Determination of total L-Ascorbic Acid by high performance liquid chromatography in human plasma

• Main Authors: Oveisi MR, Sadeghi N
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2001-07-01
• Series: Acta Medica Iranica
• Subjects: L-Ascorbic acid; Total vitamin C
• Online Access: http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/1149.pdf&manuscript_id=1149

The total vitamin C content in human plasma is widely accepted as an indicator of the tissue status of vitamin C. A liquid chromatography method with ultraviolet detector (264 nm) for measuring ascorbic acid in human plasma was developed. A C18 reversed-phase column and cetrimide as an ion-pairing agent was employed. Ascorbic acid (AA) was measured after reducing L-dehydroascorbic acid to L-ascorbic acid with dithiothreitol. The stability of the ascorbic acid in plasma, metaphosphoric acid and trichloroacetic acid was also evaluated. The analytical parameters, including linearity (1-60 µg/ml), accuracy (98.98%), repeatability (2.8%) and reproducibility (7.2%), showed that the method is reliable for measuring the total vitamin C content in plasma.