Improvement of α-amylase to the metabolism adaptions of soil bacteria against PFOS exposure
Toxicity of perfluoroalkyl substances (PFASs) in soils towards bacteria shows an impact on its ecosystem function. This study aims to obtain insight into the effect of hydrolase (e.g. α-amylase) in soil on metabolism adaptions of bacteria (e.g. Bacillus substilis) against PFOS exposure. Results show...
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doaj-47dc3e8c076b45b89c0ae97f866925562021-04-23T06:15:20ZengElsevierEcotoxicology and Environmental Safety0147-65132021-01-01208111770Improvement of α-amylase to the metabolism adaptions of soil bacteria against PFOS exposureTongtong Zheng0Jie Li1Chunguang Liu2Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, China−America CRC for Environment & Health of Shandong Province, School of Environmental Science and Engineering, Shandong University, 72# Jimo Binhai Road, Qingdao, Shandong 266237, PR ChinaShandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, China−America CRC for Environment & Health of Shandong Province, School of Environmental Science and Engineering, Shandong University, 72# Jimo Binhai Road, Qingdao, Shandong 266237, PR ChinaShandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, China−America CRC for Environment & Health of Shandong Province, School of Environmental Science and Engineering, Shandong University, 72# Jimo Binhai Road, Qingdao, Shandong 266237, PR China; Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, PR China; Corresponding author at: Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, China−America CRC for Environment & Health of Shandong Province, School of Environmental Science and Engineering, Shandong University, 72# Jimo Binhai Road, Qingdao, Shandong 266237, PR China.Toxicity of perfluoroalkyl substances (PFASs) in soils towards bacteria shows an impact on its ecosystem function. This study aims to obtain insight into the effect of hydrolase (e.g. α-amylase) in soil on metabolism adaptions of bacteria (e.g. Bacillus substilis) against PFOS exposure. Results show that exogenous α-amylase alleviates PFOS toxicity to bacteria growth, disturbance to membrane permeability and stimulation to reactive oxygen species (ROS) production. The mechanisms were owing to that α-amylase strongly influences the strategies of metabolism adaptions of bacteria against PFOS stress. In details, α-amylase prompts bacteria to regulate the secretion of extracellular polymeric substances (EPSs) and the production of metabolic signal (acetic acid), which leads to changes in the physicochemical properties (hydrophilicity, surface charge) of the bacterial surface and the inactivation of the interaction with PFOS, thereby reducing the PFOS toxicity. Molecular simulations show that PFOS combines with Srt A at Gly 53 and Trp 171, which may induce the increase of permeability and changes of surface characteristics. Meanwhile, α-amylase competes with Srt A to bind PFOS at Arg 125 and Lys 176. This competition changes the physicochemical characteristics of PFOS and its bioavailability, further improving the metabolism adaptions of bacteria against PFOS. Altogether, this work provides direct evidences about α-amylase buffering effect of PFOS and demonstrates that the presence of α-amylase affects the essential but complex metabolic response in bacteria triggered by PFOS.http://www.sciencedirect.com/science/article/pii/S0147651320316067α-amylasePFOSSoil bacteriaBioavailabilityMetabolism adaption |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tongtong Zheng Jie Li Chunguang Liu |
spellingShingle |
Tongtong Zheng Jie Li Chunguang Liu Improvement of α-amylase to the metabolism adaptions of soil bacteria against PFOS exposure Ecotoxicology and Environmental Safety α-amylase PFOS Soil bacteria Bioavailability Metabolism adaption |
author_facet |
Tongtong Zheng Jie Li Chunguang Liu |
author_sort |
Tongtong Zheng |
title |
Improvement of α-amylase to the metabolism adaptions of soil bacteria against PFOS exposure |
title_short |
Improvement of α-amylase to the metabolism adaptions of soil bacteria against PFOS exposure |
title_full |
Improvement of α-amylase to the metabolism adaptions of soil bacteria against PFOS exposure |
title_fullStr |
Improvement of α-amylase to the metabolism adaptions of soil bacteria against PFOS exposure |
title_full_unstemmed |
Improvement of α-amylase to the metabolism adaptions of soil bacteria against PFOS exposure |
title_sort |
improvement of α-amylase to the metabolism adaptions of soil bacteria against pfos exposure |
publisher |
Elsevier |
series |
Ecotoxicology and Environmental Safety |
issn |
0147-6513 |
publishDate |
2021-01-01 |
description |
Toxicity of perfluoroalkyl substances (PFASs) in soils towards bacteria shows an impact on its ecosystem function. This study aims to obtain insight into the effect of hydrolase (e.g. α-amylase) in soil on metabolism adaptions of bacteria (e.g. Bacillus substilis) against PFOS exposure. Results show that exogenous α-amylase alleviates PFOS toxicity to bacteria growth, disturbance to membrane permeability and stimulation to reactive oxygen species (ROS) production. The mechanisms were owing to that α-amylase strongly influences the strategies of metabolism adaptions of bacteria against PFOS stress. In details, α-amylase prompts bacteria to regulate the secretion of extracellular polymeric substances (EPSs) and the production of metabolic signal (acetic acid), which leads to changes in the physicochemical properties (hydrophilicity, surface charge) of the bacterial surface and the inactivation of the interaction with PFOS, thereby reducing the PFOS toxicity. Molecular simulations show that PFOS combines with Srt A at Gly 53 and Trp 171, which may induce the increase of permeability and changes of surface characteristics. Meanwhile, α-amylase competes with Srt A to bind PFOS at Arg 125 and Lys 176. This competition changes the physicochemical characteristics of PFOS and its bioavailability, further improving the metabolism adaptions of bacteria against PFOS. Altogether, this work provides direct evidences about α-amylase buffering effect of PFOS and demonstrates that the presence of α-amylase affects the essential but complex metabolic response in bacteria triggered by PFOS. |
topic |
α-amylase PFOS Soil bacteria Bioavailability Metabolism adaption |
url |
http://www.sciencedirect.com/science/article/pii/S0147651320316067 |
work_keys_str_mv |
AT tongtongzheng improvementofaamylasetothemetabolismadaptionsofsoilbacteriaagainstpfosexposure AT jieli improvementofaamylasetothemetabolismadaptionsofsoilbacteriaagainstpfosexposure AT chunguangliu improvementofaamylasetothemetabolismadaptionsofsoilbacteriaagainstpfosexposure |
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1721513074966396928 |