Clinical Outcomes of Single Mosaic Embryo Transfer: High-Level or Low-Level Mosaic Embryo, Does it Matter?
Recently, reports showed that embryos identified as mosaic after preimplantation genetic testing for aneuploid (PGT-A) could result in live birth with lower pregnancy and higher pregnancy loss rates compared with euploid embryos. However, the effects of mosaicism level on reproductive outcomes remai...
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doaj-47b173d3594548fe890c06f5c80aac4e2020-11-25T03:23:05ZengMDPI AGJournal of Clinical Medicine2077-03832020-06-0191695169510.3390/jcm9061695Clinical Outcomes of Single Mosaic Embryo Transfer: High-Level or Low-Level Mosaic Embryo, Does it Matter?Pin-Yao Lin0Chun-I Lee1En-Hui Cheng2Chun-Chia Huang3Tsung-Hsien Lee4Hui-Hsin Shih5Yi-Ping Pai6Yi-Chun Chen7Maw-Sheng Lee8Institute of Medicine, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., South District, Taichung City 40201, TaiwanInstitute of Medicine, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., South District, Taichung City 40201, TaiwanDivision of Infertility, Lee Women’s Hospital, No. 30-6, Sec. 1, Changping Road, Beitun District, Taichung City 406, TaiwanDivision of Infertility, Lee Women’s Hospital, No. 30-6, Sec. 1, Changping Road, Beitun District, Taichung City 406, TaiwanInstitute of Medicine, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., South District, Taichung City 40201, TaiwanDivision of Infertility, Lee Women’s Hospital, No. 30-6, Sec. 1, Changping Road, Beitun District, Taichung City 406, TaiwanDivision of Infertility, Lee Women’s Hospital, No. 30-6, Sec. 1, Changping Road, Beitun District, Taichung City 406, TaiwanDivision of Infertility, Lee Women’s Hospital, No. 30-6, Sec. 1, Changping Road, Beitun District, Taichung City 406, TaiwanInstitute of Medicine, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., South District, Taichung City 40201, TaiwanRecently, reports showed that embryos identified as mosaic after preimplantation genetic testing for aneuploid (PGT-A) could result in live birth with lower pregnancy and higher pregnancy loss rates compared with euploid embryos. However, the effects of mosaicism level on reproductive outcomes remain controversial. This study aimed to examine the level of mosaicism on pregnancy outcomes. Single mosaic embryo transfer was offered to 108 women who only had mosaic embryos. Mosaic embryos were labeled by utilizing next generation sequencing (NGS) based PGT-A for day 5/6 trophectoderm (TE)biopsies. TE biopsies containing < 50% abnormal cells were classified as low-level mosaicism and ≥ 50% as high-level mosaicism. To further confirm the concordance of chromosome constitution between TE and inner cell mass (ICM), 41 remaining embryos designated as mosaic blastocysts donated for research were also analyzed. Comparable live birth rate (LBR) but higher miscarriage rate (MR) was found in the high-level group. (LBR: low vs. high: 44.5% vs. 36%; <i>p </i>= 0.45, MR: low vs. high: 5.1% vs. 30.7%; <i>p </i>= 0.012). Analyses of TE and ICM from the remaining mosaic blastocysts show a poor concordance. This preliminary study demonstrated that high-level mosaic embryos could result in comparable LBR but higher MR.https://www.mdpi.com/2077-0383/9/6/1695in vitro fertilizationpreimplantation genetic testing for aneuploidyaneuploidymosaic embryonext-generation sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pin-Yao Lin Chun-I Lee En-Hui Cheng Chun-Chia Huang Tsung-Hsien Lee Hui-Hsin Shih Yi-Ping Pai Yi-Chun Chen Maw-Sheng Lee |
spellingShingle |
Pin-Yao Lin Chun-I Lee En-Hui Cheng Chun-Chia Huang Tsung-Hsien Lee Hui-Hsin Shih Yi-Ping Pai Yi-Chun Chen Maw-Sheng Lee Clinical Outcomes of Single Mosaic Embryo Transfer: High-Level or Low-Level Mosaic Embryo, Does it Matter? Journal of Clinical Medicine in vitro fertilization preimplantation genetic testing for aneuploidy aneuploidy mosaic embryo next-generation sequencing |
author_facet |
Pin-Yao Lin Chun-I Lee En-Hui Cheng Chun-Chia Huang Tsung-Hsien Lee Hui-Hsin Shih Yi-Ping Pai Yi-Chun Chen Maw-Sheng Lee |
author_sort |
Pin-Yao Lin |
title |
Clinical Outcomes of Single Mosaic Embryo Transfer: High-Level or Low-Level Mosaic Embryo, Does it Matter? |
title_short |
Clinical Outcomes of Single Mosaic Embryo Transfer: High-Level or Low-Level Mosaic Embryo, Does it Matter? |
title_full |
Clinical Outcomes of Single Mosaic Embryo Transfer: High-Level or Low-Level Mosaic Embryo, Does it Matter? |
title_fullStr |
Clinical Outcomes of Single Mosaic Embryo Transfer: High-Level or Low-Level Mosaic Embryo, Does it Matter? |
title_full_unstemmed |
Clinical Outcomes of Single Mosaic Embryo Transfer: High-Level or Low-Level Mosaic Embryo, Does it Matter? |
title_sort |
clinical outcomes of single mosaic embryo transfer: high-level or low-level mosaic embryo, does it matter? |
publisher |
MDPI AG |
series |
Journal of Clinical Medicine |
issn |
2077-0383 |
publishDate |
2020-06-01 |
description |
Recently, reports showed that embryos identified as mosaic after preimplantation genetic testing for aneuploid (PGT-A) could result in live birth with lower pregnancy and higher pregnancy loss rates compared with euploid embryos. However, the effects of mosaicism level on reproductive outcomes remain controversial. This study aimed to examine the level of mosaicism on pregnancy outcomes. Single mosaic embryo transfer was offered to 108 women who only had mosaic embryos. Mosaic embryos were labeled by utilizing next generation sequencing (NGS) based PGT-A for day 5/6 trophectoderm (TE)biopsies. TE biopsies containing < 50% abnormal cells were classified as low-level mosaicism and ≥ 50% as high-level mosaicism. To further confirm the concordance of chromosome constitution between TE and inner cell mass (ICM), 41 remaining embryos designated as mosaic blastocysts donated for research were also analyzed. Comparable live birth rate (LBR) but higher miscarriage rate (MR) was found in the high-level group. (LBR: low vs. high: 44.5% vs. 36%; <i>p </i>= 0.45, MR: low vs. high: 5.1% vs. 30.7%; <i>p </i>= 0.012). Analyses of TE and ICM from the remaining mosaic blastocysts show a poor concordance. This preliminary study demonstrated that high-level mosaic embryos could result in comparable LBR but higher MR. |
topic |
in vitro fertilization preimplantation genetic testing for aneuploidy aneuploidy mosaic embryo next-generation sequencing |
url |
https://www.mdpi.com/2077-0383/9/6/1695 |
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