Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse
ABSTRACT: Salmonella detection is a key point in food safety testing, because of the frequent association of this pathogen with food poisoning in humans. The standard bacteriological tests currently used for Salmonella-detection are time-consuming; therefore, there is a need to develop alternative m...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Colégio Brasileiro de Patologia Animal (CBPA)
|
Series: | Pesquisa Veterinária Brasileira |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000701300&lng=en&tlng=en |
id |
doaj-47a0add4d788423d94b5fdea827baaf9 |
---|---|
record_format |
Article |
spelling |
doaj-47a0add4d788423d94b5fdea827baaf92020-11-25T01:13:27ZengColégio Brasileiro de Patologia Animal (CBPA)Pesquisa Veterinária Brasileira1678-51503871300130610.1590/1678-5150-pvb-5211S0100-736X2018000701300Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinseIsadora M.O. CorrêaLarissa Q. PereiraIsabella G.O. SilvaRafaela AltarugioBruna D. SmaniottoTarcísio M. SilvaAdriano S. OkamotoRaphael L. Andreatti FilhoABSTRACT: Salmonella detection is a key point in food safety testing, because of the frequent association of this pathogen with food poisoning in humans. The standard bacteriological tests currently used for Salmonella-detection are time-consuming; therefore, there is a need to develop alternative methods to accelerate the detection. In order to accelerate Salmonella diagnosis, we used the immunomagnetic separation assay associated with bacteriophage P22 for the rapid detection of the following Salmonella serovars in chicken rinses of drumsticks, artificially contaminated with 5, 10, and 100 CFU/25mL of bacteria: Salmonella enterica subsp. enterica serovar Heidelberg (S. Heidelberg), Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium). The efficiency of the technique, represented by the time required for detection of positive and negative samples, was compared with that of the standard diagnostic tests used for this pathogen, the bacteriological assay and the polymerase chain reaction (PCR)-based test. This study confirmed the ability of the bacteriophage-associated immunomagnetic separation assay to identify 99.6% of Salmonella-positive samples of the three serovars tested. In contrast, the bacteriological assay and PCR-based test detected 95.1% and 98.5% of the Salmonella-positive samples respectively.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000701300&lng=en&tlng=enDiagnosticSalmonella entericaserovarschicken rinsephage P22Salmonella HeidelbergSalmonella TyphimuriumSalmonella Enteritidisimmunomagnetic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Isadora M.O. Corrêa Larissa Q. Pereira Isabella G.O. Silva Rafaela Altarugio Bruna D. Smaniotto Tarcísio M. Silva Adriano S. Okamoto Raphael L. Andreatti Filho |
spellingShingle |
Isadora M.O. Corrêa Larissa Q. Pereira Isabella G.O. Silva Rafaela Altarugio Bruna D. Smaniotto Tarcísio M. Silva Adriano S. Okamoto Raphael L. Andreatti Filho Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse Pesquisa Veterinária Brasileira Diagnostic Salmonella enterica serovars chicken rinse phage P22 Salmonella Heidelberg Salmonella Typhimurium Salmonella Enteritidis immunomagnetic |
author_facet |
Isadora M.O. Corrêa Larissa Q. Pereira Isabella G.O. Silva Rafaela Altarugio Bruna D. Smaniotto Tarcísio M. Silva Adriano S. Okamoto Raphael L. Andreatti Filho |
author_sort |
Isadora M.O. Corrêa |
title |
Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse |
title_short |
Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse |
title_full |
Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse |
title_fullStr |
Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse |
title_full_unstemmed |
Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse |
title_sort |
comparison of three diagnostic methods for salmonella enterica serovars detection in chicken rinse |
publisher |
Colégio Brasileiro de Patologia Animal (CBPA) |
series |
Pesquisa Veterinária Brasileira |
issn |
1678-5150 |
description |
ABSTRACT: Salmonella detection is a key point in food safety testing, because of the frequent association of this pathogen with food poisoning in humans. The standard bacteriological tests currently used for Salmonella-detection are time-consuming; therefore, there is a need to develop alternative methods to accelerate the detection. In order to accelerate Salmonella diagnosis, we used the immunomagnetic separation assay associated with bacteriophage P22 for the rapid detection of the following Salmonella serovars in chicken rinses of drumsticks, artificially contaminated with 5, 10, and 100 CFU/25mL of bacteria: Salmonella enterica subsp. enterica serovar Heidelberg (S. Heidelberg), Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium). The efficiency of the technique, represented by the time required for detection of positive and negative samples, was compared with that of the standard diagnostic tests used for this pathogen, the bacteriological assay and the polymerase chain reaction (PCR)-based test. This study confirmed the ability of the bacteriophage-associated immunomagnetic separation assay to identify 99.6% of Salmonella-positive samples of the three serovars tested. In contrast, the bacteriological assay and PCR-based test detected 95.1% and 98.5% of the Salmonella-positive samples respectively. |
topic |
Diagnostic Salmonella enterica serovars chicken rinse phage P22 Salmonella Heidelberg Salmonella Typhimurium Salmonella Enteritidis immunomagnetic |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000701300&lng=en&tlng=en |
work_keys_str_mv |
AT isadoramocorrea comparisonofthreediagnosticmethodsforsalmonellaentericaserovarsdetectioninchickenrinse AT larissaqpereira comparisonofthreediagnosticmethodsforsalmonellaentericaserovarsdetectioninchickenrinse AT isabellagosilva comparisonofthreediagnosticmethodsforsalmonellaentericaserovarsdetectioninchickenrinse AT rafaelaaltarugio comparisonofthreediagnosticmethodsforsalmonellaentericaserovarsdetectioninchickenrinse AT brunadsmaniotto comparisonofthreediagnosticmethodsforsalmonellaentericaserovarsdetectioninchickenrinse AT tarcisiomsilva comparisonofthreediagnosticmethodsforsalmonellaentericaserovarsdetectioninchickenrinse AT adrianosokamoto comparisonofthreediagnosticmethodsforsalmonellaentericaserovarsdetectioninchickenrinse AT raphaellandreattifilho comparisonofthreediagnosticmethodsforsalmonellaentericaserovarsdetectioninchickenrinse |
_version_ |
1725162220481937408 |