Coeliac Disease in Elderly Patients: Value of Coeliac Lymphogram for Diagnosis
(1) Background: Although a meta-analysis reported that the sensitivity of CD3+ TCRγδ+ cells for coeliac disease diagnosis was >93%, a recent study has suggested that sensitivity decreased to 65% in elderly patients. (2) Aim: To evaluate whether the sensitivity of intraepithelial lymphocyte cytome...
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doaj-47bb3a3c24dd443dbc60fbfc41c9881a2021-09-26T00:51:11ZengMDPI AGNutrients2072-66432021-08-01132984298410.3390/nu13092984Coeliac Disease in Elderly Patients: Value of Coeliac Lymphogram for DiagnosisFernando Fernández-Bañares0Sergio Farrais1Montserrat Planella2Josefa Melero3Natalia López-Palacios4Santiago Vivas5Luis Fernández-Salazar6Ana Pilar Lanzarote7Pablo Ruiz-Ramírez8Marta Aguilar-Criado9Judith Vidal10Aureli Esquerda11Cristina Serrano12Concepción Núñez13Department of Gastroenterology, Hospital Universitari Mutua Terrassa, 08221 Terrassa, SpainDepartment of Gastroenterology, Hospital Fundación Jiménez Díaz, 28040 Madrid, SpainDepartment of Gastroenterology, Hospital Universitari Arnau de Vilanova, 25198 Lleida, SpainDepartment Immunology and Genetics, Hospital Universitario de Badajoz, 06080 Badajoz, SpainDepartment of Gastroenterology, Hospital Clínico San Carlos, IdISSC, 28040 Madrid, SpainDepartment of Gastroenterology, Complejo Asistencial Universitario de León, 24071 León, SpainDepartment of Gastroenterology, Hospital Clínico Universitario de Valladolid, Gerencia Regional de Salud (SACYL), Facultad de Medicina, Universidad de Valladolid, 47002 Valladolid, SpainDepartment of Gastroenterology, Hospital Fundación Jiménez Díaz, 28040 Madrid, SpainDepartment of Gastroenterology, Hospital Universitari Mutua Terrassa, 08221 Terrassa, SpainDepartment Immunology and Genetics, Hospital Universitario de Badajoz, 06080 Badajoz, SpainDepartment of Flow Cytometry, CATLAB, 08232 Viladecavalls, SpainDepartment of Clinical Laboratory, Hospital Universitari Arnau de Vilanova, 25198 Lleida, SpainDepartment of Clinical Immunology, Hospital Fundación Jiménez Díaz, 28040 Madrid, SpainLaboratorio de Investigación en Genética de Enfermedades Complejas, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria Hospital Clínico San Carlos (IdISSC), 28040 Madrid, Spain(1) Background: Although a meta-analysis reported that the sensitivity of CD3+ TCRγδ+ cells for coeliac disease diagnosis was >93%, a recent study has suggested that sensitivity decreased to 65% in elderly patients. (2) Aim: To evaluate whether the sensitivity of intraepithelial lymphocyte cytometric patterns for coeliac disease diagnosis changes with advanced age. (3) Methods: We performed a multicentre study including 127 coeliac disease patients ≥ 50 years: 87 with baseline cytometry (45 aged 50–59 years; 23 aged 60–69 years; 19 aged ≥ 70 years), 16 also with a follow-up cytometry (on a gluten-free diet); and 40 with only follow-up cytometry. (4) Results: In Marsh 3 patients, a sensitivity of 94.7%, 88.9% and 86.7% was observed for each age group using a cut-off value of TCRγδ+ >10% (<i>p</i> = 0.27); and a sensitivity of 84.2%, 83.4% and 53.3% for a cut-off value >14% (<i>p</i> = 0.02; 50–69 vs. ≥70 years), with difference between applying a cut-off of 10% or 14% (<i>p</i> = 0.008). The TCRγδ+ count in the ≥70 years group was lower than in the other groups (<i>p</i> = 0.014). (5) Conclusion: In coeliac patients ≥ 70 years, the TCRγδ+ count decreases and the cut-off point of >10% is more accurate than >14%.https://www.mdpi.com/2072-6643/13/9/2984coeliacTCRγδ+CD3−flow cytometryelderly |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fernando Fernández-Bañares Sergio Farrais Montserrat Planella Josefa Melero Natalia López-Palacios Santiago Vivas Luis Fernández-Salazar Ana Pilar Lanzarote Pablo Ruiz-Ramírez Marta Aguilar-Criado Judith Vidal Aureli Esquerda Cristina Serrano Concepción Núñez |
spellingShingle |
Fernando Fernández-Bañares Sergio Farrais Montserrat Planella Josefa Melero Natalia López-Palacios Santiago Vivas Luis Fernández-Salazar Ana Pilar Lanzarote Pablo Ruiz-Ramírez Marta Aguilar-Criado Judith Vidal Aureli Esquerda Cristina Serrano Concepción Núñez Coeliac Disease in Elderly Patients: Value of Coeliac Lymphogram for Diagnosis Nutrients coeliac TCRγδ+ CD3− flow cytometry elderly |
author_facet |
Fernando Fernández-Bañares Sergio Farrais Montserrat Planella Josefa Melero Natalia López-Palacios Santiago Vivas Luis Fernández-Salazar Ana Pilar Lanzarote Pablo Ruiz-Ramírez Marta Aguilar-Criado Judith Vidal Aureli Esquerda Cristina Serrano Concepción Núñez |
author_sort |
Fernando Fernández-Bañares |
title |
Coeliac Disease in Elderly Patients: Value of Coeliac Lymphogram for Diagnosis |
title_short |
Coeliac Disease in Elderly Patients: Value of Coeliac Lymphogram for Diagnosis |
title_full |
Coeliac Disease in Elderly Patients: Value of Coeliac Lymphogram for Diagnosis |
title_fullStr |
Coeliac Disease in Elderly Patients: Value of Coeliac Lymphogram for Diagnosis |
title_full_unstemmed |
Coeliac Disease in Elderly Patients: Value of Coeliac Lymphogram for Diagnosis |
title_sort |
coeliac disease in elderly patients: value of coeliac lymphogram for diagnosis |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2021-08-01 |
description |
(1) Background: Although a meta-analysis reported that the sensitivity of CD3+ TCRγδ+ cells for coeliac disease diagnosis was >93%, a recent study has suggested that sensitivity decreased to 65% in elderly patients. (2) Aim: To evaluate whether the sensitivity of intraepithelial lymphocyte cytometric patterns for coeliac disease diagnosis changes with advanced age. (3) Methods: We performed a multicentre study including 127 coeliac disease patients ≥ 50 years: 87 with baseline cytometry (45 aged 50–59 years; 23 aged 60–69 years; 19 aged ≥ 70 years), 16 also with a follow-up cytometry (on a gluten-free diet); and 40 with only follow-up cytometry. (4) Results: In Marsh 3 patients, a sensitivity of 94.7%, 88.9% and 86.7% was observed for each age group using a cut-off value of TCRγδ+ >10% (<i>p</i> = 0.27); and a sensitivity of 84.2%, 83.4% and 53.3% for a cut-off value >14% (<i>p</i> = 0.02; 50–69 vs. ≥70 years), with difference between applying a cut-off of 10% or 14% (<i>p</i> = 0.008). The TCRγδ+ count in the ≥70 years group was lower than in the other groups (<i>p</i> = 0.014). (5) Conclusion: In coeliac patients ≥ 70 years, the TCRγδ+ count decreases and the cut-off point of >10% is more accurate than >14%. |
topic |
coeliac TCRγδ+ CD3− flow cytometry elderly |
url |
https://www.mdpi.com/2072-6643/13/9/2984 |
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