Dmg 16 BEP is an antagonist yeast from Indonesia which was effective in controlling various plant diseases. The objective of this research was to study the in vitro mode of action of R. minuta Dmg 16 BEP against Alternaria solani. The antibiosis ability of R. minuta Dmg 16 BEP to A. solani might be based on the activity of volatile compounds and not from its metabolites products. The growth of A. solani in paper disk assay and dual culture tests were not inhibited nor inhibitory zones developed, whereas the inverse petridish test showed the growth inhibition of A. solani. The results of the chitinolytic activity test on chitin agar and proteolytic on skim milk agar showed that no clear zone was formed. R. minuta has a strong hyperparasitic ability according to the slide culture test as the yeast cells surrounded A. solani hyphae and caused damage to some parts of hyphae. |
topic |
alternaria solani antibiosis hyperparasitism r. minuta |
url |
https://jurnal.ugm.ac.id/jpti/article/view/43344 |
work_keys_str_mv |
AT wawansetiawan invitrostudyofactionmodeofrhodotorulaminutadmg16bepasbiocontrolagentsonalternariasolani AT suryowiyono invitrostudyofactionmodeofrhodotorulaminutadmg16bepasbiocontrolagentsonalternariasolani AT efitodingtondok invitrostudyofactionmodeofrhodotorulaminutadmg16bepasbiocontrolagentsonalternariasolani AT atitkanti invitrostudyofactionmodeofrhodotorulaminutadmg16bepasbiocontrolagentsonalternariasolani AT imadesudiana invitrostudyofactionmodeofrhodotorulaminutadmg16bepasbiocontrolagentsonalternariasolani |
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