Enterobacter asburiae Strain L1: Complete Genome and Whole Genome Optical Mapping Analysis of a Quorum Sensing Bacterium

Enterobacter asburiae L1 is a quorum sensing bacterium isolated from lettuce leaves. In this study, for the first time, the complete genome of E. asburiae L1 was sequenced using the single molecule real time sequencer (PacBio RSII) and the whole genome sequence was verified by using optical genome m...

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Main Authors: Yin Yin Lau, Wai-Fong Yin, Kok-Gan Chan
Format: Article
Language:English
Published: MDPI AG 2014-07-01
Series:Sensors
Subjects:
Online Access:http://www.mdpi.com/1424-8220/14/8/13913
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spelling doaj-4820003e8bdb4a87b18bd8991e0655522020-11-24T22:02:30ZengMDPI AGSensors1424-82202014-07-01148139131392410.3390/s140813913s140813913Enterobacter asburiae Strain L1: Complete Genome and Whole Genome Optical Mapping Analysis of a Quorum Sensing BacteriumYin Yin Lau0Wai-Fong Yin1Kok-Gan Chan2Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, MalaysiaDivision of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, MalaysiaDivision of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, MalaysiaEnterobacter asburiae L1 is a quorum sensing bacterium isolated from lettuce leaves. In this study, for the first time, the complete genome of E. asburiae L1 was sequenced using the single molecule real time sequencer (PacBio RSII) and the whole genome sequence was verified by using optical genome mapping (OpGen) technology. In our previous study, E. asburiae L1 has been reported to produce AHLs, suggesting the possibility of virulence factor regulation which is quorum sensing dependent. This evoked our interest to study the genome of this bacterium and here we present the complete genome of E. asburiae L1, which carries the virulence factor gene virK, the N-acyl homoserine lactone-based QS transcriptional regulator gene luxR and the N-acyl homoserine lactone synthase gene which we firstly named easI. The availability of the whole genome sequence of E. asburiae L1 will pave the way for the study of the QS-mediated gene expression in this bacterium. Hence, the importance and functions of these signaling molecules can be further studied in the hope of elucidating the mechanisms of QS-regulation in E. asburiae. To the best of our knowledge, this is the first documentation of both a complete genome sequence and the establishment of the molecular basis of QS properties of E. asburiae.http://www.mdpi.com/1424-8220/14/8/13913Enterobacter asburiaequorum sensingN-acyl homoserine lactone synthasefood spoilagefood safetyvirulence factorsnext-generation sequencing technology
collection DOAJ
language English
format Article
sources DOAJ
author Yin Yin Lau
Wai-Fong Yin
Kok-Gan Chan
spellingShingle Yin Yin Lau
Wai-Fong Yin
Kok-Gan Chan
Enterobacter asburiae Strain L1: Complete Genome and Whole Genome Optical Mapping Analysis of a Quorum Sensing Bacterium
Sensors
Enterobacter asburiae
quorum sensing
N-acyl homoserine lactone synthase
food spoilage
food safety
virulence factors
next-generation sequencing technology
author_facet Yin Yin Lau
Wai-Fong Yin
Kok-Gan Chan
author_sort Yin Yin Lau
title Enterobacter asburiae Strain L1: Complete Genome and Whole Genome Optical Mapping Analysis of a Quorum Sensing Bacterium
title_short Enterobacter asburiae Strain L1: Complete Genome and Whole Genome Optical Mapping Analysis of a Quorum Sensing Bacterium
title_full Enterobacter asburiae Strain L1: Complete Genome and Whole Genome Optical Mapping Analysis of a Quorum Sensing Bacterium
title_fullStr Enterobacter asburiae Strain L1: Complete Genome and Whole Genome Optical Mapping Analysis of a Quorum Sensing Bacterium
title_full_unstemmed Enterobacter asburiae Strain L1: Complete Genome and Whole Genome Optical Mapping Analysis of a Quorum Sensing Bacterium
title_sort enterobacter asburiae strain l1: complete genome and whole genome optical mapping analysis of a quorum sensing bacterium
publisher MDPI AG
series Sensors
issn 1424-8220
publishDate 2014-07-01
description Enterobacter asburiae L1 is a quorum sensing bacterium isolated from lettuce leaves. In this study, for the first time, the complete genome of E. asburiae L1 was sequenced using the single molecule real time sequencer (PacBio RSII) and the whole genome sequence was verified by using optical genome mapping (OpGen) technology. In our previous study, E. asburiae L1 has been reported to produce AHLs, suggesting the possibility of virulence factor regulation which is quorum sensing dependent. This evoked our interest to study the genome of this bacterium and here we present the complete genome of E. asburiae L1, which carries the virulence factor gene virK, the N-acyl homoserine lactone-based QS transcriptional regulator gene luxR and the N-acyl homoserine lactone synthase gene which we firstly named easI. The availability of the whole genome sequence of E. asburiae L1 will pave the way for the study of the QS-mediated gene expression in this bacterium. Hence, the importance and functions of these signaling molecules can be further studied in the hope of elucidating the mechanisms of QS-regulation in E. asburiae. To the best of our knowledge, this is the first documentation of both a complete genome sequence and the establishment of the molecular basis of QS properties of E. asburiae.
topic Enterobacter asburiae
quorum sensing
N-acyl homoserine lactone synthase
food spoilage
food safety
virulence factors
next-generation sequencing technology
url http://www.mdpi.com/1424-8220/14/8/13913
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