Association of aldosterone synthase (CYP11B2) gene −344T/C polymorphism with the risk of primary chronic glomerulonephritis in the Polish population

• Main Authors: Magdalena Pawlik, Adrianna Mostowska, Margarita Lianeri, Andrzej Oko, Paweł P Jagodziński
• Format: Article
• Language: English
• Published: Hindawi - SAGE Publishing 2014-12-01
• Series: Journal of the Renin-Angiotensin-Aldosterone System
• Online Access: https://doi.org/10.1177/1470320313489588
• ISSN: 1470-3203; 1752-8976

Introduction: We evaluate whether angiotensinogen AGT M235T (rs699), angiotensin-converting enzyme ACE (I/D) (rs4646994) and aldosterone synthase CYP11B2 –344C/T (rs1799998) polymorphisms can be genetic risk factors of chronic glomerulonephritis (GN) in the Polish population. Materials and methods: The study was conducted in 140 patients with primary chronic GN: mesangial proliferative GN (MesPGN) ( n = 49), IgA nephropathy (IgAN) ( n = 31), membranous nephropathy (MN) ( n = 27), focal segmental glomerulosclerosis (FSGS) ( n = 25), membranoproliferative GN (MPGN) ( n = 4), and minimal change disease (MCD) ( n = 4), and controls ( n = 187). Genotypes were determined by HRM curve analysis for AGT M235T, by PCR and agarose gel separation for ACE (I/D), and by PCR-RFLP for CYP11B2 –344C/T. Results: We found a significant association of the CYP11B2 –344C/T polymorphism in the recessive model with all subtypes of GN (OR = 1.925 (95% CI = 1.152–3.219, p = 0.0118, p corr = 0.0354)). We also observed that the CYP11B2 –344C/T polymorphism in the recessive model may also be an independent significant risk factor of IgAN (OR = 2.743 (95% CI = 1.219–6.172, p = 0.0122, p corr = 0.0366)), FSGS (OR = 2.895 (95% CI = 1.200–6.985, p = 0.0145, p corr = 0.0435)), and all proliferative GNs (MesPGN, IgAN, MPGN) (OR = 2.171 (95% CI = 1.211–3.894, p = 0.0084, p corr = 0.0252)). Conclusion: Our results suggest that the CYP11B2 –344C/T polymorphism might be an independent risk factor of IgAN, FSGS and all proliferative chronic GNs.