Cytotoxicity of Self-Adhesive Resin Cements on Human Periodontal Ligament Fibroblasts
The aim of this study was to evaluate the potential cytotoxicity of self-adhesive resin cements with or without light irradiation on human periodontal ligament fibroblasts (HPDLFs) in vitro. Three self-adhesive resin cements (RelyX U200, Maxcem Elite and Multilink Speed) were cured with light or not...
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2018-01-01
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Online Access: | http://dx.doi.org/10.1155/2018/7823467 |
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doaj-479af25482df4d4d9145180cbf4dba002020-11-24T22:01:46ZengHindawi LimitedBioMed Research International2314-61332314-61412018-01-01201810.1155/2018/78234677823467Cytotoxicity of Self-Adhesive Resin Cements on Human Periodontal Ligament FibroblastsFangfang Sun0Ying Liu1Yahui Pan2Meng Chen3Xiangfeng Meng4Department of Prosthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Stomatology, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Prosthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Respiratory, Children’s Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Prosthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaThe aim of this study was to evaluate the potential cytotoxicity of self-adhesive resin cements with or without light irradiation on human periodontal ligament fibroblasts (HPDLFs) in vitro. Three self-adhesive resin cements (RelyX U200, Maxcem Elite and Multilink Speed) were cured with light or not. Cured cements were stored at 37°C for 24 h in water or Dulbecco’s modified eagle medium (DMEM) medium. Their chromatographic analysis of water-based extract solution was made and then the DMEM-based extract solution was diluted in complete DMEM {1:5, 1:10, 1:20, 1:40, 1:80 (v/v)} for evaluating cell relative growth rate and cell apoptosis/necrosis rate of HPDLFs. The data was analyzed by one-way ANOVA and independent T test. Regardless of light irradiation, cell relative growth rate increased, and the apoptosis/necrosis rate of each resin cement decreased with the increase of gradient dilution. Regardless of gradient dilution, the cell relative growth rate and apoptosis/necrosis rate of RelyX U200 and Maxcem Elite with light irradiation were higher than those without light irradiation. Besides, without light irradiation, Multilink Speed showed higher cell relative growth rate and lower apoptosis/necrosis rate than other cements. Light irradiation and composition difference of self-adhesive resin cements could affect their cytotoxicity on HPDLFs.http://dx.doi.org/10.1155/2018/7823467 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fangfang Sun Ying Liu Yahui Pan Meng Chen Xiangfeng Meng |
spellingShingle |
Fangfang Sun Ying Liu Yahui Pan Meng Chen Xiangfeng Meng Cytotoxicity of Self-Adhesive Resin Cements on Human Periodontal Ligament Fibroblasts BioMed Research International |
author_facet |
Fangfang Sun Ying Liu Yahui Pan Meng Chen Xiangfeng Meng |
author_sort |
Fangfang Sun |
title |
Cytotoxicity of Self-Adhesive Resin Cements on Human Periodontal Ligament Fibroblasts |
title_short |
Cytotoxicity of Self-Adhesive Resin Cements on Human Periodontal Ligament Fibroblasts |
title_full |
Cytotoxicity of Self-Adhesive Resin Cements on Human Periodontal Ligament Fibroblasts |
title_fullStr |
Cytotoxicity of Self-Adhesive Resin Cements on Human Periodontal Ligament Fibroblasts |
title_full_unstemmed |
Cytotoxicity of Self-Adhesive Resin Cements on Human Periodontal Ligament Fibroblasts |
title_sort |
cytotoxicity of self-adhesive resin cements on human periodontal ligament fibroblasts |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2018-01-01 |
description |
The aim of this study was to evaluate the potential cytotoxicity of self-adhesive resin cements with or without light irradiation on human periodontal ligament fibroblasts (HPDLFs) in vitro. Three self-adhesive resin cements (RelyX U200, Maxcem Elite and Multilink Speed) were cured with light or not. Cured cements were stored at 37°C for 24 h in water or Dulbecco’s modified eagle medium (DMEM) medium. Their chromatographic analysis of water-based extract solution was made and then the DMEM-based extract solution was diluted in complete DMEM {1:5, 1:10, 1:20, 1:40, 1:80 (v/v)} for evaluating cell relative growth rate and cell apoptosis/necrosis rate of HPDLFs. The data was analyzed by one-way ANOVA and independent T test. Regardless of light irradiation, cell relative growth rate increased, and the apoptosis/necrosis rate of each resin cement decreased with the increase of gradient dilution. Regardless of gradient dilution, the cell relative growth rate and apoptosis/necrosis rate of RelyX U200 and Maxcem Elite with light irradiation were higher than those without light irradiation. Besides, without light irradiation, Multilink Speed showed higher cell relative growth rate and lower apoptosis/necrosis rate than other cements. Light irradiation and composition difference of self-adhesive resin cements could affect their cytotoxicity on HPDLFs. |
url |
http://dx.doi.org/10.1155/2018/7823467 |
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