Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver disease
Background/Aims Intestinal cholesterol absorption includes intestinal Niemann-Pick C1-like 1 (NPC1L1) and is an important target pathway in nonalcoholic fatty liver disease (NAFLD). We investigated the expression of NPC1L1 and its correlation with liver X receptor (LXR) expression in peripheral mono...
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doaj-481585e45c044ca59ce2b78fa5fd76622021-08-10T01:10:38ZengThe Korean Association of Internal MedicineThe Korean Journal of Internal Medicine1226-33032005-66482019-07-0134477778410.3904/kjim.2017.100170027Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver diseaseSang Bong Ahn0Dae Won Jun1Kiseok Jang2Byung Hoon Lee3Kye Jung Shin4 Department of Internal Medicine, Nowon Eulji Medical Center, Eulji University School of Medicine, Seoul, Korea Department of Internal Medicine, Hanyang University School of Medicine, Seoul, Korea Department of Pathology, Hanyang University School of Medicine, Seoul, Korea College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea College of Pharmacy, The Catholic University of Korea, Bucheon, KoreaBackground/Aims Intestinal cholesterol absorption includes intestinal Niemann-Pick C1-like 1 (NPC1L1) and is an important target pathway in nonalcoholic fatty liver disease (NAFLD). We investigated the expression of NPC1L1 and its correlation with liver X receptor (LXR) expression in peripheral mononuclear (PMN) cells in patients with NAFLD. Methods We evaluated intestinal expression of NPC1L1 in 25 NAFLD patients and 28 healthy controls. We calculated the mRNA expression levels of LXR and farnesoid X receptor (FXR), which are master players of cholesterol metabolism in PMN cells. The protein expression of ABCA1, ABCG5/8, NPC1L1, SREBP, LXR, FXR, and CD36 was measured on tissue samples from the duodenum and ileum. Results The expression of LXR (p = 0.01) and FXR (p = 0.03) in PMN cells was increased in the NAFLD group compared to the control group. Duodenal NPC1L1 decreased in the NAFLD group compared to the healthy controls (3.38 ± 1.4 vs. 2.42 ± 1.2, p = 0.05). NPC1L1 expression in the duodenum was negatively correlated with LXR expression in PMN cells. Expression of LXR and FXR in the ileum was also negatively correlated with the expression of LXR in PMN cells. Conclusions Duodenal NPC1L1 expression was decreased in NAFLD and was negatively correlated with LXR expression in PMN cells.http://www.kjim.org/upload/pdf/kjim-2017-100.pdfliver x receptorsfarnesoid x receptorniemann-pick c1-like 1non-alcoholic fatty liver disease |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sang Bong Ahn Dae Won Jun Kiseok Jang Byung Hoon Lee Kye Jung Shin |
spellingShingle |
Sang Bong Ahn Dae Won Jun Kiseok Jang Byung Hoon Lee Kye Jung Shin Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver disease The Korean Journal of Internal Medicine liver x receptors farnesoid x receptor niemann-pick c1-like 1 non-alcoholic fatty liver disease |
author_facet |
Sang Bong Ahn Dae Won Jun Kiseok Jang Byung Hoon Lee Kye Jung Shin |
author_sort |
Sang Bong Ahn |
title |
Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver disease |
title_short |
Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver disease |
title_full |
Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver disease |
title_fullStr |
Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver disease |
title_full_unstemmed |
Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver disease |
title_sort |
duodenal niemann-pick c1-like 1 expression was negatively correlated with liver x receptor expression in nonalcoholic fatty liver disease |
publisher |
The Korean Association of Internal Medicine |
series |
The Korean Journal of Internal Medicine |
issn |
1226-3303 2005-6648 |
publishDate |
2019-07-01 |
description |
Background/Aims Intestinal cholesterol absorption includes intestinal Niemann-Pick C1-like 1 (NPC1L1) and is an important target pathway in nonalcoholic fatty liver disease (NAFLD). We investigated the expression of NPC1L1 and its correlation with liver X receptor (LXR) expression in peripheral mononuclear (PMN) cells in patients with NAFLD. Methods We evaluated intestinal expression of NPC1L1 in 25 NAFLD patients and 28 healthy controls. We calculated the mRNA expression levels of LXR and farnesoid X receptor (FXR), which are master players of cholesterol metabolism in PMN cells. The protein expression of ABCA1, ABCG5/8, NPC1L1, SREBP, LXR, FXR, and CD36 was measured on tissue samples from the duodenum and ileum. Results The expression of LXR (p = 0.01) and FXR (p = 0.03) in PMN cells was increased in the NAFLD group compared to the control group. Duodenal NPC1L1 decreased in the NAFLD group compared to the healthy controls (3.38 ± 1.4 vs. 2.42 ± 1.2, p = 0.05). NPC1L1 expression in the duodenum was negatively correlated with LXR expression in PMN cells. Expression of LXR and FXR in the ileum was also negatively correlated with the expression of LXR in PMN cells. Conclusions Duodenal NPC1L1 expression was decreased in NAFLD and was negatively correlated with LXR expression in PMN cells. |
topic |
liver x receptors farnesoid x receptor niemann-pick c1-like 1 non-alcoholic fatty liver disease |
url |
http://www.kjim.org/upload/pdf/kjim-2017-100.pdf |
work_keys_str_mv |
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