COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS
Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of cassava bacterial blight (CBB), a major disease for cassava crops in South America and Africa. Until now the development of the disease is measured via AUDPC (Area Under Disease Progress Curve) but no reliable quantitative methods are...
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2015-05-01
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doaj-47af091e767b4cf68d8f8345b8f210e82021-08-09T23:34:48ZengUniversidad Nacional de ColombiaActa Biológica Colombiana0120-548X1900-16492015-05-0120210.15446/abc.v20n2.4313038165COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTSAlejandra Muñoz Bodnar0Leidy Mariel Cruz Gómez1Adriana Bernal2Boris Szurek3Camilo Ernesto López CarrascalUniversidad Nacional de Colombia.Universidad Pedagógica y Tecnológica de ColombiaLAMFU - Universidad de los AndesIRDXanthomonas axonopodis pv. manihotis (Xam) is the causal agent of cassava bacterial blight (CBB), a major disease for cassava crops in South America and Africa. Until now the development of the disease is measured via AUDPC (Area Under Disease Progress Curve) but no reliable quantitative methods are available probably due to high variability of bacterial growth in planta. To establish an accurate method for bacterial quantification during the course of Xam infection within the host tissues, we analyzed bacterial populations upon stem and leaf-puncturing as well as leaf-clipping of cassava varieties MCOL1522 and SG107-35 challenged with the virulent Xam strain CIO151. Here, we show that the movement of bacteria along the tissues and especially in leaves is stochastic. Moreover, we were able to demonstrate differential growth of virulent Xam strain CIO151 upon stem-puncturing and quantification of bacteria 6 cm. away from the inoculation point of two varieties displaying contrasting levels of susceptibility. RESUMEN Xanthomonas axonopodis pv. manihotis (Xam) es el agente causal del tizón bacteriano de la yuca, una de las principales enfermedades de los cultivos de yuca en América del Sur y África. Hasta ahora, el desarrollo de la enfermedad se mide a través de AUDPC (Area Under Disease Progress curve), pero no hay disponibles métodos cuantitativos fiables, esto debido posiblemente a la alta variabilidad del crecimiento bacteriano en la planta. Para establecer un método exacto para la cuantificación bacteriana durante el curso de la infección Xam dentro de los tejidos del huésped, se analizaron las poblaciones de bacterias sobre tallo y hojas, así como corte de hojas de las variedades de yucaMCOL1522 y SG107-35 con la cepa virulenta CIO151 Xam. En esta investigación se muestra que el movimiento de las bacterias a lo largo de los tejidos y especialmente en las hojas es estocástico. Por otra parte, hemos podido demostrar el crecimiento diferencial de la cepa virulenta Xam CIO151 tras la punción al tallo y la cuantificación de la bacteria a 6 cm de distancia del punto de inoculación de dos variedades que presentan niveles contrastantes de susceptibilidad. https://revistas.unal.edu.co/index.php/actabiol/article/view/43130Bacterial growthcassavainoculation methods. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alejandra Muñoz Bodnar Leidy Mariel Cruz Gómez Adriana Bernal Boris Szurek Camilo Ernesto López Carrascal |
spellingShingle |
Alejandra Muñoz Bodnar Leidy Mariel Cruz Gómez Adriana Bernal Boris Szurek Camilo Ernesto López Carrascal COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS Acta Biológica Colombiana Bacterial growth cassava inoculation methods. |
author_facet |
Alejandra Muñoz Bodnar Leidy Mariel Cruz Gómez Adriana Bernal Boris Szurek Camilo Ernesto López Carrascal |
author_sort |
Alejandra Muñoz Bodnar |
title |
COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS |
title_short |
COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS |
title_full |
COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS |
title_fullStr |
COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS |
title_full_unstemmed |
COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS |
title_sort |
comparing inoculation methods to evaluate the growth of xanthomonas axonopodis pv. manihotis on cassava plants |
publisher |
Universidad Nacional de Colombia |
series |
Acta Biológica Colombiana |
issn |
0120-548X 1900-1649 |
publishDate |
2015-05-01 |
description |
Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of cassava bacterial blight (CBB), a major disease for cassava crops in South America and Africa. Until now the development of the disease is measured via AUDPC (Area Under Disease Progress Curve) but no reliable quantitative methods are available probably due to high variability of bacterial growth in planta. To establish an accurate method for bacterial quantification during the course of Xam infection within the host tissues, we analyzed bacterial populations upon stem and leaf-puncturing as well as leaf-clipping of cassava varieties MCOL1522 and SG107-35 challenged with the virulent Xam strain CIO151. Here, we show that the movement of bacteria along the tissues and especially in leaves is stochastic. Moreover, we were able to demonstrate differential growth of virulent Xam strain CIO151 upon stem-puncturing and quantification of bacteria 6 cm. away from the inoculation point of two varieties displaying contrasting levels of susceptibility.
RESUMEN
Xanthomonas axonopodis pv. manihotis (Xam) es el agente causal del tizón bacteriano de la yuca, una de las principales enfermedades de los cultivos de yuca en América del Sur y África. Hasta ahora, el desarrollo de la enfermedad se mide a través de AUDPC (Area Under Disease Progress curve), pero no hay disponibles métodos cuantitativos fiables, esto debido posiblemente a la alta variabilidad del crecimiento bacteriano en la planta. Para establecer un método exacto para la cuantificación bacteriana durante el curso de la infección Xam dentro de los tejidos del huésped, se analizaron las poblaciones de bacterias sobre tallo y hojas, así como corte de hojas de las variedades de yucaMCOL1522 y SG107-35 con la cepa virulenta CIO151 Xam. En esta investigación se muestra que el movimiento de las bacterias a lo largo de los tejidos y especialmente en las hojas es estocástico. Por otra parte, hemos podido demostrar el crecimiento diferencial de la cepa virulenta Xam CIO151 tras la punción al tallo y la cuantificación de la bacteria a 6 cm de distancia del punto de inoculación de dos variedades que presentan niveles contrastantes de susceptibilidad.
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topic |
Bacterial growth cassava inoculation methods. |
url |
https://revistas.unal.edu.co/index.php/actabiol/article/view/43130 |
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