Anti-dsDNA, anti-nucleosome and anti-C1q antibodies as disease activity markers in patients with systemic lupus erythematosus
Introduction. In spite of the growing number of reports on the study of anti-nucleosome and anti-C1q antibodies, there are still controversies on their significance as disease activity markers in patients with systemic lupus erythematosus (SLE) and their use in everyday clinical practice....
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Online Access: | http://www.doiserbia.nb.rs/img/doi/0370-8179/2014/0370-81791408431Z.pdf |
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doaj-47bf5181bc3c46edb07020938cd496c12021-01-02T00:39:38ZengSerbian Medical SocietySrpski Arhiv za Celokupno Lekarstvo0370-81792014-01-011427-843143610.2298/SARH1408431Z0370-81791408431ZAnti-dsDNA, anti-nucleosome and anti-C1q antibodies as disease activity markers in patients with systemic lupus erythematosusŽivković Valentina0Stanković Aleksandra1Cvetković Tatjana2Mitić Branka3Kostić Svetislav4Nedović Jovan5Stamenković Bojana6Institute for Treatment and Rehabilitation “Niška Banja”, NišInstitute for Treatment and Rehabilitation “Niška Banja”, NišClinic for Nephrology, Clinical Center, NišClinic for Nephrology, Clinical Center, NišClinic for Nephrology, Clinical Center, NišInstitute for Treatment and Rehabilitation “Niška Banja”, NišInstitute for Treatment and Rehabilitation “Niška Banja”, NišIntroduction. In spite of the growing number of reports on the study of anti-nucleosome and anti-C1q antibodies, there are still controversies on their significance as disease activity markers in patients with systemic lupus erythematosus (SLE) and their use in everyday clinical practice. Objective. Our aim was to assess the presence of anti-dsDNA, anti-nucleosome and anti-C1q antibodies in SLE patients, as well as to establish their sensitivity, specificity, positive and negative predictive value, and their correlation with SLE and lupus nephritis clinical activity. Methods. The study enrolled 85 patients aged 45.3±9.7 years on the average, with SLE of average duration 10.37±7.99 years, hospitalized at the Institute „Niška Banja“ during 2011, and 30 healthy individuals as controls. Disease activity was assessed using Systemic Lupus Erythematosus Disease Activity Index (SLEDAI). In all examinees the levels of anti-dsDNA, anti-nucleosome and anti-C1q antibodies were measured using the ELISA method with Alegria Test Strips Orgentec (Germany). Results. Patients with active lupus nephritis had a higher presence of anti-C1q antibodies and higher co-positivity of anti-dsDNA, anti-nucleosome, and anti-C1q antibodies compared to those with inactive lupus nephritis (77.77% vs. 21.74%; p<0.01). SLE patients with SLEDAI ≥11 had a higher presence of antinucleosome (93.75% vs. 64.15%; p<0.01) and anti-C1q antibodies (46.87% vs. 22.64%; p<0.05), as well as a higher mean level of anti-nucleosome antibodies (107.79±83.46 U/ml vs. 57.81±63.15 U/ml; p<0.05), compared to those with SLEDAI of 0-10. There was a positive correlation between the SLEDAI and the level of anti-dsDNA (r=0.290; p<0.01), anti-nucleosome (r=0.443; p<0.001), and anti-C1q antibodies (r=0.382; p<0.001). Only anti-C1q antibodies demonstrated correlation with proteinuria (r=0.445; p<0.001). Conclusion. Anti-nucleosome and anti-C1q antibodies demonstrated association with SLE and lupus nephritis activity, suggesting their potential usefulness in making predictions about lupus nephritis and assessment of disease activity.http://www.doiserbia.nb.rs/img/doi/0370-8179/2014/0370-81791408431Z.pdfsystemic lupus erythematosusanti-dsDNA antibodiesanti-nucleosome antibodiesanti-C1qantibodieslupus nephritisSLEDAI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Živković Valentina Stanković Aleksandra Cvetković Tatjana Mitić Branka Kostić Svetislav Nedović Jovan Stamenković Bojana |
spellingShingle |
Živković Valentina Stanković Aleksandra Cvetković Tatjana Mitić Branka Kostić Svetislav Nedović Jovan Stamenković Bojana Anti-dsDNA, anti-nucleosome and anti-C1q antibodies as disease activity markers in patients with systemic lupus erythematosus Srpski Arhiv za Celokupno Lekarstvo systemic lupus erythematosus anti-dsDNA antibodies anti-nucleosome antibodies anti-C1qantibodies lupus nephritis SLEDAI |
author_facet |
Živković Valentina Stanković Aleksandra Cvetković Tatjana Mitić Branka Kostić Svetislav Nedović Jovan Stamenković Bojana |
author_sort |
Živković Valentina |
title |
Anti-dsDNA, anti-nucleosome and anti-C1q antibodies as disease activity markers in patients with systemic lupus erythematosus |
title_short |
Anti-dsDNA, anti-nucleosome and anti-C1q antibodies as disease activity markers in patients with systemic lupus erythematosus |
title_full |
Anti-dsDNA, anti-nucleosome and anti-C1q antibodies as disease activity markers in patients with systemic lupus erythematosus |
title_fullStr |
Anti-dsDNA, anti-nucleosome and anti-C1q antibodies as disease activity markers in patients with systemic lupus erythematosus |
title_full_unstemmed |
Anti-dsDNA, anti-nucleosome and anti-C1q antibodies as disease activity markers in patients with systemic lupus erythematosus |
title_sort |
anti-dsdna, anti-nucleosome and anti-c1q antibodies as disease activity markers in patients with systemic lupus erythematosus |
publisher |
Serbian Medical Society |
series |
Srpski Arhiv za Celokupno Lekarstvo |
issn |
0370-8179 |
publishDate |
2014-01-01 |
description |
Introduction. In spite of the growing number of reports on the study of
anti-nucleosome and anti-C1q antibodies, there are still controversies on
their significance as disease activity markers in patients with systemic
lupus erythematosus (SLE) and their use in everyday clinical practice.
Objective. Our aim was to assess the presence of anti-dsDNA, anti-nucleosome
and anti-C1q antibodies in SLE patients, as well as to establish their
sensitivity, specificity, positive and negative predictive value, and their
correlation with SLE and lupus nephritis clinical activity. Methods. The
study enrolled 85 patients aged 45.3±9.7 years on the average, with SLE of
average duration 10.37±7.99 years, hospitalized at the Institute „Niška
Banja“ during 2011, and 30 healthy individuals as controls. Disease activity
was assessed using Systemic Lupus Erythematosus Disease Activity Index
(SLEDAI). In all examinees the levels of anti-dsDNA, anti-nucleosome and
anti-C1q antibodies were measured using the ELISA method with Alegria Test
Strips Orgentec (Germany). Results. Patients with active lupus nephritis had
a higher presence of anti-C1q antibodies and higher co-positivity of
anti-dsDNA, anti-nucleosome, and anti-C1q antibodies compared to those with
inactive lupus nephritis (77.77% vs. 21.74%; p<0.01). SLE patients with
SLEDAI ≥11 had a higher presence of antinucleosome (93.75% vs. 64.15%;
p<0.01) and anti-C1q antibodies (46.87% vs. 22.64%; p<0.05), as well as a
higher mean level of anti-nucleosome antibodies (107.79±83.46 U/ml vs.
57.81±63.15 U/ml; p<0.05), compared to those with SLEDAI of 0-10. There was a
positive correlation between the SLEDAI and the level of anti-dsDNA (r=0.290;
p<0.01), anti-nucleosome (r=0.443; p<0.001), and anti-C1q antibodies
(r=0.382; p<0.001). Only anti-C1q antibodies demonstrated correlation with
proteinuria (r=0.445; p<0.001). Conclusion. Anti-nucleosome and anti-C1q
antibodies demonstrated association with SLE and lupus nephritis activity,
suggesting their potential usefulness in making predictions about lupus
nephritis and assessment of disease activity. |
topic |
systemic lupus erythematosus anti-dsDNA antibodies anti-nucleosome antibodies anti-C1qantibodies lupus nephritis SLEDAI |
url |
http://www.doiserbia.nb.rs/img/doi/0370-8179/2014/0370-81791408431Z.pdf |
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