Penambahan xylitol dalam glukosa, sukrosa terhadap pertumbuhan Streptococcus mutans (in vitro) (The Additional xylitol in glucose and sucrose on growth of Mutans Streptococci (in vitro))

• Main Authors: Susilowati Susilowati, Udijanto Tedjosasongko, FX Suhariadji
• Format: Article
• Language: English
• Published: Universitas Airlangga 2014-12-01
• Series: Dental Journal: Majalah Kedokteran Gigi
• Subjects: Xylitol; glucose; sucrose; Streptococcus mutans; dental caries; glukosa; sukrosa; karies gigi
• Online Access: http://e-journal.unair.ac.id/index.php/MKG/article/view/710
• ISSN: 1978-3728; 2442-9740

<p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background:</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em> Xylitol is a sugar alcohol group consisting of five-carbon chain and the sugar substitutes are recommended to prevent </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>caries. Dietary sugars known as a good substrate for the growth of Streptococcus mutans (S. mutans). Two types of sugar, xylitol and </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dietary sugars have different effects on the growth of S.mutans. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The objective of this study were to determine the minimal </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>inhibitory concentration (MIC) of xylitol on the growth of S.mutans and to determine the addition of xylitol in glucose and sucrose in </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>the growth of S. mutans in vitro. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The samples were divided into 3 groups: xylitol group, xylitol and sucrose combination </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>group, and xylitol and glucose combination group . In all groups were tested against S.mutans growth in various concentrations. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Results: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The minimum inhibitory concentration against S.mutans xylitol was equal to 0.625%. The addition of xylitol in sucrose the </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>inhibition of S.mutans growth occurred at concentrations of 0.625 % and 2.5%. The addition of xylitol in glucose inhibited the growth </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of S.mutans at all concentrations. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>This study showed that the combination of xylitol with dietary sugars could inhibit the </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>growth of S.mutans.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p><p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Latar belakang: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Xylitol adalah golongan gula alkohol yang terdiri dari lima rantai karbon dan merupakan sugar substitutes yang </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dianjurkan untuk mencegah terjadinya karies. Dietary sugars diketahui sebagai substrat yang baik untuk pertumbuhan bakteri rongga </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>mulut salah satunya Streptococcus mutans (S. mutans). Dua jenis gula yaitu xylitol dan dietary sugars memiliki pengaruh yang berbeda </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>pada pertumbuhan S. mutans. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Tujuan: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Tujuan dari penelitian ini adalah meneliti konsentrasi hambat minimal (Minimal Inhibitory </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Concentration/ MIC) xylitol terhadap pertumbuhan S mutans dan meneliti pengaruh penambahan xylitol dalam glukosa dan dalam </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>sukrosa terhadap pertumbuhan S. mutans secara in vitro. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Metode: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Sampel dibagi dalam 3 kelompok: kelompok xylitol, kelompok </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>kombinasi xylitol dan sukrosa, dan kelompok kombinasi xylitol dan glukosa. Pada ketiga kelompok tersebut dilakukan pengujian </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>terhadap pertumbuhan S.mutans dalam berbagai konsentrasi. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Hasil: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Konsentrasi hambat minimum xylitol terhadap S. mutans adalah </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>sebesar 0,625%. Pada penambahan xylitol dalam sukrosa terjadi penghambatan S. mutans pada konsentrasi 0,625% dan 2,5%. Pada </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>penambahan xylitol dalam glukosa terjadi penghambatan S. mutans pada semua konsentrasi. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Simpulan: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Penelitian ini menunjukkan </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>bahwa kombinasi xylitol dengan glukosa dan dengan sukrosa dapat menghambat pertumbuhan S.mutans.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span></span></span></span></span></span></span></span></p>